Short homologies efficiently generate detectable homologous recombination events

Short homologies efficiently generate detectable homologous recombination events

When recombineering bacterial artificial chromosomes (BACs), it is common practice to design the ends of the donor molecule with 50 bp of homology specifying its insertion site. We demonstrate that desired recombinants can be produced using intermolecular homologies as short as 15 bp. Although the...

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Bibliographic Details
Main Authors: Osahor, Andrew N., Tan, Chau-Yan, Sim, Edmund U. H., Lee, Choon-Weng, Narayanan, Kulathuramaiyer
Format: Article
Language:English
Published: Academic Press Inc. 2014
Subjects:
QD Chemistry
Online Access:http://ir.unimas.my/id/eprint/17707/
http://ir.unimas.my/id/eprint/17707/1/Short%20homologies%20efficiently%20generate%20detectable%20homologous%20%28abstract%29.pdf

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