Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays
The blood filtration method was used as the gold standard to determine the detection level of simple blood-spot sampling and nested-polymerase chain reaction (PCR) for Brugia malayi. Of 100 samples, 48 were filtration-positive. Of these, 26 had microfilaria counts that were low enough (<1–29 micr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier Ltd.
2000
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/15864/ http://ir.unimas.my/id/eprint/15864/1/Sensitivity%20of%20the%20nested-polymerase%20chain%28abstact%29.pdf |
| Summary: | The blood filtration method was used as the gold standard to determine the detection level of simple blood-spot sampling and nested-polymerase chain reaction (PCR) for Brugia malayi. Of 100 samples, 48 were filtration-positive. Of these, 26 had microfilaria counts that were low enough (<1–29 microfilariae/ml) to accurately assess the limit of detection by nested-PCR. Nested-PCR consistently detected B. malayi DNA in samples with ≥10 microfilariae/ml. Post-filtration, microfilaria-depleted, blood-spots from microfilaria-positive samples were screened by nested-PCR and B. malayi specific ‘free’ DNA was detected in 51.7% of these samples. There was no evidence for ‘free’ DNA in microfilaria-negative individuals from this endemic community. |
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