An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf>
Non toxicity and high isoelectric point of zinc oxide nanorods (ZnO NRs) are the promising materials widely used in biomolecule detection and biomedicine application. An electrochemical DNA biosensor was successfully fabricated by depositing (3-Aminopropyl)triethoxysilane and gold nanoparticles (GNP...
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| Format: | Article |
| Language: | English |
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Springer Verlag
2017
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| Online Access: | http://ir.unimas.my/id/eprint/16688/ http://ir.unimas.my/id/eprint/16688/1/An%20electrochemical%20DNA%20biosensor%20based%20gold%E2%80%91thiolate%20%28abstract%29.pdf |
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| author | Foo, K.L. Muhammad Fayyaz, Kashif Tan, S.J. Hashim, U. |
| author_facet | Foo, K.L. Muhammad Fayyaz, Kashif Tan, S.J. Hashim, U. |
| author_sort | Foo, K.L. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Non toxicity and high isoelectric point of zinc oxide nanorods (ZnO NRs) are the promising materials widely used in biomolecule detection and biomedicine application. An electrochemical DNA biosensor was successfully fabricated by depositing (3-Aminopropyl)triethoxysilane and gold nanoparticles (GNPs) on the hydrothermal growth ZnO NRs. The modified surface with GNPs (35 ± 5 nm) was characterized by field emission scanning electron microscopy with energy dispersive X-ray and X-ray diffraction. The fabricated electrode was used for the label-free detection of DNA. Thiolated (–SH) single stranded DNA was immobilized onto the GNPs surface to form a gold-thiolate bond. Hybridization with complementary target DNA was performed with the presence of ruthenium complex. Immobilization and hybridization detection were performed using µAUTOLAB with cyclic voltammetric software. The measured sensitivity of the fabricated electrode for hybridization is up to 1.6 times larger than immobilization. © 2016, Springer-Verlag Berlin Heidelberg. |
| first_indexed | 2025-11-15T06:50:25Z |
| format | Article |
| id | unimas-16688 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:50:25Z |
| publishDate | 2017 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-166882017-06-20T00:40:42Z http://ir.unimas.my/id/eprint/16688/ An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf> Foo, K.L. Muhammad Fayyaz, Kashif Tan, S.J. Hashim, U. TK Electrical engineering. Electronics Nuclear engineering Non toxicity and high isoelectric point of zinc oxide nanorods (ZnO NRs) are the promising materials widely used in biomolecule detection and biomedicine application. An electrochemical DNA biosensor was successfully fabricated by depositing (3-Aminopropyl)triethoxysilane and gold nanoparticles (GNPs) on the hydrothermal growth ZnO NRs. The modified surface with GNPs (35 ± 5 nm) was characterized by field emission scanning electron microscopy with energy dispersive X-ray and X-ray diffraction. The fabricated electrode was used for the label-free detection of DNA. Thiolated (–SH) single stranded DNA was immobilized onto the GNPs surface to form a gold-thiolate bond. Hybridization with complementary target DNA was performed with the presence of ruthenium complex. Immobilization and hybridization detection were performed using µAUTOLAB with cyclic voltammetric software. The measured sensitivity of the fabricated electrode for hybridization is up to 1.6 times larger than immobilization. © 2016, Springer-Verlag Berlin Heidelberg. Springer Verlag 2017-05-01 Article PeerReviewed text en http://ir.unimas.my/id/eprint/16688/1/An%20electrochemical%20DNA%20biosensor%20based%20gold%E2%80%91thiolate%20%28abstract%29.pdf Foo, K.L. and Muhammad Fayyaz, Kashif and Tan, S.J. and Hashim, U. (2017) An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf>. Microsystem Technologies, 23 (5). pp. 1237-1245. ISSN 09467076 https://www.springerprofessional.de/en/an-electrochemical-dna-biosensor-based-gold-thiolate-conjugation/7499824 DOI: 10.1007/s00542-016-2874-7 |
| spellingShingle | TK Electrical engineering. Electronics Nuclear engineering Foo, K.L. Muhammad Fayyaz, Kashif Tan, S.J. Hashim, U. An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf> |
| title | An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf> |
| title_full | An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf> |
| title_fullStr | An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf> |
| title_full_unstemmed | An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf> |
| title_short | An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)<inf>2</inf>(qtpy)]Cl<inf>2</inf> |
| title_sort | electrochemical dna biosensor based gold-thiolate conjugation utilizing ruthenium complex [ru(dppz)<inf>2</inf>(qtpy)]cl<inf>2</inf> |
| topic | TK Electrical engineering. Electronics Nuclear engineering |
| url | http://ir.unimas.my/id/eprint/16688/ http://ir.unimas.my/id/eprint/16688/ http://ir.unimas.my/id/eprint/16688/ http://ir.unimas.my/id/eprint/16688/1/An%20electrochemical%20DNA%20biosensor%20based%20gold%E2%80%91thiolate%20%28abstract%29.pdf |