Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains
Four serotypes of dengue virus (DENV 1–4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100 million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquit...
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| Format: | Article |
| Language: | English |
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BioMed Central
2012
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| Online Access: | http://ir.unimas.my/id/eprint/17689/ http://ir.unimas.my/id/eprint/17689/1/Genetic.pdf |
| _version_ | 1848838344585248768 |
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| author | Rossi, Shannan L. Nasar, F. Cardosa, Mary Jane |
| author_facet | Rossi, Shannan L. Nasar, F. Cardosa, Mary Jane |
| author_sort | Rossi, Shannan L. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Four serotypes of dengue virus (DENV 1–4) currently circulate between humans and domestic/peridomestic
Aedes mosquitoes, resulting in 100 million infections per year. All four serotypes emerged, independently,
from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This
study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from
its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation
of both the 5′- and 3′-untranslated regions but considerable divergence within the open reading
frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human
liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation
within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2,
where human strains produced a larger number of progeny than sylvatic strains in human liver cells but
not in monkey or mosquito cells. |
| first_indexed | 2025-11-15T06:54:04Z |
| format | Article |
| id | unimas-17689 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:54:04Z |
| publishDate | 2012 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-176892022-01-24T06:44:03Z http://ir.unimas.my/id/eprint/17689/ Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains Rossi, Shannan L. Nasar, F. Cardosa, Mary Jane Q Science (General) Four serotypes of dengue virus (DENV 1–4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100 million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5′- and 3′-untranslated regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells. BioMed Central 2012 Article PeerReviewed text en http://ir.unimas.my/id/eprint/17689/1/Genetic.pdf Rossi, Shannan L. and Nasar, F. and Cardosa, Mary Jane (2012) Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains. Virology Journal, 423. pp. 58-67. ISSN 1743-422X http://www.sciencedirect.com/science/article/pii/S0042682211005472 doi:10.1016/j.virol.2011.11.018 |
| spellingShingle | Q Science (General) Rossi, Shannan L. Nasar, F. Cardosa, Mary Jane Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains |
| title | Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains |
| title_full | Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains |
| title_fullStr | Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains |
| title_full_unstemmed | Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains |
| title_short | Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains |
| title_sort | genetic and phenotypic characterization of sylvatic dengue virus type 4 strains |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/17689/ http://ir.unimas.my/id/eprint/17689/ http://ir.unimas.my/id/eprint/17689/ http://ir.unimas.my/id/eprint/17689/1/Genetic.pdf |