Evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of Pichia stipitis and its respiratory deficient
The use of lignocellulosic residues for ethanol production is limited by toxic compounds in fermenting yeasts present in diluted acid hydrolysates like acetic acid and 2-furaldehyde. The respiratory deficient phenotype gives the cell the ability to resist several toxic compounds. So the aim of this...
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| Format: | Article |
| Language: | English English |
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Springer-Verlag Berlin Heidelberg
2014
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| Online Access: | http://ir.unimas.my/id/eprint/16281/ http://ir.unimas.my/id/eprint/16281/1/Evaluation%20of%20the%20tolerance%20of%20acetic%20acid%20and%202-furaldehyde%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/16281/7/Azham.pdf |
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| author | B., Ortiz-Muñiz J., Rasgado-Mellado J., Solis-Pacheco Cirilo Nolasco, Nolasco-Hipólito J. M., Domínguez-González |
| author_facet | B., Ortiz-Muñiz J., Rasgado-Mellado J., Solis-Pacheco Cirilo Nolasco, Nolasco-Hipólito J. M., Domínguez-González |
| author_sort | B., Ortiz-Muñiz |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The use of lignocellulosic residues for ethanol production is limited by toxic compounds in fermenting yeasts present in diluted acid hydrolysates like acetic acid and 2-furaldehyde. The respiratory deficient phenotype gives the cell the ability to resist several toxic compounds. So the aim of this work was to evaluate the tolerance to toxic compounds present in lignocellulosic hydrolysates like acetic acid and 2-furaldehyde in Pichia stipitis and its respiratory deficient strains. The respiratory deficient phenotype was induced by exposure to chemical agents such as acriflavine, acrylamide and rhodamine; 23 strains were obtained. The selection criterion was based on increasing specific ethanol yield (g ethanol g−1 biomass) with acetic acid and furaldehyde tolerance. The screening showed that P. stipitis NRRL Y-7124 ACL 2-1RD (lacking cytochrome c), obtained using acrylamide, presented the highest specific ethanol production rate (1.82 g g−1 h−1). Meanwhile, the ACF8-3RD strain showed the highest acetic acid tolerance (7.80 g L−1) and the RHO2-3RD strain was able to tolerate up to 1.5 g L−1 2-furaldehyde with a growth and ethanol production inhibition of 23 and 22 %, respectively. The use of respiratory deficient yeast phenotype is a strategy for ethanol production improvement in a medium with toxic compounds such as hydrolysed sugarcane bagasse amongst others. |
| first_indexed | 2025-11-15T06:49:01Z |
| format | Article |
| id | unimas-16281 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:49:01Z |
| publishDate | 2014 |
| publisher | Springer-Verlag Berlin Heidelberg |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-162812022-01-25T08:39:38Z http://ir.unimas.my/id/eprint/16281/ Evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of Pichia stipitis and its respiratory deficient B., Ortiz-Muñiz J., Rasgado-Mellado J., Solis-Pacheco Cirilo Nolasco, Nolasco-Hipólito J. M., Domínguez-González QD Chemistry The use of lignocellulosic residues for ethanol production is limited by toxic compounds in fermenting yeasts present in diluted acid hydrolysates like acetic acid and 2-furaldehyde. The respiratory deficient phenotype gives the cell the ability to resist several toxic compounds. So the aim of this work was to evaluate the tolerance to toxic compounds present in lignocellulosic hydrolysates like acetic acid and 2-furaldehyde in Pichia stipitis and its respiratory deficient strains. The respiratory deficient phenotype was induced by exposure to chemical agents such as acriflavine, acrylamide and rhodamine; 23 strains were obtained. The selection criterion was based on increasing specific ethanol yield (g ethanol g−1 biomass) with acetic acid and furaldehyde tolerance. The screening showed that P. stipitis NRRL Y-7124 ACL 2-1RD (lacking cytochrome c), obtained using acrylamide, presented the highest specific ethanol production rate (1.82 g g−1 h−1). Meanwhile, the ACF8-3RD strain showed the highest acetic acid tolerance (7.80 g L−1) and the RHO2-3RD strain was able to tolerate up to 1.5 g L−1 2-furaldehyde with a growth and ethanol production inhibition of 23 and 22 %, respectively. The use of respiratory deficient yeast phenotype is a strategy for ethanol production improvement in a medium with toxic compounds such as hydrolysed sugarcane bagasse amongst others. Springer-Verlag Berlin Heidelberg 2014 Article PeerReviewed text en http://ir.unimas.my/id/eprint/16281/1/Evaluation%20of%20the%20tolerance%20of%20acetic%20acid%20and%202-furaldehyde%20%28abstract%29.pdf text en http://ir.unimas.my/id/eprint/16281/7/Azham.pdf B., Ortiz-Muñiz and J., Rasgado-Mellado and J., Solis-Pacheco and Cirilo Nolasco, Nolasco-Hipólito and J. M., Domínguez-González (2014) Evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of Pichia stipitis and its respiratory deficient. Bioprocess and Biosystems Engineering, 37 (10). pp. 2061-2066. ISSN 1615-7605 (Online) https://link.springer.com/article/10.1007/s00449-014-1183-8 DOI: 10.1007/s00449-014-1183-8 |
| spellingShingle | QD Chemistry B., Ortiz-Muñiz J., Rasgado-Mellado J., Solis-Pacheco Cirilo Nolasco, Nolasco-Hipólito J. M., Domínguez-González Evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of Pichia stipitis and its respiratory deficient |
| title | Evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of Pichia stipitis and its respiratory deficient |
| title_full | Evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of Pichia stipitis and its respiratory deficient |
| title_fullStr | Evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of Pichia stipitis and its respiratory deficient |
| title_full_unstemmed | Evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of Pichia stipitis and its respiratory deficient |
| title_short | Evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of Pichia stipitis and its respiratory deficient |
| title_sort | evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of pichia stipitis and its respiratory deficient |
| topic | QD Chemistry |
| url | http://ir.unimas.my/id/eprint/16281/ http://ir.unimas.my/id/eprint/16281/ http://ir.unimas.my/id/eprint/16281/ http://ir.unimas.my/id/eprint/16281/1/Evaluation%20of%20the%20tolerance%20of%20acetic%20acid%20and%202-furaldehyde%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/16281/7/Azham.pdf |