Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains
Four serotypes of dengue virus (DENV 1–4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100 million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquit...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BioMed Central
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/17689/ http://ir.unimas.my/id/eprint/17689/1/Genetic.pdf |
| Summary: | Four serotypes of dengue virus (DENV 1–4) currently circulate between humans and domestic/peridomestic
Aedes mosquitoes, resulting in 100 million infections per year. All four serotypes emerged, independently,
from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This
study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from
its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation
of both the 5′- and 3′-untranslated regions but considerable divergence within the open reading
frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human
liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation
within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2,
where human strains produced a larger number of progeny than sylvatic strains in human liver cells but
not in monkey or mosquito cells. |
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