High-resolution mapping of genes involved in plant stage-specific partial resistance of barley to leaf rust
Partial resistance quantitative trait loci (QTLs) Rphq11 and rphq16 against Puccinia hordei isolate 1.2.1 were previously mapped in seedlings of the mapping populations Steptoe/Morex and Oregon Wolfe Barleys, respectively. In this study, QTL mapping was performed at adult plant stage for the two map...
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| Format: | Article |
| Language: | English |
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Springer Netherlands
2017
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| Online Access: | http://ir.unimas.my/id/eprint/16111/ http://ir.unimas.my/id/eprint/16111/7/High-resolution%20mapping%20of%20genes%20involved%20in%20plant%20%28abstract%29.pdf |
| _version_ | 1848837989125324800 |
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| author | Yeo, F. K S Bouchon, R. Kuijken, René C P Loriaux, A. Boyd, C. Niks, Rients E. Marcel, Thierry C. |
| author_facet | Yeo, F. K S Bouchon, R. Kuijken, René C P Loriaux, A. Boyd, C. Niks, Rients E. Marcel, Thierry C. |
| author_sort | Yeo, F. K S |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Partial resistance quantitative trait loci (QTLs) Rphq11 and rphq16 against Puccinia hordei isolate 1.2.1 were previously mapped in seedlings of the mapping populations Steptoe/Morex and Oregon Wolfe Barleys, respectively. In this study, QTL mapping was performed at adult plant stage for the two mapping populations challenged with the same rust isolate. The results suggest that Rphq11 and rphq16 are effective only at seedling stage, and not at adult plant stage. The cloning of several genes responsible for partial resistance of barley to P. hordei will allow elucidation of the molecular basis of this type of plant defence. A map-based cloning approach requires to fine-map the QTL in a narrow genetic window. In this study, Rphq11 and rphq16 were fine-mapped using an approach aiming at speeding up the development of plant material and simplifying its evaluation. The plant materials for fine-mapping were identified from early plant materials developed to produce QTL-NILs. The material was first selected to carry the targeted QTL in heterozygous condition and susceptibility alleles at other resistance QTLs in homozygous condition. This strategy took four to five generations to obtain fixed QTL recombinants (i.e., homozygous resistant at the Rphq11 or rphq16 QTL alleles, homozygous susceptible at the non-targeted QTL alleles). In less than 2 years, Rphq11 was fine-mapped into a 0.2-cM genetic interval and a 1.4-cM genetic interval for rphq16. The strongest candidate gene for Rphq11 is a phospholipid hydroperoxide glutathione peroxidase. Thus far, no candidate gene was identified for rphq16. |
| first_indexed | 2025-11-15T06:48:25Z |
| format | Article |
| id | unimas-16111 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:48:25Z |
| publishDate | 2017 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-161112017-05-04T08:05:20Z http://ir.unimas.my/id/eprint/16111/ High-resolution mapping of genes involved in plant stage-specific partial resistance of barley to leaf rust Yeo, F. K S Bouchon, R. Kuijken, René C P Loriaux, A. Boyd, C. Niks, Rients E. Marcel, Thierry C. S Agriculture (General) SB Plant culture Partial resistance quantitative trait loci (QTLs) Rphq11 and rphq16 against Puccinia hordei isolate 1.2.1 were previously mapped in seedlings of the mapping populations Steptoe/Morex and Oregon Wolfe Barleys, respectively. In this study, QTL mapping was performed at adult plant stage for the two mapping populations challenged with the same rust isolate. The results suggest that Rphq11 and rphq16 are effective only at seedling stage, and not at adult plant stage. The cloning of several genes responsible for partial resistance of barley to P. hordei will allow elucidation of the molecular basis of this type of plant defence. A map-based cloning approach requires to fine-map the QTL in a narrow genetic window. In this study, Rphq11 and rphq16 were fine-mapped using an approach aiming at speeding up the development of plant material and simplifying its evaluation. The plant materials for fine-mapping were identified from early plant materials developed to produce QTL-NILs. The material was first selected to carry the targeted QTL in heterozygous condition and susceptibility alleles at other resistance QTLs in homozygous condition. This strategy took four to five generations to obtain fixed QTL recombinants (i.e., homozygous resistant at the Rphq11 or rphq16 QTL alleles, homozygous susceptible at the non-targeted QTL alleles). In less than 2 years, Rphq11 was fine-mapped into a 0.2-cM genetic interval and a 1.4-cM genetic interval for rphq16. The strongest candidate gene for Rphq11 is a phospholipid hydroperoxide glutathione peroxidase. Thus far, no candidate gene was identified for rphq16. Springer Netherlands 2017-04-01 Article PeerReviewed text en http://ir.unimas.my/id/eprint/16111/7/High-resolution%20mapping%20of%20genes%20involved%20in%20plant%20%28abstract%29.pdf Yeo, F. K S and Bouchon, R. and Kuijken, René C P and Loriaux, A. and Boyd, C. and Niks, Rients E. and Marcel, Thierry C. (2017) High-resolution mapping of genes involved in plant stage-specific partial resistance of barley to leaf rust. Molecular Breeding, 37 (4). ISSN 1380-3743 https://link.springer.com/article/10.1007%2Fs11032-017-0624-x DOI: 10.1007/s11032-017-0624-x |
| spellingShingle | S Agriculture (General) SB Plant culture Yeo, F. K S Bouchon, R. Kuijken, René C P Loriaux, A. Boyd, C. Niks, Rients E. Marcel, Thierry C. High-resolution mapping of genes involved in plant stage-specific partial resistance of barley to leaf rust |
| title | High-resolution mapping of genes involved in plant stage-specific partial resistance of barley to leaf rust |
| title_full | High-resolution mapping of genes involved in plant stage-specific partial resistance of barley to leaf rust |
| title_fullStr | High-resolution mapping of genes involved in plant stage-specific partial resistance of barley to leaf rust |
| title_full_unstemmed | High-resolution mapping of genes involved in plant stage-specific partial resistance of barley to leaf rust |
| title_short | High-resolution mapping of genes involved in plant stage-specific partial resistance of barley to leaf rust |
| title_sort | high-resolution mapping of genes involved in plant stage-specific partial resistance of barley to leaf rust |
| topic | S Agriculture (General) SB Plant culture |
| url | http://ir.unimas.my/id/eprint/16111/ http://ir.unimas.my/id/eprint/16111/ http://ir.unimas.my/id/eprint/16111/ http://ir.unimas.my/id/eprint/16111/7/High-resolution%20mapping%20of%20genes%20involved%20in%20plant%20%28abstract%29.pdf |