Viability of shorea resinosa foxw excisedembryos following dehydration technique

This study was carried out to assess the viability of cryopreserved excised-embryos of Shorea resinosa Foxw that treated using dehydration technique. Embryos sources were of high quality, where initial moisture content and germination of were 64.9 % and 73 % respectively, and Viability of the excise...

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Bibliographic Details
Main Author: Asrol Hadri, Bin Radzuan.
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2006
Subjects:
Online Access:http://ir.unimas.my/id/eprint/16689/
http://ir.unimas.my/id/eprint/16689/8/Viability%20of%20shorea%20resinosa%20foxw%20excised-embryos%20following%20dehydration%20technique%20%28fulltext%29.pdf
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Summary:This study was carried out to assess the viability of cryopreserved excised-embryos of Shorea resinosa Foxw that treated using dehydration technique. Embryos sources were of high quality, where initial moisture content and germination of were 64.9 % and 73 % respectively, and Viability of the excisedembryos was 93 % when assessed using 0.5 % of tetrazolium for 90 minutes. Results showed that using MS medium with several modifications of composition especially in composition of Macronutriet composition which gave 73% ofgermination. The best treatment was obtained from dehydration of excised embr}Qs with 0.4 M concentration of sucrose for 90 minutes which gave viability of 82 % and exposing excised-embryOs with silica gel by 3 hours which gave 70 %. This result was useful for expand the studies in storage an preserve the embryos in liquid nitrogen