Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis
Sample preparation for scanning electron microscope analysis involves reagents and equipment that are expensive and often hazardous. Here we demonstrate a circumvention of Osmium tetroxide and critical point drying, greatly reducing the duration, complexity and cost of the process. We captured early...
| Main Authors: | , , , , , |
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| Format: | Article |
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Academic Press Inc.
2017
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| Online Access: | http://ir.unimas.my/id/eprint/17178/ |
| _version_ | 1848838229911928832 |
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| author | Andrew, Osahor Karthik, Deekonda Choon, Weng Lee Ui, Edmund Hang Sim Aurelian, Radu Kumaran, Narayanan |
| author_facet | Andrew, Osahor Karthik, Deekonda Choon, Weng Lee Ui, Edmund Hang Sim Aurelian, Radu Kumaran, Narayanan |
| author_sort | Andrew, Osahor |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Sample preparation for scanning electron microscope analysis involves reagents and equipment that are expensive and often hazardous. Here we demonstrate a circumvention of Osmium tetroxide and critical point drying, greatly reducing the duration, complexity and cost of the process. We captured early stage interactions of invasive-bacteria and HeLa cells during the process of bacteria-mediated gene delivery and illustrate sufficient clarity can be obtained using this procedure to preserve and clearly visualize relevant cellular structures. This protocol is significantly cheaper and easier to adapt compared to conventional methods, and will allow routine preparation/viewing of eukaryotic or bacterial samples for basic morphological studies. |
| first_indexed | 2025-11-15T06:52:15Z |
| format | Article |
| id | unimas-17178 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| last_indexed | 2025-11-15T06:52:15Z |
| publishDate | 2017 |
| publisher | Academic Press Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-171782017-10-13T00:33:34Z http://ir.unimas.my/id/eprint/17178/ Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis Andrew, Osahor Karthik, Deekonda Choon, Weng Lee Ui, Edmund Hang Sim Aurelian, Radu Kumaran, Narayanan QR Microbiology Sample preparation for scanning electron microscope analysis involves reagents and equipment that are expensive and often hazardous. Here we demonstrate a circumvention of Osmium tetroxide and critical point drying, greatly reducing the duration, complexity and cost of the process. We captured early stage interactions of invasive-bacteria and HeLa cells during the process of bacteria-mediated gene delivery and illustrate sufficient clarity can be obtained using this procedure to preserve and clearly visualize relevant cellular structures. This protocol is significantly cheaper and easier to adapt compared to conventional methods, and will allow routine preparation/viewing of eukaryotic or bacterial samples for basic morphological studies. Academic Press Inc. 2017 Article PeerReviewed Andrew, Osahor and Karthik, Deekonda and Choon, Weng Lee and Ui, Edmund Hang Sim and Aurelian, Radu and Kumaran, Narayanan (2017) Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis. Analytical Biochemistry, 534. pp. 46-48. ISSN 0003-2697 http://www.sciencedirect.com/science/article/pii/S0003269717302920 https://doi.org/10.1016/j.ab.2017.07.008 |
| spellingShingle | QR Microbiology Andrew, Osahor Karthik, Deekonda Choon, Weng Lee Ui, Edmund Hang Sim Aurelian, Radu Kumaran, Narayanan Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis |
| title | Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis |
| title_full | Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis |
| title_fullStr | Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis |
| title_full_unstemmed | Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis |
| title_short | Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis |
| title_sort | rapid preparation of adherent mammalian cells for basic scanning electron microscopy (sem) analysis |
| topic | QR Microbiology |
| url | http://ir.unimas.my/id/eprint/17178/ http://ir.unimas.my/id/eprint/17178/ http://ir.unimas.my/id/eprint/17178/ |