The hexane fraction of Ardisia crispa Thunb. A. DC. roots inhibits inflammation-induced angiogenesis
Background: Ardisia crispa (Myrsinaceae) is used in traditional Malay medicine to treat various ailments associated with inflammation, including rheumatism. The plant's hexane fraction was previously shown to inhibit several diseases associated with inflammation. As there is a strong correlatio...
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| Format: | Article |
| Language: | English |
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BioMed Central
2013
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| Online Access: | http://ir.unimas.my/id/eprint/16425/ http://ir.unimas.my/id/eprint/16425/1/The%20hexane%20fraction%20of%20Ardisia%20crispa%20Thunb.%20A.%20DC.%20roots%20inhibits%20inflammation-induced%20angiogenesis%20%28abstrak%29.pdf |
| _version_ | 1848838064493821952 |
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| author | Dayang Erna Zulaikha, Awang Hamsin Roslida, Abdul Hamid Latifah, Saiful Yazan Che Norma, Mat Taib Yeong, Looi Ting |
| author_facet | Dayang Erna Zulaikha, Awang Hamsin Roslida, Abdul Hamid Latifah, Saiful Yazan Che Norma, Mat Taib Yeong, Looi Ting |
| author_sort | Dayang Erna Zulaikha, Awang Hamsin |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Background: Ardisia crispa (Myrsinaceae) is used in traditional Malay medicine to treat various ailments associated with inflammation, including rheumatism. The plant's hexane fraction was previously shown to inhibit several diseases associated with inflammation. As there is a strong correlation between inflammation and angiogenesis, we conducted the present study to investigate the anti-angiogenic effects of the plant's roots in animal models of inflammation-induced angiogenesis.Methods: We first performed phytochemical screening and high-performance liquid chromatography (HPLC) fingerprinting of the hexane fraction of Ardisia crispa roots ethanolic extract (ACRH) and its quinone-rich fraction (QRF). The anti-inflammatory properties of ACRH and QRF were tested using the Miles vascular permeability assay and the murine air pouch granuloma model following oral administration at various doses.Results: Preliminary phytochemical screening of ACRH revealed the presence of flavonoids, triterpenes, and tannins. The QRF was separated from ACRH (38.38% w/w) by column chromatography, and was isolated to yield a benzoquinonoid compound. The ACRH and QRF were quantified by HPLC. The LD50 value of ACRH was 617.02 mg/kg. In the Miles vascular permeability assay, the lowest dose of ACRH (10 mg/kg) and all doses of QRF significantly reduced vascular endothelial growth factor (VEGF)-induced hyperpermeability, when compared with the vehicle control. In the murine air pouch granuloma model, ACRH and QRF both displayed significant and dose-dependent anti-inflammatory effects, without granuloma weight. ACRH and QRF significantly reduced the vascular index, but not granuloma tissue weight.Conclusions: In conclusion, both ACRH and QRF showed potential anti-inflammatory properties in a model of inflammation-induced angiogenesis model, demonstrating their potential anti-angiogenic properties |
| first_indexed | 2025-11-15T06:49:37Z |
| format | Article |
| id | unimas-16425 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:49:37Z |
| publishDate | 2013 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-164252017-05-24T07:29:45Z http://ir.unimas.my/id/eprint/16425/ The hexane fraction of Ardisia crispa Thunb. A. DC. roots inhibits inflammation-induced angiogenesis Dayang Erna Zulaikha, Awang Hamsin Roslida, Abdul Hamid Latifah, Saiful Yazan Che Norma, Mat Taib Yeong, Looi Ting RS Pharmacy and materia medica Background: Ardisia crispa (Myrsinaceae) is used in traditional Malay medicine to treat various ailments associated with inflammation, including rheumatism. The plant's hexane fraction was previously shown to inhibit several diseases associated with inflammation. As there is a strong correlation between inflammation and angiogenesis, we conducted the present study to investigate the anti-angiogenic effects of the plant's roots in animal models of inflammation-induced angiogenesis.Methods: We first performed phytochemical screening and high-performance liquid chromatography (HPLC) fingerprinting of the hexane fraction of Ardisia crispa roots ethanolic extract (ACRH) and its quinone-rich fraction (QRF). The anti-inflammatory properties of ACRH and QRF were tested using the Miles vascular permeability assay and the murine air pouch granuloma model following oral administration at various doses.Results: Preliminary phytochemical screening of ACRH revealed the presence of flavonoids, triterpenes, and tannins. The QRF was separated from ACRH (38.38% w/w) by column chromatography, and was isolated to yield a benzoquinonoid compound. The ACRH and QRF were quantified by HPLC. The LD50 value of ACRH was 617.02 mg/kg. In the Miles vascular permeability assay, the lowest dose of ACRH (10 mg/kg) and all doses of QRF significantly reduced vascular endothelial growth factor (VEGF)-induced hyperpermeability, when compared with the vehicle control. In the murine air pouch granuloma model, ACRH and QRF both displayed significant and dose-dependent anti-inflammatory effects, without granuloma weight. ACRH and QRF significantly reduced the vascular index, but not granuloma tissue weight.Conclusions: In conclusion, both ACRH and QRF showed potential anti-inflammatory properties in a model of inflammation-induced angiogenesis model, demonstrating their potential anti-angiogenic properties BioMed Central 2013-01 Article PeerReviewed text en http://ir.unimas.my/id/eprint/16425/1/The%20hexane%20fraction%20of%20Ardisia%20crispa%20Thunb.%20A.%20DC.%20roots%20inhibits%20inflammation-induced%20angiogenesis%20%28abstrak%29.pdf Dayang Erna Zulaikha, Awang Hamsin and Roslida, Abdul Hamid and Latifah, Saiful Yazan and Che Norma, Mat Taib and Yeong, Looi Ting (2013) The hexane fraction of Ardisia crispa Thunb. A. DC. roots inhibits inflammation-induced angiogenesis. BMC Complementary and Alternative Medicine, 13 (8). p. 5. ISSN 1472-6882 https://bmccomplementalternmed.biomedcentral.com/ DOI: 10.1186/1472-6882-13-5 |
| spellingShingle | RS Pharmacy and materia medica Dayang Erna Zulaikha, Awang Hamsin Roslida, Abdul Hamid Latifah, Saiful Yazan Che Norma, Mat Taib Yeong, Looi Ting The hexane fraction of Ardisia crispa Thunb. A. DC. roots inhibits inflammation-induced angiogenesis |
| title | The hexane fraction of Ardisia crispa Thunb. A. DC. roots inhibits inflammation-induced angiogenesis |
| title_full | The hexane fraction of Ardisia crispa Thunb. A. DC. roots inhibits inflammation-induced angiogenesis |
| title_fullStr | The hexane fraction of Ardisia crispa Thunb. A. DC. roots inhibits inflammation-induced angiogenesis |
| title_full_unstemmed | The hexane fraction of Ardisia crispa Thunb. A. DC. roots inhibits inflammation-induced angiogenesis |
| title_short | The hexane fraction of Ardisia crispa Thunb. A. DC. roots inhibits inflammation-induced angiogenesis |
| title_sort | hexane fraction of ardisia crispa thunb. a. dc. roots inhibits inflammation-induced angiogenesis |
| topic | RS Pharmacy and materia medica |
| url | http://ir.unimas.my/id/eprint/16425/ http://ir.unimas.my/id/eprint/16425/ http://ir.unimas.my/id/eprint/16425/ http://ir.unimas.my/id/eprint/16425/1/The%20hexane%20fraction%20of%20Ardisia%20crispa%20Thunb.%20A.%20DC.%20roots%20inhibits%20inflammation-induced%20angiogenesis%20%28abstrak%29.pdf |