Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters

The genus Pseudo-nitzschia has attracted attention because of production of the toxin, domoic acid (DA), causing Amnesic Shellfish Poisoning (ASP). Pseudo-nitzschia blooms occur frequently in Chinese coastal waters, and DA has been detected in several marine organisms, but so far no Pseudo-nitzsch...

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Main Authors: Yang, Li, Chun, Xiu Huang, Guo, Shuang Xu, Nina, Lundholm, Sing, Tung Teng, Haiyan, Wu, Zhijun, Tan
Format: Article
Language:English
Published: Elsevier Ltd. 2017
Subjects:
Online Access:http://ir.unimas.my/id/eprint/17377/
http://ir.unimas.my/id/eprint/17377/1/Pseudo-nitzschia%20simulans%20sp.%20nov.%20%28Bacillariophyceae%29%20%28abstract%29.pdf
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author Yang, Li
Chun, Xiu Huang
Guo, Shuang Xu
Nina, Lundholm
Sing, Tung Teng
Haiyan, Wu
Zhijun, Tan
author_facet Yang, Li
Chun, Xiu Huang
Guo, Shuang Xu
Nina, Lundholm
Sing, Tung Teng
Haiyan, Wu
Zhijun, Tan
author_sort Yang, Li
building UNIMAS Institutional Repository
collection Online Access
description The genus Pseudo-nitzschia has attracted attention because of production of the toxin, domoic acid (DA), causing Amnesic Shellfish Poisoning (ASP). Pseudo-nitzschia blooms occur frequently in Chinese coastal waters, and DA has been detected in several marine organisms, but so far no Pseudo-nitzschia strains from Chinese waters have been shown to produce DA. In this study, monoclonal Pseudo-nitzschia strains were established from Chinese coastal waters and examined using light microscopy, electron microscopy and molecular markers. Five strains, sharing distinct morphological and molecular features differentiating them from other Pseudo-nitzschia species, represent a new species, Pseudo-nitzschia simulans sp. nov. Morphologically, the taxon belongs to the P. pseudodelicatissima group, cells possessing a central nodule and each stria comprising one row of poroids. The new species is characterized by the poroid structure, which typically comprises two sectors, each sector located near opposite margins of the poroid. The production of DA was examined by liquid chromatography tandem mass spectrometry (LC–MS/MS) analyses of cells in stationary growth phase. Domoic acid was detected in one of the five strains, with concentrations around 1.05–1.54 fg cell�1. This is the first toxigenic diatom species reported from Chinese waters.
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institution Universiti Malaysia Sarawak
institution_category Local University
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publishDate 2017
publisher Elsevier Ltd.
recordtype eprints
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spelling unimas-173772017-10-10T06:29:37Z http://ir.unimas.my/id/eprint/17377/ Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters Yang, Li Chun, Xiu Huang Guo, Shuang Xu Nina, Lundholm Sing, Tung Teng Haiyan, Wu Zhijun, Tan GE Environmental Sciences The genus Pseudo-nitzschia has attracted attention because of production of the toxin, domoic acid (DA), causing Amnesic Shellfish Poisoning (ASP). Pseudo-nitzschia blooms occur frequently in Chinese coastal waters, and DA has been detected in several marine organisms, but so far no Pseudo-nitzschia strains from Chinese waters have been shown to produce DA. In this study, monoclonal Pseudo-nitzschia strains were established from Chinese coastal waters and examined using light microscopy, electron microscopy and molecular markers. Five strains, sharing distinct morphological and molecular features differentiating them from other Pseudo-nitzschia species, represent a new species, Pseudo-nitzschia simulans sp. nov. Morphologically, the taxon belongs to the P. pseudodelicatissima group, cells possessing a central nodule and each stria comprising one row of poroids. The new species is characterized by the poroid structure, which typically comprises two sectors, each sector located near opposite margins of the poroid. The production of DA was examined by liquid chromatography tandem mass spectrometry (LC–MS/MS) analyses of cells in stationary growth phase. Domoic acid was detected in one of the five strains, with concentrations around 1.05–1.54 fg cell�1. This is the first toxigenic diatom species reported from Chinese waters. Elsevier Ltd. 2017 Article PeerReviewed text en http://ir.unimas.my/id/eprint/17377/1/Pseudo-nitzschia%20simulans%20sp.%20nov.%20%28Bacillariophyceae%29%20%28abstract%29.pdf Yang, Li and Chun, Xiu Huang and Guo, Shuang Xu and Nina, Lundholm and Sing, Tung Teng and Haiyan, Wu and Zhijun, Tan (2017) Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters. Harmful Algae, 67. pp. 119-130. ISSN 1568-9883 http://dx.doi.org/10.1016/j.hal.2017.06.008 http://dx.doi.org/10.1016/j.hal.2017.06.008
spellingShingle GE Environmental Sciences
Yang, Li
Chun, Xiu Huang
Guo, Shuang Xu
Nina, Lundholm
Sing, Tung Teng
Haiyan, Wu
Zhijun, Tan
Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters
title Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters
title_full Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters
title_fullStr Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters
title_full_unstemmed Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters
title_short Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters
title_sort pseudo-nitzschia simulans sp. nov. (bacillariophyceae), the first domoic acid producer from chinese waters
topic GE Environmental Sciences
url http://ir.unimas.my/id/eprint/17377/
http://ir.unimas.my/id/eprint/17377/
http://ir.unimas.my/id/eprint/17377/
http://ir.unimas.my/id/eprint/17377/1/Pseudo-nitzschia%20simulans%20sp.%20nov.%20%28Bacillariophyceae%29%20%28abstract%29.pdf