Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays
The blood filtration method was used as the gold standard to determine the detection level of simple blood-spot sampling and nested-polymerase chain reaction (PCR) for Brugia malayi. Of 100 samples, 48 were filtration-positive. Of these, 26 had microfilaria counts that were low enough (<1–29 micr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier Ltd.
2000
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/15864/ http://ir.unimas.my/id/eprint/15864/1/Sensitivity%20of%20the%20nested-polymerase%20chain%28abstact%29.pdf |
| _version_ | 1848837942533947392 |
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| author | Cox-Singh, J. Pomrehn, Andrea S. Wolfe, Nathan D. Hasan, A. Rahman Huong-Ying, Lu Balbir, Singh |
| author_facet | Cox-Singh, J. Pomrehn, Andrea S. Wolfe, Nathan D. Hasan, A. Rahman Huong-Ying, Lu Balbir, Singh |
| author_sort | Cox-Singh, J. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The blood filtration method was used as the gold standard to determine the detection level of simple blood-spot sampling and nested-polymerase chain reaction (PCR) for Brugia malayi. Of 100 samples, 48 were filtration-positive. Of these, 26 had microfilaria counts that were low enough (<1–29 microfilariae/ml) to accurately assess the limit of detection by nested-PCR. Nested-PCR consistently detected B. malayi DNA in samples with ≥10 microfilariae/ml. Post-filtration, microfilaria-depleted, blood-spots from microfilaria-positive samples were screened by nested-PCR and B. malayi specific ‘free’ DNA was detected in 51.7% of these samples. There was no evidence for ‘free’ DNA in microfilaria-negative individuals from this endemic community. |
| first_indexed | 2025-11-15T06:47:40Z |
| format | Article |
| id | unimas-15864 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:47:40Z |
| publishDate | 2000 |
| publisher | Elsevier Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-158642017-04-07T02:08:22Z http://ir.unimas.my/id/eprint/15864/ Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays Cox-Singh, J. Pomrehn, Andrea S. Wolfe, Nathan D. Hasan, A. Rahman Huong-Ying, Lu Balbir, Singh R Medicine (General) The blood filtration method was used as the gold standard to determine the detection level of simple blood-spot sampling and nested-polymerase chain reaction (PCR) for Brugia malayi. Of 100 samples, 48 were filtration-positive. Of these, 26 had microfilaria counts that were low enough (<1–29 microfilariae/ml) to accurately assess the limit of detection by nested-PCR. Nested-PCR consistently detected B. malayi DNA in samples with ≥10 microfilariae/ml. Post-filtration, microfilaria-depleted, blood-spots from microfilaria-positive samples were screened by nested-PCR and B. malayi specific ‘free’ DNA was detected in 51.7% of these samples. There was no evidence for ‘free’ DNA in microfilaria-negative individuals from this endemic community. Elsevier Ltd. 2000 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15864/1/Sensitivity%20of%20the%20nested-polymerase%20chain%28abstact%29.pdf Cox-Singh, J. and Pomrehn, Andrea S. and Wolfe, Nathan D. and Hasan, A. Rahman and Huong-Ying, Lu and Balbir, Singh (2000) Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays. International Journal for Parasitology, 30 (11). pp. 1177-1179. ISSN 0020-7519 http://www.sciencedirect.com/science/article/pii/S0020751900001041 http://doi.org/10.1016/S0020-7519(00)00104-1 |
| spellingShingle | R Medicine (General) Cox-Singh, J. Pomrehn, Andrea S. Wolfe, Nathan D. Hasan, A. Rahman Huong-Ying, Lu Balbir, Singh Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays |
| title | Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays |
| title_full | Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays |
| title_fullStr | Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays |
| title_full_unstemmed | Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays |
| title_short | Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays |
| title_sort | sensitivity of the nested-polymerase chain reaction (pcr) assay for brugia malayi and signi®cance of `free' dna in pcr-based assays |
| topic | R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/15864/ http://ir.unimas.my/id/eprint/15864/ http://ir.unimas.my/id/eprint/15864/ http://ir.unimas.my/id/eprint/15864/1/Sensitivity%20of%20the%20nested-polymerase%20chain%28abstact%29.pdf |