Efficient and precise engineering of a 200 kb b-globin
human/bacterial artificial chromosome in E. coli DH10B
using an inducible homologous recombination system

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Efficient and precise engineering of a 200 kb b-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system

Gene therapy studies require techniques that allow alter- adapted an inducible homologous recombination system ation of human genomic DNA sequences. Bacterial arti- for use in E. coli DH10B cells, the host strain for BACs ficial chromosome cloning systems (BACs/PACs) bridge and PACs. Using this sy...

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Bibliographic Details
Main Authors:	Narayanan, Kulathuramaiyer, Williamson, R., Zhang, Y., Stewart, AF, Ioannou, PA
Format:	Article
Language:	English
Published:	Stockton Press 1999
Subjects:	Q Science (General)
Online Access:	http://ir.unimas.my/id/eprint/16746/ http://ir.unimas.my/id/eprint/16746/1/Efficient.pdf

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