Molecular And 3D-Structural Characterization Of Fructose-1,6-Bisphosphate Aldolase Derived From Metroxylon Sagu
Fructose-1,6-bisphosphate aldolase (FBAld) is an enzyme that catalyzes the cleavage of D-fructose-1,6-phosphate (FBP) to D-glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP), and plays vital role in glycolysis and gluconeogenesis. However, molecular characterization and functiona...
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| Format: | Article |
| Language: | English |
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SCIELO
2017
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| Online Access: | http://ir.unimas.my/id/eprint/17018/ http://ir.unimas.my/id/eprint/17018/1/Molecular_And_3D-Structural_Characterization_Of_Fr%28Abstract%29.pdf |
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| author | Hairul Azman, Roslan Md. Anowar, Hossain Jerry, anak Gerunsin |
| author_facet | Hairul Azman, Roslan Md. Anowar, Hossain Jerry, anak Gerunsin |
| author_sort | Hairul Azman, Roslan |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Fructose-1,6-bisphosphate aldolase (FBAld) is an enzyme that catalyzes the cleavage of D-fructose-1,6-phosphate (FBP) to D-glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP), and plays vital role in glycolysis and gluconeogenesis. However, molecular characterization and functional roles of FBAld remain unknown in sago palm. Here we report a modified CTAB-RNA extraction method was developed for the isolation of good quality RNA (RIN>8) from sago leaves and the isolation of FBAld cDNA from sago palm. The isolated sago FBAld (msFBAld) cDNA has total length of 1288 bp with an open reading frame of 1020 bp and a predicted to encode for a protein of 340 amino acid resides. The predicted protein shared a high degree of homology with Class-I FBAld from other plants. Meanwhile, the msFBAld gene spanned 2322 bp and consisted of five exons. Conserved domain search identified fifteen catalytically important amino acids at the active site and phylogenetic tree revealed localization of msFBAld in the chloroplast. A molecular 3D-structure of msFBAld was generated by homology modeling and a Ramachandran plot with 86.7% of the residues in the core region, 13.4% in the allowed region with no residues in the disallowed region. The modeled structure is a homotetramer containing an /-TIM-barrel at the center. Superimposition of the model with Class-I aldolases identified a catalytic dyad, Lys209-Glu167, which could be involved in the Schiff's base formation and aldol condensation. Apart from that, overproduction of the recombinant msFBAld in Escherichia coli resulted in increased tolerance towards salinity. |
| first_indexed | 2025-11-15T06:51:33Z |
| format | Article |
| id | unimas-17018 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:51:33Z |
| publishDate | 2017 |
| publisher | SCIELO |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-170182017-08-09T06:47:14Z http://ir.unimas.my/id/eprint/17018/ Molecular And 3D-Structural Characterization Of Fructose-1,6-Bisphosphate Aldolase Derived From Metroxylon Sagu Hairul Azman, Roslan Md. Anowar, Hossain Jerry, anak Gerunsin Q Science (General) Fructose-1,6-bisphosphate aldolase (FBAld) is an enzyme that catalyzes the cleavage of D-fructose-1,6-phosphate (FBP) to D-glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP), and plays vital role in glycolysis and gluconeogenesis. However, molecular characterization and functional roles of FBAld remain unknown in sago palm. Here we report a modified CTAB-RNA extraction method was developed for the isolation of good quality RNA (RIN>8) from sago leaves and the isolation of FBAld cDNA from sago palm. The isolated sago FBAld (msFBAld) cDNA has total length of 1288 bp with an open reading frame of 1020 bp and a predicted to encode for a protein of 340 amino acid resides. The predicted protein shared a high degree of homology with Class-I FBAld from other plants. Meanwhile, the msFBAld gene spanned 2322 bp and consisted of five exons. Conserved domain search identified fifteen catalytically important amino acids at the active site and phylogenetic tree revealed localization of msFBAld in the chloroplast. A molecular 3D-structure of msFBAld was generated by homology modeling and a Ramachandran plot with 86.7% of the residues in the core region, 13.4% in the allowed region with no residues in the disallowed region. The modeled structure is a homotetramer containing an /-TIM-barrel at the center. Superimposition of the model with Class-I aldolases identified a catalytic dyad, Lys209-Glu167, which could be involved in the Schiff's base formation and aldol condensation. Apart from that, overproduction of the recombinant msFBAld in Escherichia coli resulted in increased tolerance towards salinity. SCIELO 2017 Article PeerReviewed text en http://ir.unimas.my/id/eprint/17018/1/Molecular_And_3D-Structural_Characterization_Of_Fr%28Abstract%29.pdf Hairul Azman, Roslan and Md. Anowar, Hossain and Jerry, anak Gerunsin (2017) Molecular And 3D-Structural Characterization Of Fructose-1,6-Bisphosphate Aldolase Derived From Metroxylon Sagu. Brazilian Archives of Biology and Technology, 60. ISSN 1516-8913 http://dx.doi.org/10.1590/1678-4324-2017160108 10.1590/1678-4324-2017160108 |
| spellingShingle | Q Science (General) Hairul Azman, Roslan Md. Anowar, Hossain Jerry, anak Gerunsin Molecular And 3D-Structural Characterization Of Fructose-1,6-Bisphosphate Aldolase Derived From Metroxylon Sagu |
| title | Molecular And 3D-Structural Characterization Of Fructose-1,6-Bisphosphate Aldolase Derived From Metroxylon Sagu |
| title_full | Molecular And 3D-Structural Characterization Of Fructose-1,6-Bisphosphate Aldolase Derived From Metroxylon Sagu |
| title_fullStr | Molecular And 3D-Structural Characterization Of Fructose-1,6-Bisphosphate Aldolase Derived From Metroxylon Sagu |
| title_full_unstemmed | Molecular And 3D-Structural Characterization Of Fructose-1,6-Bisphosphate Aldolase Derived From Metroxylon Sagu |
| title_short | Molecular And 3D-Structural Characterization Of Fructose-1,6-Bisphosphate Aldolase Derived From Metroxylon Sagu |
| title_sort | molecular and 3d-structural characterization of fructose-1,6-bisphosphate aldolase derived from metroxylon sagu |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/17018/ http://ir.unimas.my/id/eprint/17018/ http://ir.unimas.my/id/eprint/17018/ http://ir.unimas.my/id/eprint/17018/1/Molecular_And_3D-Structural_Characterization_Of_Fr%28Abstract%29.pdf |