Escherichia coli bactofection using Lipofectamine
Successful gene delivery into mammalian cells using bactofection requires entry of the bacterial vector via cell surface integrin receptors followed by release of plasmid DNA into the cellular environment. We show, for the first time, that addition of the DNA transfection reagent Lipofectamine impro...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Academic Press Inc.
2013
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| Online Access: | http://ir.unimas.my/id/eprint/16310/ http://ir.unimas.my/id/eprint/16310/1/Escherichia%20coli%20bactofection%20using%20Lipofectamine%20%28abstrak%29.pdf |
| _version_ | 1848838034197315584 |
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| author | Kumaran, Narayanan Choon, Weng Lee Aurelian, Radu Edmund, Ui Hang Sim |
| author_facet | Kumaran, Narayanan Choon, Weng Lee Aurelian, Radu Edmund, Ui Hang Sim |
| author_sort | Kumaran, Narayanan |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Successful gene delivery into mammalian cells using bactofection requires entry of the bacterial vector via cell surface integrin receptors followed by release of plasmid DNA into the cellular environment. We show, for the first time, that addition of the DNA transfection reagent Lipofectamine improves entry of invasive Escherichia coli into HeLa cells and enhances up to 2.8-fold green fluorescent protein (GFP) expression from a reporter plasmid. The addition of Lipofectamine may be applicable to other bacterial vectors to increase their DNA delivery efficiency into mammalian cells. |
| first_indexed | 2025-11-15T06:49:08Z |
| format | Article |
| id | unimas-16310 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:49:08Z |
| publishDate | 2013 |
| publisher | Academic Press Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-163102017-05-18T01:44:07Z http://ir.unimas.my/id/eprint/16310/ Escherichia coli bactofection using Lipofectamine Kumaran, Narayanan Choon, Weng Lee Aurelian, Radu Edmund, Ui Hang Sim QR Microbiology Successful gene delivery into mammalian cells using bactofection requires entry of the bacterial vector via cell surface integrin receptors followed by release of plasmid DNA into the cellular environment. We show, for the first time, that addition of the DNA transfection reagent Lipofectamine improves entry of invasive Escherichia coli into HeLa cells and enhances up to 2.8-fold green fluorescent protein (GFP) expression from a reporter plasmid. The addition of Lipofectamine may be applicable to other bacterial vectors to increase their DNA delivery efficiency into mammalian cells. Academic Press Inc. 2013 Article PeerReviewed text en http://ir.unimas.my/id/eprint/16310/1/Escherichia%20coli%20bactofection%20using%20Lipofectamine%20%28abstrak%29.pdf Kumaran, Narayanan and Choon, Weng Lee and Aurelian, Radu and Edmund, Ui Hang Sim (2013) Escherichia coli bactofection using Lipofectamine. Analytical Biochemistry, 439 (2). pp. 142-144. ISSN 0003-2697 http://www.sciencedirect.com/science/journal/00032697 DOI: 10.1016/j.ab.2013.04.010 |
| spellingShingle | QR Microbiology Kumaran, Narayanan Choon, Weng Lee Aurelian, Radu Edmund, Ui Hang Sim Escherichia coli bactofection using Lipofectamine |
| title | Escherichia coli bactofection using Lipofectamine |
| title_full | Escherichia coli bactofection using Lipofectamine |
| title_fullStr | Escherichia coli bactofection using Lipofectamine |
| title_full_unstemmed | Escherichia coli bactofection using Lipofectamine |
| title_short | Escherichia coli bactofection using Lipofectamine |
| title_sort | escherichia coli bactofection using lipofectamine |
| topic | QR Microbiology |
| url | http://ir.unimas.my/id/eprint/16310/ http://ir.unimas.my/id/eprint/16310/ http://ir.unimas.my/id/eprint/16310/ http://ir.unimas.my/id/eprint/16310/1/Escherichia%20coli%20bactofection%20using%20Lipofectamine%20%28abstrak%29.pdf |