Overexpression of Wildtype Periostin and Transforming Growth Factor Beta I Genes in Colorectal Carcinoma: A Preliminary Study
The majority of deaths from colorectal carcinoma (CRC) occur due to metastasis during the late stage of tumourigenesis. Recently, periostin, i.e. a gene encoding a protein which is initially found in osteoblasts, has been reported to be associated with the late-stage tumourigenesis in colon and a...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia
2009
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| Online Access: | http://ir.unimas.my/id/eprint/16915/ http://ir.unimas.my/id/eprint/16915/1/Overexpression.pdf |
| _version_ | 1848838168145559552 |
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| author | Chia, Sze Wooi Sim, Edmund U. H. |
| author_facet | Chia, Sze Wooi Sim, Edmund U. H. |
| author_sort | Chia, Sze Wooi |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The majority of deaths from colorectal carcinoma (CRC) occur due to metastasis during the late stage of
tumourigenesis. Recently, periostin, i.e. a gene encoding a protein which is initially found in osteoblasts, has
been reported to be associated with the late-stage tumourigenesis in colon and a variety of human cancers. The
researchers investigated the expression of periostin mRNA in normal and tumour biopsy specimens using the
RT-PCR analysis to elucidate the role of periostin in human colorectal carcinoma. The results showed that there
was a significantly (P<0.05) higher expression of the periostin mRNA in the biopsy specimens obtained from
the tumour tissues, as compared to the normal tissues. Nevertheless, sequence analysis revealed no mutation
in the full length of the periostin gene. As the over-expression of periostin in human colorectal carcinoma did
not appear to be due to the mutation in the periostin gene, the involvement of other collaborative factors was
therefore deduced. Consistent with this finding, the researchers focussed on studying the transforming growth
factor (TGF) b1 which has been reported to be associated with the increasing in the expression of periostin.
The analysis (RT-PCR) in this study revealed that TGF- b1 gene was also highly expressed in tumour biopsy
specimens (P<0.05). This gene mutation is also absent. These data validated that both periostin and TGF- b1
work together to control colorectal organogenesis. |
| first_indexed | 2025-11-15T06:51:16Z |
| format | Article |
| id | unimas-16915 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:51:16Z |
| publishDate | 2009 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-169152022-01-20T01:34:16Z http://ir.unimas.my/id/eprint/16915/ Overexpression of Wildtype Periostin and Transforming Growth Factor Beta I Genes in Colorectal Carcinoma: A Preliminary Study Chia, Sze Wooi Sim, Edmund U. H. Q Science (General) R Medicine (General) The majority of deaths from colorectal carcinoma (CRC) occur due to metastasis during the late stage of tumourigenesis. Recently, periostin, i.e. a gene encoding a protein which is initially found in osteoblasts, has been reported to be associated with the late-stage tumourigenesis in colon and a variety of human cancers. The researchers investigated the expression of periostin mRNA in normal and tumour biopsy specimens using the RT-PCR analysis to elucidate the role of periostin in human colorectal carcinoma. The results showed that there was a significantly (P<0.05) higher expression of the periostin mRNA in the biopsy specimens obtained from the tumour tissues, as compared to the normal tissues. Nevertheless, sequence analysis revealed no mutation in the full length of the periostin gene. As the over-expression of periostin in human colorectal carcinoma did not appear to be due to the mutation in the periostin gene, the involvement of other collaborative factors was therefore deduced. Consistent with this finding, the researchers focussed on studying the transforming growth factor (TGF) b1 which has been reported to be associated with the increasing in the expression of periostin. The analysis (RT-PCR) in this study revealed that TGF- b1 gene was also highly expressed in tumour biopsy specimens (P<0.05). This gene mutation is also absent. These data validated that both periostin and TGF- b1 work together to control colorectal organogenesis. Universiti Putra Malaysia 2009 Article PeerReviewed text en http://ir.unimas.my/id/eprint/16915/1/Overexpression.pdf Chia, Sze Wooi and Sim, Edmund U. H. (2009) Overexpression of Wildtype Periostin and Transforming Growth Factor Beta I Genes in Colorectal Carcinoma: A Preliminary Study. Pertanika Journal of Tropical Agricultural Science, 32 (2). pp. 153-159. ISSN 1511-3701 https://www.researchgate.net/publication/287885442 |
| spellingShingle | Q Science (General) R Medicine (General) Chia, Sze Wooi Sim, Edmund U. H. Overexpression of Wildtype Periostin and Transforming Growth Factor Beta I Genes in Colorectal Carcinoma: A Preliminary Study |
| title | Overexpression of Wildtype Periostin and Transforming Growth Factor Beta I Genes in Colorectal Carcinoma: A Preliminary Study |
| title_full | Overexpression of Wildtype Periostin and Transforming Growth Factor Beta I Genes in Colorectal Carcinoma: A Preliminary Study |
| title_fullStr | Overexpression of Wildtype Periostin and Transforming Growth Factor Beta I Genes in Colorectal Carcinoma: A Preliminary Study |
| title_full_unstemmed | Overexpression of Wildtype Periostin and Transforming Growth Factor Beta I Genes in Colorectal Carcinoma: A Preliminary Study |
| title_short | Overexpression of Wildtype Periostin and Transforming Growth Factor Beta I Genes in Colorectal Carcinoma: A Preliminary Study |
| title_sort | overexpression of wildtype periostin and transforming growth factor beta i genes in colorectal carcinoma: a preliminary study |
| topic | Q Science (General) R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/16915/ http://ir.unimas.my/id/eprint/16915/ http://ir.unimas.my/id/eprint/16915/1/Overexpression.pdf |