Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007)
Hand Foot and Mouth Disease is a common exanthema of young children, characterized by fever, rash on the palm and bottoms of the feet and ulcers in the oral cavity. In this study, the entire region of CV A16 genome for 22 CV A16 strains associated with HFMD in Sarawak (Malaysia Borneo), isolated be...
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| Format: | Thesis |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Online Access: | http://ir.unimas.my/id/eprint/15886/ http://ir.unimas.my/id/eprint/15886/3/Katrina%20ft.pdf |
| _version_ | 1848837947458060288 |
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| author | Katrina, binti Aslan Joe |
| author_facet | Katrina, binti Aslan Joe |
| author_sort | Katrina, binti Aslan Joe |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Hand Foot and Mouth Disease is a common exanthema of young children, characterized by fever, rash on the palm and bottoms of the feet and ulcers in the oral cavity. In this study, the entire region of CV A16 genome for 22 CV A16 strains associated with HFMD in Sarawak (Malaysia Borneo), isolated between 1997 and 2008 were successfully sequenced. There were 25 sets of full CVA16 genome primers which were designed in such a way that it targets the whole genome of CVA16 regions in addition to three anti-sense gene specific primers that were used to amplify the 5'UTR region and two sense gene-specific primers that were used to amplify the 3'UTR region of the CVA16 genome. These primers successfully amplified the expected product size from extracted RNA of all 22 isolates. |
| first_indexed | 2025-11-15T06:47:45Z |
| format | Thesis |
| id | unimas-15886 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:47:45Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-158862024-08-20T06:50:03Z http://ir.unimas.my/id/eprint/15886/ Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007) Katrina, binti Aslan Joe RJ Pediatrics Hand Foot and Mouth Disease is a common exanthema of young children, characterized by fever, rash on the palm and bottoms of the feet and ulcers in the oral cavity. In this study, the entire region of CV A16 genome for 22 CV A16 strains associated with HFMD in Sarawak (Malaysia Borneo), isolated between 1997 and 2008 were successfully sequenced. There were 25 sets of full CVA16 genome primers which were designed in such a way that it targets the whole genome of CVA16 regions in addition to three anti-sense gene specific primers that were used to amplify the 5'UTR region and two sense gene-specific primers that were used to amplify the 3'UTR region of the CVA16 genome. These primers successfully amplified the expected product size from extracted RNA of all 22 isolates. Universiti Malaysia Sarawak, (UNIMAS) 2012 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/15886/3/Katrina%20ft.pdf Katrina, binti Aslan Joe (2012) Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007). Masters thesis, Universiti Malaysia Sarawak, (UNIMAS). |
| spellingShingle | RJ Pediatrics Katrina, binti Aslan Joe Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007) |
| title | Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007) |
| title_full | Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007) |
| title_fullStr | Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007) |
| title_full_unstemmed | Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007) |
| title_short | Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007) |
| title_sort | complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in sarawak (1999-2007) |
| topic | RJ Pediatrics |
| url | http://ir.unimas.my/id/eprint/15886/ http://ir.unimas.my/id/eprint/15886/3/Katrina%20ft.pdf |