A Genus-and Species-Specific Nested Polymerase Chain Reaction Malaria Detection Assay For Epidemiologic Studies
A nested polymerase chain reaction (PCR) assay that uses Plasmodium genus-specific primers for the initial PCR (nest 1) amplification and either genus- or species-specific primers for the nest 2 amplifications was tested on laboratory and field samples. With in vitro cultured Plasmodium falciparum–...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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American Society of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene
1999
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| Online Access: | http://ir.unimas.my/id/eprint/15838/ http://ir.unimas.my/id/eprint/15838/1/A%20GENUS.pdf |
| _version_ | 1848837936570695680 |
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| author | Balbir, Singh Bobogare, Albino Cox-Singh, J. Snounou, Georges Mohammad Shukri, Abdullah Hasan, Abdul Rahman |
| author_facet | Balbir, Singh Bobogare, Albino Cox-Singh, J. Snounou, Georges Mohammad Shukri, Abdullah Hasan, Abdul Rahman |
| author_sort | Balbir, Singh |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A nested polymerase chain reaction (PCR) assay that uses Plasmodium genus-specific primers for the
initial PCR (nest 1) amplification and either genus- or species-specific primers for the nest 2 amplifications was tested on laboratory and field samples. With in vitro cultured Plasmodium falciparum–infected blood samples, it was capable of detecting six parasites/ml of blood using DNA prepared from 25-ml blood spots on filter paper. The assay was evaluated on fingerprick blood samples collected on filter paper from 129 individuals living in a malaria-endemic area in Malaysia. Malaria prevalence by genus-specific nested PCR was 35.6% (46 of 129) compared with 28.7% (37 of 129) by microscopy. The nested PCR detected seven more malaria samples than microscopy in the first round of microscopic examination, malaria in three microscopically negative samples, six double infections identified as single infections by microscopy and one triple infection identified as a double infection by microscopy. The nested PCR assay described is a sensitive technique for collecting accurate malaria epidemiologic data. When coupled with
simple blood spot sampling, it is particularly useful for screening communities in remote regions of the world. |
| first_indexed | 2025-11-15T06:47:35Z |
| format | Article |
| id | unimas-15838 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:47:35Z |
| publishDate | 1999 |
| publisher | American Society of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-158382022-01-19T01:22:47Z http://ir.unimas.my/id/eprint/15838/ A Genus-and Species-Specific Nested Polymerase Chain Reaction Malaria Detection Assay For Epidemiologic Studies Balbir, Singh Bobogare, Albino Cox-Singh, J. Snounou, Georges Mohammad Shukri, Abdullah Hasan, Abdul Rahman R Medicine (General) A nested polymerase chain reaction (PCR) assay that uses Plasmodium genus-specific primers for the initial PCR (nest 1) amplification and either genus- or species-specific primers for the nest 2 amplifications was tested on laboratory and field samples. With in vitro cultured Plasmodium falciparum–infected blood samples, it was capable of detecting six parasites/ml of blood using DNA prepared from 25-ml blood spots on filter paper. The assay was evaluated on fingerprick blood samples collected on filter paper from 129 individuals living in a malaria-endemic area in Malaysia. Malaria prevalence by genus-specific nested PCR was 35.6% (46 of 129) compared with 28.7% (37 of 129) by microscopy. The nested PCR detected seven more malaria samples than microscopy in the first round of microscopic examination, malaria in three microscopically negative samples, six double infections identified as single infections by microscopy and one triple infection identified as a double infection by microscopy. The nested PCR assay described is a sensitive technique for collecting accurate malaria epidemiologic data. When coupled with simple blood spot sampling, it is particularly useful for screening communities in remote regions of the world. American Society of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene 1999 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15838/1/A%20GENUS.pdf Balbir, Singh and Bobogare, Albino and Cox-Singh, J. and Snounou, Georges and Mohammad Shukri, Abdullah and Hasan, Abdul Rahman (1999) A Genus-and Species-Specific Nested Polymerase Chain Reaction Malaria Detection Assay For Epidemiologic Studies. The American journal of tropical medicine and hygiene, 60 (4). pp. 687-692. ISSN 0002-9637 https://www.researchgate.net/publication/12951123_A_Genus-_and_species-specific_nested_polymerase_chain_reaction_malaria_detection_assay_for_epidemiologic_studies |
| spellingShingle | R Medicine (General) Balbir, Singh Bobogare, Albino Cox-Singh, J. Snounou, Georges Mohammad Shukri, Abdullah Hasan, Abdul Rahman A Genus-and Species-Specific Nested Polymerase Chain Reaction Malaria Detection Assay For Epidemiologic Studies |
| title | A Genus-and Species-Specific Nested Polymerase Chain Reaction Malaria Detection Assay For Epidemiologic Studies |
| title_full | A Genus-and Species-Specific Nested Polymerase Chain Reaction Malaria Detection Assay For Epidemiologic Studies |
| title_fullStr | A Genus-and Species-Specific Nested Polymerase Chain Reaction Malaria Detection Assay For Epidemiologic Studies |
| title_full_unstemmed | A Genus-and Species-Specific Nested Polymerase Chain Reaction Malaria Detection Assay For Epidemiologic Studies |
| title_short | A Genus-and Species-Specific Nested Polymerase Chain Reaction Malaria Detection Assay For Epidemiologic Studies |
| title_sort | genus-and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies |
| topic | R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/15838/ http://ir.unimas.my/id/eprint/15838/ http://ir.unimas.my/id/eprint/15838/1/A%20GENUS.pdf |