Short Report: Differences In Dihydrofolate Reductase But Not Dihydropteroate Synthase Alleles In Plasmodium Falciparum Isolates From Geographically Distinct Areas In Malaysia
Dihydropteroate synthase (dhps) and dihydrofolate reductase (dhfr) alleles were typed in 67 Malaysian Plasmodium falciparum isolates. The isolates were collected from two geographically distinct locations: 51 from Sabah, Malaysian Borneo, where sulfadoxine/pyrimethamine (SDX/PYR) is used to treat...
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| Format: | Article |
| Language: | English |
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American Society of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene
2001
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| Online Access: | http://ir.unimas.my/id/eprint/15866/ http://ir.unimas.my/id/eprint/15866/1/SHORT%20REPORT%20%20DIFFERENCES%20IN%20DIHYDROFOLATE%20REDUCTASE%20%28abstract%29.pdf |
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| author | Cox-Singh, J. Balbir, Singh Robaiza, Zakaria Mohd Shukri, Abdullah Hasan, Abdul Rahman Nagappan, Sethu |
| author_facet | Cox-Singh, J. Balbir, Singh Robaiza, Zakaria Mohd Shukri, Abdullah Hasan, Abdul Rahman Nagappan, Sethu |
| author_sort | Cox-Singh, J. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Dihydropteroate synthase (dhps) and dihydrofolate reductase (dhfr) alleles were typed in 67 Malaysian
Plasmodium falciparum isolates. The isolates were collected from two geographically distinct locations: 51 from
Sabah, Malaysian Borneo, where sulfadoxine/pyrimethamine (SDX/PYR) is used to treat uncomplicated malaria and
16 from Peninsular Malaysia where in vivo resistance to SDX/PYR has been reported. A total of seven dhps alleles
were identified with no significant difference in allele frequency between the 2 populations. Two of the dhps alleles
described here have not been previously reported. Four dhfr alleles were detected in 67 P. falciparum isolates. Eightyseven
percent of the isolates from the Peninsula, where clinical SDX/PYR failure has been reported, had dhfr alleles
with triple point mutations while all of the isolates from Sabah had dhfr alleles with 2 or less point mutations. The
difference in dhfr allele frequency between the two populations was highly significant. There was no correlation
between in vitro PYR response and accumulation of dhfr point mutations. |
| first_indexed | 2025-11-15T06:47:41Z |
| format | Article |
| id | unimas-15866 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:47:41Z |
| publishDate | 2001 |
| publisher | American Society of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-158662017-04-06T07:15:08Z http://ir.unimas.my/id/eprint/15866/ Short Report: Differences In Dihydrofolate Reductase But Not Dihydropteroate Synthase Alleles In Plasmodium Falciparum Isolates From Geographically Distinct Areas In Malaysia Cox-Singh, J. Balbir, Singh Robaiza, Zakaria Mohd Shukri, Abdullah Hasan, Abdul Rahman Nagappan, Sethu Q Science (General) R Medicine (General) Dihydropteroate synthase (dhps) and dihydrofolate reductase (dhfr) alleles were typed in 67 Malaysian Plasmodium falciparum isolates. The isolates were collected from two geographically distinct locations: 51 from Sabah, Malaysian Borneo, where sulfadoxine/pyrimethamine (SDX/PYR) is used to treat uncomplicated malaria and 16 from Peninsular Malaysia where in vivo resistance to SDX/PYR has been reported. A total of seven dhps alleles were identified with no significant difference in allele frequency between the 2 populations. Two of the dhps alleles described here have not been previously reported. Four dhfr alleles were detected in 67 P. falciparum isolates. Eightyseven percent of the isolates from the Peninsula, where clinical SDX/PYR failure has been reported, had dhfr alleles with triple point mutations while all of the isolates from Sabah had dhfr alleles with 2 or less point mutations. The difference in dhfr allele frequency between the two populations was highly significant. There was no correlation between in vitro PYR response and accumulation of dhfr point mutations. American Society of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene 2001 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15866/1/SHORT%20REPORT%20%20DIFFERENCES%20IN%20DIHYDROFOLATE%20REDUCTASE%20%28abstract%29.pdf Cox-Singh, J. and Balbir, Singh and Robaiza, Zakaria and Mohd Shukri, Abdullah and Hasan, Abdul Rahman and Nagappan, Sethu (2001) Short Report: Differences In Dihydrofolate Reductase But Not Dihydropteroate Synthase Alleles In Plasmodium Falciparum Isolates From Geographically Distinct Areas In Malaysia. The American journal of tropical medicine and hygiene, 64 (1-2). pp. 28-31. ISSN 0002-9637 http://www.ajtmh.org/ |
| spellingShingle | Q Science (General) R Medicine (General) Cox-Singh, J. Balbir, Singh Robaiza, Zakaria Mohd Shukri, Abdullah Hasan, Abdul Rahman Nagappan, Sethu Short Report: Differences In Dihydrofolate Reductase But Not Dihydropteroate Synthase Alleles In Plasmodium Falciparum Isolates From Geographically Distinct Areas In Malaysia |
| title | Short Report: Differences In Dihydrofolate Reductase But Not Dihydropteroate Synthase Alleles In Plasmodium Falciparum Isolates From Geographically Distinct Areas In Malaysia |
| title_full | Short Report: Differences In Dihydrofolate Reductase But Not Dihydropteroate Synthase Alleles In Plasmodium Falciparum Isolates From Geographically Distinct Areas In Malaysia |
| title_fullStr | Short Report: Differences In Dihydrofolate Reductase But Not Dihydropteroate Synthase Alleles In Plasmodium Falciparum Isolates From Geographically Distinct Areas In Malaysia |
| title_full_unstemmed | Short Report: Differences In Dihydrofolate Reductase But Not Dihydropteroate Synthase Alleles In Plasmodium Falciparum Isolates From Geographically Distinct Areas In Malaysia |
| title_short | Short Report: Differences In Dihydrofolate Reductase But Not Dihydropteroate Synthase Alleles In Plasmodium Falciparum Isolates From Geographically Distinct Areas In Malaysia |
| title_sort | short report: differences in dihydrofolate reductase but not dihydropteroate synthase alleles in plasmodium falciparum isolates from geographically distinct areas in malaysia |
| topic | Q Science (General) R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/15866/ http://ir.unimas.my/id/eprint/15866/ http://ir.unimas.my/id/eprint/15866/1/SHORT%20REPORT%20%20DIFFERENCES%20IN%20DIHYDROFOLATE%20REDUCTASE%20%28abstract%29.pdf |