Site Directed Mutagenesis of StIsa2 Gene and
Expression of the Recombinant Enzyme in E. coli

QR Code

Site Directed Mutagenesis of StIsa2 Gene and Expression of the Recombinant Enzyme in E. coli

Isoamylase is a type of starch debranching enzyme (DBE) that debranches glucan polymers producing linear maltooligosaccharides during starch biosynthesis in plants. Three isoamylase isoforms exist, and each plays a distinct and important, but yet unclear role in starch biosynthesis. However, isoa...

Full description

Bibliographic Details
Main Authors:	Fatt, Nikson Ming Chong, Mohd. Hasnain, Hussain
Format:	Proceeding
Language:	English
Published:	2013
Subjects:	Q Science (General)
Online Access:	http://ir.unimas.my/id/eprint/16325/ http://ir.unimas.my/id/eprint/16325/1/Nikson%20Chong.pdf

Similar Items

Implications of stisa2 catalytic residue restoration through site directed mutagenesis [Bölgeye yönlendirilmiş mutajenez ile stisa2 katalitik amino asit restorasyonunun olasi sonuçlari]
by: Hasnain, Hussain, et al.
Published: (2017)

Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis
by: Hasnain, Hussain, et al.
Published: (2016)

Site directed mutagenesis to improve E. Coli phytase activity for animal feed
by: Samsudin, Nurhusna, et al.
Published: (2012)

Production of recombinant p53 protein by PCR-based site directed mutagenesis of plasmid DNA
by: Bakhtiar, M. Taher, et al.
Published: (2010)

Site-directed mutagenesis of superfolder green fluorescent protein
by: Lee, Sock Im
Published: (2015)

Heterologous Expression Of Xylanase Gene From Klebsiella Pneumoniae In E. Coli Bl21 (De3) For Potential Use In Green Technology
by: Mohd Hasnain, Hussain, et al.
Published: (2013)

Site-directed mutagenesis on plasmid using polymerase chain reaction
by: Ahmad, Abdul Aziz, et al.
Published: (2018)

Site-directed mutagenesis of helical lid of J15 lipase
by: Ho, Chern Min
Published: (2015)

Recombinant Enzyme: Cloning and Expression
by: Amid, Azura, et al.
Published: (2015)

Bioprocessing of recombinant E.Coli producing Glucuronidase Enzymes
by: Mel, Maizirwan
Published: (2012)

Site directed mutagenesis to improve phyfauia1 phytase activity for animal feed
by: Mohd. Salleh, Hamzah, et al.
Published: (2011)

Site-directed mutagenesis of newcastle disease VIRUS V protein in virus pathogenicity
by: Tham, May Ling
Published: (2021)

Site-directed mutagenesis of n-terminal Alpha Helix of J15 lipase
by: Lee, Mei Ying
Published: (2015)

Site-directed mutagenesis of newcastle disease VIRUS V protein in virus pathogenicity
by: Tham, May Ling
Published: (2021)

Site-directed mutagenesis of the C-terminal of the Newcastle disease virus V protein
by: Tham, May Ling, et al.
Published: (2022)

Bioprocessing of recombinant E.Coli producing β- glucuronidase enzyme
Published: (2011)

Site directed mutagenesis of miRNA binding site on the 3′-UTR of choline kinase alpha gene
by: Bnisallama, Faisal Fayez Saffah
Published: (2022)

Xylanase gene from indigenous Bacillus sp.
by: Hasnain, Hussain, et al.
Published: (2010)

Site-directed mutagenesis to determine the role of surface exposed lysine on the stability of staphylococcal lipase
by: Ahmad, Nurul Nadirah
Published: (2020)

Crossflow microfiltration of recombinant Escherichia coli producing β-glucuronidase enzyme
by: Mel, Maizirwan, et al.
Published: (2011)

Expression and the antigenicity of recombinant coat proteins of tungro viruses expressed in Escherichia coli
by: Yee, Siewfung, et al.
Published: (2017)

Computational site-directed mutagenesis studies of the role of the hydrophobic triad on substrate binding in cholesterol oxidase
by: Harb, L., et al.
Published: (2017)

Identification of important residues for catalysis and substrate specificity in human choline kinase by site directed mutagenesis
by: Wong Mun, Teng
Published: (2008)

Characterisation of Klebsiella pneumoniae Xylanase and Increment of Its Activity in Heterologous Expression System
by: Mohd Hasnain, Hussain, et al.
Published: (2016)

Site-directed mutagenesis for the production of mutant TP53 gene and analysis of its tumor suppressor activity
by: Bakhtiar, M. Taher, et al.
Published: (2012)

Optimization of signal peptide via site-directed mutagenesis for enhanced secretion of heterologous proteins in lactococcus lactis
by: Alias, Nur Aqlili Riana, et al.
Published: (2022)

Recombinant expression of functional mouse AhR LBD in E. coli
by: Elhawari, Wail
Published: (2012)

Cloning and purification of recombinant proteins of Mycoplasma hyopneumoniae expressed in Escherichia coli
by: Simionatto, S., et al.
Published: (2010)

Isolation, Cloning and Expression of Recombinant Human Renin in Escherichia Coli System
by: Ng, Chyan Leong
Published: (2002)

Cloning and Expression of Recombinant Human Epidermal Growth Factor In Escherichia Coli
by: Abdull Razis, Ahmad Faizal
Published: (2007)

Improvement of recombinant E.coli fermentation producing β-glucuronidase enzyme by Taguchi's design
by: Mel, Maizirwan, et al.
Published: (2011)

The kLa evaluation of recombinant Escherichia coli fermentation producing β-glucuronidase enzyme
by: Mel, Maizirwan, et al.
Published: (2011)

Development of CRISPR-Cas9-mediated site-directed mutagenesis of ECE1 gene (candidalysin) in Candida albicans
by: Engku Nasrullah Satiman, Engku Anis Fariha, et al.
Published: (2020)

Molecular cloning, modeling and site-directed mutagenesis of type III polyketide synthase from Sargassum binderi (Phaeophyta)
by: Baharum, Hariyanti, et al.
Published: (2011)

Site directed mutagenesis on highly conserved amino acid residues of choline kinase gene from neisseria meningitidis
by: Xiang, Cheong Jing
Published: (2022)

The evaluation of kLa values for recombinant Escherichia coli fermentation producing β-Glucuronidase enzyme
by: Mel, Maizirwan, et al.
Published: (2010)

Development of new media formulation for the improvement of recombinant protein expression in E.Coli
by: Mohd. Salleh, Hamzah, et al.
Published: (2012)

Optimization of inoculum size & agitation rate on expression of recombinant chitinase in Escherichia coli
by: Nor Fadzillah Hanis, Abu Sulaiman
Published: (2009)

Periplasmic protein changes in response to over-expression of recombinant protein in escherichia coli
by: Heidary, Somayyeh
Published: (2013)

Recombinant-enzyme fermentation
by: Amid, Azura, et al.
Published: (2015)