Molecular Epidemiological Investigation of Plasmodium knowlesi in Humans and Macaques in Singapore
Singapore reported its first locally acquired human Plasmodium knowlesi infection in 2007, involving a soldier who had undergone training in a forested area where long-tailed macaques are frequently seen. Comprehensive disease surveillance and monitoring system that was set up after the initial ca...
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| Format: | Article |
| Language: | English |
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Mary Ann Liebert, Inc.
2011
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| Online Access: | http://ir.unimas.my/id/eprint/15810/ http://ir.unimas.my/id/eprint/15810/1/Molecular%20Epidemiological%20Investigation%20of%20Plasmodium%20%28abstract%29.pdf |
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| author | Wong, Pei Sze Jeslyn Tan, Cheong Huat Lee, Vernon Li, Mei Zhi Irene Lee, Kim Sung Lee, Piao Jarrod Balbir, Singh Ng, Lee Ching |
| author_facet | Wong, Pei Sze Jeslyn Tan, Cheong Huat Lee, Vernon Li, Mei Zhi Irene Lee, Kim Sung Lee, Piao Jarrod Balbir, Singh Ng, Lee Ching |
| author_sort | Wong, Pei Sze Jeslyn |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Singapore reported its first locally acquired human Plasmodium knowlesi infection in 2007, involving a soldier
who had undergone training in a forested area where long-tailed macaques are frequently seen. Comprehensive
disease surveillance and monitoring system that was set up after the initial case detected four additional human
P. knowlesi cases in 2007 and one in 2008. All involved military personnel who had undergone training in the
forested area, and none had traveled out of Singapore 1 month before the onset of symptoms. Screening for
malaria parasites on blood obtained from long-tailed macaques revealed that wild monkeys (n¼3) caught from
the forested area were infected with P. knowlesi, whereas peri-domestic monkeys (n¼10) caught from a nature
reserve park were not infected with any malaria parasites. Phylogenetic analysis of the nonrepeat region of the
P. knowlesi csp genes showed that the sequences obtained from the human cases were not distinct from those
obtained from wild monkeys. Further, certain genotypes were shared between samples from humans and
macaques. Our findings provide evidence that long-tailed macaques are the natural hosts of P. knowlesi in
Singapore and the human cases acquired their infection in the same vicinity where these monkeys are found.
Further, the risk of acquiring P. knowlesi infection among the general population of Singapore is small as evident from the absence of P. knowlesi in peri-domestic monkeys. |
| first_indexed | 2025-11-15T06:47:30Z |
| format | Article |
| id | unimas-15810 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:47:30Z |
| publishDate | 2011 |
| publisher | Mary Ann Liebert, Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-158102017-04-04T06:31:37Z http://ir.unimas.my/id/eprint/15810/ Molecular Epidemiological Investigation of Plasmodium knowlesi in Humans and Macaques in Singapore Wong, Pei Sze Jeslyn Tan, Cheong Huat Lee, Vernon Li, Mei Zhi Irene Lee, Kim Sung Lee, Piao Jarrod Balbir, Singh Ng, Lee Ching R Medicine (General) Singapore reported its first locally acquired human Plasmodium knowlesi infection in 2007, involving a soldier who had undergone training in a forested area where long-tailed macaques are frequently seen. Comprehensive disease surveillance and monitoring system that was set up after the initial case detected four additional human P. knowlesi cases in 2007 and one in 2008. All involved military personnel who had undergone training in the forested area, and none had traveled out of Singapore 1 month before the onset of symptoms. Screening for malaria parasites on blood obtained from long-tailed macaques revealed that wild monkeys (n¼3) caught from the forested area were infected with P. knowlesi, whereas peri-domestic monkeys (n¼10) caught from a nature reserve park were not infected with any malaria parasites. Phylogenetic analysis of the nonrepeat region of the P. knowlesi csp genes showed that the sequences obtained from the human cases were not distinct from those obtained from wild monkeys. Further, certain genotypes were shared between samples from humans and macaques. Our findings provide evidence that long-tailed macaques are the natural hosts of P. knowlesi in Singapore and the human cases acquired their infection in the same vicinity where these monkeys are found. Further, the risk of acquiring P. knowlesi infection among the general population of Singapore is small as evident from the absence of P. knowlesi in peri-domestic monkeys. Mary Ann Liebert, Inc. 2011 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15810/1/Molecular%20Epidemiological%20Investigation%20of%20Plasmodium%20%28abstract%29.pdf Wong, Pei Sze Jeslyn and Tan, Cheong Huat and Lee, Vernon and Li, Mei Zhi Irene and Lee, Kim Sung and Lee, Piao Jarrod and Balbir, Singh and Ng, Lee Ching (2011) Molecular Epidemiological Investigation of Plasmodium knowlesi in Humans and Macaques in Singapore. Vector borne and zoonotic diseases, 11 (2). ISSN 1557-7759 https://www.researchgate.net/publication/44850761 DOI: 10.1089/vbz.2010.0024 |
| spellingShingle | R Medicine (General) Wong, Pei Sze Jeslyn Tan, Cheong Huat Lee, Vernon Li, Mei Zhi Irene Lee, Kim Sung Lee, Piao Jarrod Balbir, Singh Ng, Lee Ching Molecular Epidemiological Investigation of Plasmodium knowlesi in Humans and Macaques in Singapore |
| title | Molecular Epidemiological Investigation of Plasmodium
knowlesi in Humans and Macaques in Singapore |
| title_full | Molecular Epidemiological Investigation of Plasmodium
knowlesi in Humans and Macaques in Singapore |
| title_fullStr | Molecular Epidemiological Investigation of Plasmodium
knowlesi in Humans and Macaques in Singapore |
| title_full_unstemmed | Molecular Epidemiological Investigation of Plasmodium
knowlesi in Humans and Macaques in Singapore |
| title_short | Molecular Epidemiological Investigation of Plasmodium
knowlesi in Humans and Macaques in Singapore |
| title_sort | molecular epidemiological investigation of plasmodium
knowlesi in humans and macaques in singapore |
| topic | R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/15810/ http://ir.unimas.my/id/eprint/15810/ http://ir.unimas.my/id/eprint/15810/ http://ir.unimas.my/id/eprint/15810/1/Molecular%20Epidemiological%20Investigation%20of%20Plasmodium%20%28abstract%29.pdf |