Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia
Background As Indonesia works towards the goal of malaria elimination, information is lacking on malaria epidemiology from some western provinces. As a basis for studies of antimalarial efficacy, we set out to survey parasite carriage in three communities in North Sumatera Province. Methods A...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2017
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| Online Access: | http://ir.unimas.my/id/eprint/16221/ http://ir.unimas.my/id/eprint/16221/1/Contribution%20of%20Plasmodium%20knowlesi%20to%20multi-species%20%28abstract%29.pdf |
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| author | Inke, ND Lubis Hendri, Wijaya Munar, Lubis Chairuddin P, Lubis Paul Cliff, Simon Divis Khalid, B Beshir Sutherland, Colin J |
| author_facet | Inke, ND Lubis Hendri, Wijaya Munar, Lubis Chairuddin P, Lubis Paul Cliff, Simon Divis Khalid, B Beshir Sutherland, Colin J |
| author_sort | Inke, ND Lubis |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Background
As Indonesia works towards the goal of malaria elimination, information is lacking on malaria
epidemiology from some western provinces. As a basis for studies of antimalarial efficacy, we set out
to survey parasite carriage in three communities in North Sumatera Province.
Methods
A combination of active and passive detection of infection was carried out among communities in
Batubara, Langkat and South Nias regencies. Finger-prick blood samples from consenting individuals
of all ages provided blood films for microscopic examination and blood spots on filter paper.
Plasmodium species were identified by nested PCR of rRNA genes, and a novel assay which amplifies
a conserved sequence specific for the sicavar gene family of P. knowlesi.
Results
614 of 3,731 participants (16.5%) were positive for malaria parasites by microscopy. PCR detected
parasite DNA in samples from 1,169 individuals (31.3%). In total, 377 participants (11.8%) harboured
P. knowlesi. Also present were P. vivax (14.3%), P. falciparum (10.5%) and P. malariae (3.4%).
Conclusions
Amplification of sicavar is a specific and sensitive test for the presence of P. knowlesi DNA in
humans. Subpatent and asymptomatic multi-species parasitaemia is relatively common in North
Sumatera, and so PCR-based surveillance is required to support control and elimination activities. |
| first_indexed | 2025-11-15T06:48:46Z |
| format | Article |
| id | unimas-16221 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:48:46Z |
| publishDate | 2017 |
| publisher | Oxford University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-162212017-05-25T00:41:39Z http://ir.unimas.my/id/eprint/16221/ Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia Inke, ND Lubis Hendri, Wijaya Munar, Lubis Chairuddin P, Lubis Paul Cliff, Simon Divis Khalid, B Beshir Sutherland, Colin J R Medicine (General) Background As Indonesia works towards the goal of malaria elimination, information is lacking on malaria epidemiology from some western provinces. As a basis for studies of antimalarial efficacy, we set out to survey parasite carriage in three communities in North Sumatera Province. Methods A combination of active and passive detection of infection was carried out among communities in Batubara, Langkat and South Nias regencies. Finger-prick blood samples from consenting individuals of all ages provided blood films for microscopic examination and blood spots on filter paper. Plasmodium species were identified by nested PCR of rRNA genes, and a novel assay which amplifies a conserved sequence specific for the sicavar gene family of P. knowlesi. Results 614 of 3,731 participants (16.5%) were positive for malaria parasites by microscopy. PCR detected parasite DNA in samples from 1,169 individuals (31.3%). In total, 377 participants (11.8%) harboured P. knowlesi. Also present were P. vivax (14.3%), P. falciparum (10.5%) and P. malariae (3.4%). Conclusions Amplification of sicavar is a specific and sensitive test for the presence of P. knowlesi DNA in humans. Subpatent and asymptomatic multi-species parasitaemia is relatively common in North Sumatera, and so PCR-based surveillance is required to support control and elimination activities. Oxford University Press 2017 Article PeerReviewed text en http://ir.unimas.my/id/eprint/16221/1/Contribution%20of%20Plasmodium%20knowlesi%20to%20multi-species%20%28abstract%29.pdf Inke, ND Lubis and Hendri, Wijaya and Munar, Lubis and Chairuddin P, Lubis and Paul Cliff, Simon Divis and Khalid, B Beshir and Sutherland, Colin J (2017) Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia. The Journal of Infectious Diseases. ISSN 1537-6613 https://www.researchgate.net/publication/313777791 DOI: 10.1093/infdis/jix091 |
| spellingShingle | R Medicine (General) Inke, ND Lubis Hendri, Wijaya Munar, Lubis Chairuddin P, Lubis Paul Cliff, Simon Divis Khalid, B Beshir Sutherland, Colin J Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia |
| title | Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia |
| title_full | Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia |
| title_fullStr | Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia |
| title_full_unstemmed | Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia |
| title_short | Contribution of Plasmodium knowlesi to multi-species human malaria infections in North Sumatera, Indonesia |
| title_sort | contribution of plasmodium knowlesi to multi-species human malaria infections in north sumatera, indonesia |
| topic | R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/16221/ http://ir.unimas.my/id/eprint/16221/ http://ir.unimas.my/id/eprint/16221/ http://ir.unimas.my/id/eprint/16221/1/Contribution%20of%20Plasmodium%20knowlesi%20to%20multi-species%20%28abstract%29.pdf |