Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae
Magnaporthe oryzae is a fungal pathogen contributing to rice blast diseases globally via their Avr (avirulence) gene. Although the occurrence of M. oryzae has been reported in Sarawak since several decades ago, however, none has focused specifically on Avr genes, which confer resistance against pa...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2017
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/17160/ http://ir.unimas.my/id/eprint/17160/7/Isolation.pdf |
| _version_ | 1848838224809558016 |
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| author | Mui, Sie Jee Whye, Leonard Kit Lim Martina Azelin, anak Dirum Sara, Ilia Che, Hashim Muhammad Shafiq, Masri Hui, Ying Tan Lee, San Lai Kuok, Freddy San Yeo Hung, Hui Chung |
| author_facet | Mui, Sie Jee Whye, Leonard Kit Lim Martina Azelin, anak Dirum Sara, Ilia Che, Hashim Muhammad Shafiq, Masri Hui, Ying Tan Lee, San Lai Kuok, Freddy San Yeo Hung, Hui Chung |
| author_sort | Mui, Sie Jee |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Magnaporthe oryzae is a fungal pathogen contributing to rice blast diseases globally via their Avr (avirulence)
gene. Although the occurrence of M. oryzae has been reported in Sarawak since several decades ago, however,
none has focused specifically on Avr genes, which confer resistance against pathogen-associated molecular
pattern-triggered immunity (PTI) in host. The objective of this study is to isolate Avr genes from M. oryzae 7’ (a
Sarawak isolate) that may contribute to susceptibility of rice towards diseases. In this study, AvrPiz-t, AVR-Pik,
Avr-Pi54, and AVR-Pita1 genes were isolated via PCR and cloning approaches. The genes were then compared
with set of similar genes from related isolates derived from NCBI. Results revealed that all eight Avr genes
(including four other global isolates) shared similar N-myristoylation site and a novel motif. 3D modeling
revealed similar β-sandwich structure in AvrPiz-t and AVR-Pik despite sequence dissimilarities. In conclusion, it
is confirmed of the presence of these genes in the Sarawak (M. oryzae) isolate. This study implies that Sarawak
isolate may confer similar avirulence properties as their counterparts worldwide. Further R/Avr gene-for-gene
relationship studies may aid in strategic control of rice blast diseases in future. |
| first_indexed | 2025-11-15T06:52:10Z |
| format | Article |
| id | unimas-17160 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:52:10Z |
| publishDate | 2017 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-171602022-06-21T07:59:17Z http://ir.unimas.my/id/eprint/17160/ Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae Mui, Sie Jee Whye, Leonard Kit Lim Martina Azelin, anak Dirum Sara, Ilia Che, Hashim Muhammad Shafiq, Masri Hui, Ying Tan Lee, San Lai Kuok, Freddy San Yeo Hung, Hui Chung S Agriculture (General) Magnaporthe oryzae is a fungal pathogen contributing to rice blast diseases globally via their Avr (avirulence) gene. Although the occurrence of M. oryzae has been reported in Sarawak since several decades ago, however, none has focused specifically on Avr genes, which confer resistance against pathogen-associated molecular pattern-triggered immunity (PTI) in host. The objective of this study is to isolate Avr genes from M. oryzae 7’ (a Sarawak isolate) that may contribute to susceptibility of rice towards diseases. In this study, AvrPiz-t, AVR-Pik, Avr-Pi54, and AVR-Pita1 genes were isolated via PCR and cloning approaches. The genes were then compared with set of similar genes from related isolates derived from NCBI. Results revealed that all eight Avr genes (including four other global isolates) shared similar N-myristoylation site and a novel motif. 3D modeling revealed similar β-sandwich structure in AvrPiz-t and AVR-Pik despite sequence dissimilarities. In conclusion, it is confirmed of the presence of these genes in the Sarawak (M. oryzae) isolate. This study implies that Sarawak isolate may confer similar avirulence properties as their counterparts worldwide. Further R/Avr gene-for-gene relationship studies may aid in strategic control of rice blast diseases in future. Universiti Malaysia Sarawak, (UNIMAS) 2017 Article PeerReviewed text en http://ir.unimas.my/id/eprint/17160/7/Isolation.pdf Mui, Sie Jee and Whye, Leonard Kit Lim and Martina Azelin, anak Dirum and Sara, Ilia and Che, Hashim and Muhammad Shafiq, Masri and Hui, Ying Tan and Lee, San Lai and Kuok, Freddy San Yeo and Hung, Hui Chung (2017) Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae. Borneo Journal of Resource Science and Technology, 7 (1). pp. 31-42. ISSN 2229-9769 |
| spellingShingle | S Agriculture (General) Mui, Sie Jee Whye, Leonard Kit Lim Martina Azelin, anak Dirum Sara, Ilia Che, Hashim Muhammad Shafiq, Masri Hui, Ying Tan Lee, San Lai Kuok, Freddy San Yeo Hung, Hui Chung Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae |
| title | Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae |
| title_full | Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae |
| title_fullStr | Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae |
| title_full_unstemmed | Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae |
| title_short | Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae |
| title_sort | isolation and characterization of avirulence genes in magnaporthe oryzae |
| topic | S Agriculture (General) |
| url | http://ir.unimas.my/id/eprint/17160/ http://ir.unimas.my/id/eprint/17160/7/Isolation.pdf |