Application of a chromogenic medium and the PCR method for the rapid confirmation and characterization of vibro parahaemolyticus from seafoods
Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar, a chromogenic medium and biochemical test were used to isolate, pre-confirm and identify the presence of 45 Vibrio parahaemolYlicus isolates from 15 samples of 3 different species offish (ikan kembung. ikan bawal and ikan sangeh) in the Kuching-Sam...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2004
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| Online Access: | http://ir.unimas.my/id/eprint/17696/ http://ir.unimas.my/id/eprint/17696/8/Application%20of%20a%20chromogenic%20medium%20and%20the%20PCR%20method%20for%20the%20rapid%20confirmation..%20%28fulltext%29.pdf |
| _version_ | 1848838346579640320 |
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| author | Chong, Wan Chin |
| author_facet | Chong, Wan Chin |
| author_sort | Chong, Wan Chin |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar, a chromogenic medium and biochemical test were used to isolate, pre-confirm and identify the presence of 45 Vibrio parahaemolYlicus isolates from 15 samples of 3 different species offish (ikan kembung. ikan bawal and ikan sangeh) in the Kuching-Samarahan district between March 2003 and July 2003. Among the presumptive isolates, 18 isolates (40%) were randomly chosen to be analyzed using specific PCR amplification targeting the Ihermolabile (II) gene. All 18 isolates generated PCR products of 450 bp in size, thus indicating all presumptive isolates were indeed V. parahaemolYlicus. RAPD-PCR technique was later performed to fingerprint the isolates using primer GEN 15008. Bands ranging from 250 bp to 2500 bp were generated showing polymorphism among the isolates. Data from the PCR reaction were later used to construct a dendogram for c1ustal analysis. Finding shows that the 38 isolates belonged to 2 major clusters containing 7 and 31 isolates, respectively and the Simpson Index of Diversity calculated was 0.909. |
| first_indexed | 2025-11-15T06:54:06Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-17696 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:54:06Z |
| publishDate | 2004 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-176962023-02-16T02:22:59Z http://ir.unimas.my/id/eprint/17696/ Application of a chromogenic medium and the PCR method for the rapid confirmation and characterization of vibro parahaemolyticus from seafoods Chong, Wan Chin QL Zoology SH Aquaculture. Fisheries. Angling Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar, a chromogenic medium and biochemical test were used to isolate, pre-confirm and identify the presence of 45 Vibrio parahaemolYlicus isolates from 15 samples of 3 different species offish (ikan kembung. ikan bawal and ikan sangeh) in the Kuching-Samarahan district between March 2003 and July 2003. Among the presumptive isolates, 18 isolates (40%) were randomly chosen to be analyzed using specific PCR amplification targeting the Ihermolabile (II) gene. All 18 isolates generated PCR products of 450 bp in size, thus indicating all presumptive isolates were indeed V. parahaemolYlicus. RAPD-PCR technique was later performed to fingerprint the isolates using primer GEN 15008. Bands ranging from 250 bp to 2500 bp were generated showing polymorphism among the isolates. Data from the PCR reaction were later used to construct a dendogram for c1ustal analysis. Finding shows that the 38 isolates belonged to 2 major clusters containing 7 and 31 isolates, respectively and the Simpson Index of Diversity calculated was 0.909. Universiti Malaysia Sarawak, (UNIMAS) 2004 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/17696/8/Application%20of%20a%20chromogenic%20medium%20and%20the%20PCR%20method%20for%20the%20rapid%20confirmation..%20%28fulltext%29.pdf Chong, Wan Chin (2004) Application of a chromogenic medium and the PCR method for the rapid confirmation and characterization of vibro parahaemolyticus from seafoods. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QL Zoology SH Aquaculture. Fisheries. Angling Chong, Wan Chin Application of a chromogenic medium and the PCR method for the rapid confirmation and characterization of vibro parahaemolyticus from seafoods |
| title | Application of a chromogenic medium and the PCR method for the rapid confirmation and characterization of vibro parahaemolyticus from seafoods |
| title_full | Application of a chromogenic medium and the PCR method for the rapid confirmation and characterization of vibro parahaemolyticus from seafoods |
| title_fullStr | Application of a chromogenic medium and the PCR method for the rapid confirmation and characterization of vibro parahaemolyticus from seafoods |
| title_full_unstemmed | Application of a chromogenic medium and the PCR method for the rapid confirmation and characterization of vibro parahaemolyticus from seafoods |
| title_short | Application of a chromogenic medium and the PCR method for the rapid confirmation and characterization of vibro parahaemolyticus from seafoods |
| title_sort | application of a chromogenic medium and the pcr method for the rapid confirmation and characterization of vibro parahaemolyticus from seafoods |
| topic | QL Zoology SH Aquaculture. Fisheries. Angling |
| url | http://ir.unimas.my/id/eprint/17696/ http://ir.unimas.my/id/eprint/17696/8/Application%20of%20a%20chromogenic%20medium%20and%20the%20PCR%20method%20for%20the%20rapid%20confirmation..%20%28fulltext%29.pdf |