Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system

Eight bacterial isolates (B I, B2, B3, B4, B5, B6, B7 and B8) were isolated from the oil and grease trap of a Grey Water Treatment System (ECOSAN System) in Kenanga College. Four of the bacterial isolates were identified based on API 20E system as follows: B2 -Chromobacterium violeceum, B4 -Pseudomo...

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Main Author: Delima, anak Andrew Buntak.
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2008
Subjects:
Online Access:http://ir.unimas.my/id/eprint/17704/
http://ir.unimas.my/id/eprint/17704/1/Delima%20Andrew%20Buntak%20ft.pdf
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author Delima, anak Andrew Buntak.
author_facet Delima, anak Andrew Buntak.
author_sort Delima, anak Andrew Buntak.
building UNIMAS Institutional Repository
collection Online Access
description Eight bacterial isolates (B I, B2, B3, B4, B5, B6, B7 and B8) were isolated from the oil and grease trap of a Grey Water Treatment System (ECOSAN System) in Kenanga College. Four of the bacterial isolates were identified based on API 20E system as follows: B2 -Chromobacterium violeceum, B4 -Pseudomonas aeruginosa, B7 ,-Pseudomonas fluorescent/pulida and B8 -Klebsiella pneumonia pneumonia while three of them were identified based on 16S rRNA sequencing as follows: B3 -Bacillus sp. CNJ782 PL04, B5 -Pseudomonas aeruginosa Strain PDIOO and B6 -Pseudomonas aeruginosa strain RII. However, B 1 was unidentifiable. All the eight isolates were positively screened as oil degrading bacteria by using Rhodamine B agar. B4 can be concluded as having the highest degradation activity when incubated with both 1 % and 2% Tween 20 and Tween 80 compared to the other bacterial isolates.
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format Final Year Project Report / IMRAD
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institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:54:08Z
publishDate 2008
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
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spelling unimas-177042023-07-06T04:28:37Z http://ir.unimas.my/id/eprint/17704/ Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system Delima, anak Andrew Buntak. QR Microbiology Eight bacterial isolates (B I, B2, B3, B4, B5, B6, B7 and B8) were isolated from the oil and grease trap of a Grey Water Treatment System (ECOSAN System) in Kenanga College. Four of the bacterial isolates were identified based on API 20E system as follows: B2 -Chromobacterium violeceum, B4 -Pseudomonas aeruginosa, B7 ,-Pseudomonas fluorescent/pulida and B8 -Klebsiella pneumonia pneumonia while three of them were identified based on 16S rRNA sequencing as follows: B3 -Bacillus sp. CNJ782 PL04, B5 -Pseudomonas aeruginosa Strain PDIOO and B6 -Pseudomonas aeruginosa strain RII. However, B 1 was unidentifiable. All the eight isolates were positively screened as oil degrading bacteria by using Rhodamine B agar. B4 can be concluded as having the highest degradation activity when incubated with both 1 % and 2% Tween 20 and Tween 80 compared to the other bacterial isolates. Universiti Malaysia Sarawak, (UNIMAS) 2008 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/17704/1/Delima%20Andrew%20Buntak%20ft.pdf Delima, anak Andrew Buntak. (2008) Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle QR Microbiology
Delima, anak Andrew Buntak.
Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system
title Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system
title_full Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system
title_fullStr Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system
title_full_unstemmed Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system
title_short Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system
title_sort isolation and characterization of oil degrading bacteria from a local ecological sanitation (ecosan) system
topic QR Microbiology
url http://ir.unimas.my/id/eprint/17704/
http://ir.unimas.my/id/eprint/17704/1/Delima%20Andrew%20Buntak%20ft.pdf