Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area
Vibrio cholerae and Vibrio parahaemolyticus are pathogens which cause gastroenteritis and other diseases to human. Wild birds and rodents in our environment play some role in transmitting these pathogenic bacteria to human. Hence, a study was carried out to determine the prevalence of V cholerae and...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2009
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/17710/ http://ir.unimas.my/id/eprint/17710/4/Chong%20Joo%20Hui%20ft.pdf |
| _version_ | 1848838350439448576 |
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| author | Chong, Joo Hui. |
| author_facet | Chong, Joo Hui. |
| author_sort | Chong, Joo Hui. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Vibrio cholerae and Vibrio parahaemolyticus are pathogens which cause gastroenteritis and other diseases to human. Wild birds and rodents in our environment play some role in transmitting these pathogenic bacteria to human. Hence, a study was carried out to determine the prevalence of V cholerae and V parahaemolyticus in wild birds and rodents from disturbed and pristine area. The birds and rodents from disturbed (Ta-ann Naman plantation in Sibu) and pristine habitat (Mt. Mulu) was captured and cloacal swab from each animal were collected. Isolation and identification was done on Thiosulphate Citrate
Bile Salt agar and some biochemical test will be done do determine the bacteria species. Multiplex PCR was carried out to screen for the virulence genes of V cholerae which are tcp, ctx, zot and ace. As for V parahaemolyticus, the virulence gene are tdh and trh. The outcome is that the most of the samples collected from disturbed habitat were found to contain Vibrio spp. as compare to the samples collected from pristine habitat. Besides, not all the isolates of Vibrio cholerae and Vibrio parahaemolyticus contain virulence gene. |
| first_indexed | 2025-11-15T06:54:09Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-17710 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:54:09Z |
| publishDate | 2009 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-177102023-02-08T03:51:52Z http://ir.unimas.my/id/eprint/17710/ Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area Chong, Joo Hui. QR Microbiology QR180 Immunology Vibrio cholerae and Vibrio parahaemolyticus are pathogens which cause gastroenteritis and other diseases to human. Wild birds and rodents in our environment play some role in transmitting these pathogenic bacteria to human. Hence, a study was carried out to determine the prevalence of V cholerae and V parahaemolyticus in wild birds and rodents from disturbed and pristine area. The birds and rodents from disturbed (Ta-ann Naman plantation in Sibu) and pristine habitat (Mt. Mulu) was captured and cloacal swab from each animal were collected. Isolation and identification was done on Thiosulphate Citrate Bile Salt agar and some biochemical test will be done do determine the bacteria species. Multiplex PCR was carried out to screen for the virulence genes of V cholerae which are tcp, ctx, zot and ace. As for V parahaemolyticus, the virulence gene are tdh and trh. The outcome is that the most of the samples collected from disturbed habitat were found to contain Vibrio spp. as compare to the samples collected from pristine habitat. Besides, not all the isolates of Vibrio cholerae and Vibrio parahaemolyticus contain virulence gene. Universiti Malaysia Sarawak, (UNIMAS) 2009 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/17710/4/Chong%20Joo%20Hui%20ft.pdf Chong, Joo Hui. (2009) Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QR Microbiology QR180 Immunology Chong, Joo Hui. Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area |
| title | Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area |
| title_full | Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area |
| title_fullStr | Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area |
| title_full_unstemmed | Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area |
| title_short | Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area |
| title_sort | prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area |
| topic | QR Microbiology QR180 Immunology |
| url | http://ir.unimas.my/id/eprint/17710/ http://ir.unimas.my/id/eprint/17710/4/Chong%20Joo%20Hui%20ft.pdf |