Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch
A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. Thi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer Berlin Heidelberg
2009
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| Online Access: | http://ir.unimas.my/id/eprint/16285/ http://ir.unimas.my/id/eprint/16285/1/Production%20of%20nisin%20Z%20using%20Lactococcus%20lactis%20%28abstract%29.pdf |
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| author | Octavio, Carvajal-Zarrabal Cirilo, Nolasco-Hipolito Kopli, Bujang Ayaaki, Ishizaki |
| author_facet | Octavio, Carvajal-Zarrabal Cirilo, Nolasco-Hipolito Kopli, Bujang Ayaaki, Ishizaki |
| author_sort | Octavio, Carvajal-Zarrabal |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A membrane bioreactor for production of nisin
Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon
source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volumetric productivity of nisin Z, as 50,000 IU l-1 h-1 using a cell concentration of 15 g l-1, 30 � C, pH 5.5 and a dilution rate of 1.24 h-1. Adding 10 g l-1 YE and 2 g l-1 polypeptone, other inducers were unnecessary to maintain production of nisin. The operating conditions of the reactor removed nisin and lactate, thus minimizing their effects which allowed the maintenance of cells in continuous exponential growth phase mode with high metabolic activity. |
| first_indexed | 2025-11-15T06:49:01Z |
| format | Article |
| id | unimas-16285 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:49:01Z |
| publishDate | 2009 |
| publisher | Springer Berlin Heidelberg |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-162852017-05-17T07:26:53Z http://ir.unimas.my/id/eprint/16285/ Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch Octavio, Carvajal-Zarrabal Cirilo, Nolasco-Hipolito Kopli, Bujang Ayaaki, Ishizaki QR Microbiology S Agriculture (General) A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volumetric productivity of nisin Z, as 50,000 IU l-1 h-1 using a cell concentration of 15 g l-1, 30 � C, pH 5.5 and a dilution rate of 1.24 h-1. Adding 10 g l-1 YE and 2 g l-1 polypeptone, other inducers were unnecessary to maintain production of nisin. The operating conditions of the reactor removed nisin and lactate, thus minimizing their effects which allowed the maintenance of cells in continuous exponential growth phase mode with high metabolic activity. Springer Berlin Heidelberg 2009 Article PeerReviewed text en http://ir.unimas.my/id/eprint/16285/1/Production%20of%20nisin%20Z%20using%20Lactococcus%20lactis%20%28abstract%29.pdf Octavio, Carvajal-Zarrabal and Cirilo, Nolasco-Hipolito and Kopli, Bujang and Ayaaki, Ishizaki (2009) Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch. Journal of Industrial Microbiology & Biotechnology, 36 (3). pp. 409-415. ISSN 1476-5535 (online) https://link.springer.com/article/10.1007%2Fs10295-008-0511-x DOI: 10.1007/s10295-008-0511-x |
| spellingShingle | QR Microbiology S Agriculture (General) Octavio, Carvajal-Zarrabal Cirilo, Nolasco-Hipolito Kopli, Bujang Ayaaki, Ishizaki Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch |
| title | Production of nisin Z using Lactococcus lactis IO-1
from hydrolyzed sago starch |
| title_full | Production of nisin Z using Lactococcus lactis IO-1
from hydrolyzed sago starch |
| title_fullStr | Production of nisin Z using Lactococcus lactis IO-1
from hydrolyzed sago starch |
| title_full_unstemmed | Production of nisin Z using Lactococcus lactis IO-1
from hydrolyzed sago starch |
| title_short | Production of nisin Z using Lactococcus lactis IO-1
from hydrolyzed sago starch |
| title_sort | production of nisin z using lactococcus lactis io-1
from hydrolyzed sago starch |
| topic | QR Microbiology S Agriculture (General) |
| url | http://ir.unimas.my/id/eprint/16285/ http://ir.unimas.my/id/eprint/16285/ http://ir.unimas.my/id/eprint/16285/ http://ir.unimas.my/id/eprint/16285/1/Production%20of%20nisin%20Z%20using%20Lactococcus%20lactis%20%28abstract%29.pdf |