Identification and characterization of pestalotiopsis species using morphological and molecular techniques.

Pestalotiopsis is pathogens which usually cause diseases on stressed plants. It is very hard to define Pestalotiopsis morphologically because Pestalotiopsis is a complex genus and consists of members difficult to classify at the species level. In this work, morphological and molecular characteristic...

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Main Author: Chen, Soon Kiong.
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2007
Subjects:
Online Access:http://ir.unimas.my/id/eprint/17602/
http://ir.unimas.my/id/eprint/17602/4/Chen%20Soon%20Kiong%20ft.pdf
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author Chen, Soon Kiong.
author_facet Chen, Soon Kiong.
author_sort Chen, Soon Kiong.
building UNIMAS Institutional Repository
collection Online Access
description Pestalotiopsis is pathogens which usually cause diseases on stressed plants. It is very hard to define Pestalotiopsis morphologically because Pestalotiopsis is a complex genus and consists of members difficult to classify at the species level. In this work, morphological and molecular characteristics of Pestalotiopsis were studied. The growth of 17 Pestalotiopsis was tested on malt extract agar (MEA). The molecular studies of 13 Pestalotiopsis at species level were assessed by using random amplification of polymorphic DNA (RAPD). There was no significant different on the growth of conidia color and shape. However, the conidia tend to varied in pigmentation of median celts, acervuli production and number of apical appendages ranging from 2 to 4. Phylogenetic analysis proved that Pestalotiopsis can be categorized based on pigmentation of median cells.
first_indexed 2025-11-15T06:53:44Z
format Final Year Project Report / IMRAD
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institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:53:44Z
publishDate 2007
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
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spelling unimas-176022023-02-09T03:53:19Z http://ir.unimas.my/id/eprint/17602/ Identification and characterization of pestalotiopsis species using morphological and molecular techniques. Chen, Soon Kiong. QR Microbiology Pestalotiopsis is pathogens which usually cause diseases on stressed plants. It is very hard to define Pestalotiopsis morphologically because Pestalotiopsis is a complex genus and consists of members difficult to classify at the species level. In this work, morphological and molecular characteristics of Pestalotiopsis were studied. The growth of 17 Pestalotiopsis was tested on malt extract agar (MEA). The molecular studies of 13 Pestalotiopsis at species level were assessed by using random amplification of polymorphic DNA (RAPD). There was no significant different on the growth of conidia color and shape. However, the conidia tend to varied in pigmentation of median celts, acervuli production and number of apical appendages ranging from 2 to 4. Phylogenetic analysis proved that Pestalotiopsis can be categorized based on pigmentation of median cells. Universiti Malaysia Sarawak, (UNIMAS) 2007 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/17602/4/Chen%20Soon%20Kiong%20ft.pdf Chen, Soon Kiong. (2007) Identification and characterization of pestalotiopsis species using morphological and molecular techniques. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle QR Microbiology
Chen, Soon Kiong.
Identification and characterization of pestalotiopsis species using morphological and molecular techniques.
title Identification and characterization of pestalotiopsis species using morphological and molecular techniques.
title_full Identification and characterization of pestalotiopsis species using morphological and molecular techniques.
title_fullStr Identification and characterization of pestalotiopsis species using morphological and molecular techniques.
title_full_unstemmed Identification and characterization of pestalotiopsis species using morphological and molecular techniques.
title_short Identification and characterization of pestalotiopsis species using morphological and molecular techniques.
title_sort identification and characterization of pestalotiopsis species using morphological and molecular techniques.
topic QR Microbiology
url http://ir.unimas.my/id/eprint/17602/
http://ir.unimas.my/id/eprint/17602/4/Chen%20Soon%20Kiong%20ft.pdf