Laboratory efficacy of mycoparasitic fungi (Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs (Cimex hemipterus) (Hemiptera: Cimicidae)

Objective: To test the effectiveness of conidial spore formulations [Aspergillus tubingensis (A. tubingensis) and Trichoderma harzianum (T. harzianum)] against tropical bed bugs, Cimex hemipterus. Methods: Spore formulations were made from two fungal strains, T. harzianum and A. tubingensis. The...

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Main Authors: Zulaikha, Zahran, Nik Mohd Izham, Mohamed Nor, Hamady, Dieng, Tomomitsu, Satho, Abdul Hafiz, Ab Majid
Format: Article
Language:English
Published: Elsevier 2017
Subjects:
Online Access:http://ir.unimas.my/id/eprint/15638/
http://ir.unimas.my/id/eprint/15638/7/zahran.pdf
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author Zulaikha, Zahran
Nik Mohd Izham, Mohamed Nor
Hamady, Dieng
Tomomitsu, Satho
Abdul Hafiz, Ab Majid
author_facet Zulaikha, Zahran
Nik Mohd Izham, Mohamed Nor
Hamady, Dieng
Tomomitsu, Satho
Abdul Hafiz, Ab Majid
author_sort Zulaikha, Zahran
building UNIMAS Institutional Repository
collection Online Access
description Objective: To test the effectiveness of conidial spore formulations [Aspergillus tubingensis (A. tubingensis) and Trichoderma harzianum (T. harzianum)] against tropical bed bugs, Cimex hemipterus. Methods: Spore formulations were made from two fungal strains, T. harzianum and A. tubingensis. The bed bugs were exposed to the conidial spores placed soaked onto a fabric cloth for 1 h and the mortality counts were recorded daily until 14 days. Results: Mean survival times based on Kaplan–Meier survival analysis showed no significant differences between all the concentrations in both the fungal isolates: T. harzianum and A. tubingensis. However, the evaluation of both the isolates in terms of virulence resulted in low lethal hours in all the concentrations except for the high concentration of A. tubingensis (LT50 = 44.629 h) at the conidial exposure of 1 × 106 spores/ mL. Rapid mortality of the bed bugs was observed from Day 6 to Day 12, ranging from 13% to 90% in all three concentrations of A. tubingensis. With reference to the T. harzianum exposure, the concentration of 1 × 104 spores/mL displayed a gradual increase in the percentage mortality of 90 on Day 14. Conclusions: Approaches to the bed bugs treatment should be explored in-depth using a natural biological agent like fungus especially A. tubingensis to reduce this pest population, in order to replace chemical methods.
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spelling unimas-156382023-03-31T08:00:18Z http://ir.unimas.my/id/eprint/15638/ Laboratory efficacy of mycoparasitic fungi (Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs (Cimex hemipterus) (Hemiptera: Cimicidae) Zulaikha, Zahran Nik Mohd Izham, Mohamed Nor Hamady, Dieng Tomomitsu, Satho Abdul Hafiz, Ab Majid GE Environmental Sciences Objective: To test the effectiveness of conidial spore formulations [Aspergillus tubingensis (A. tubingensis) and Trichoderma harzianum (T. harzianum)] against tropical bed bugs, Cimex hemipterus. Methods: Spore formulations were made from two fungal strains, T. harzianum and A. tubingensis. The bed bugs were exposed to the conidial spores placed soaked onto a fabric cloth for 1 h and the mortality counts were recorded daily until 14 days. Results: Mean survival times based on Kaplan–Meier survival analysis showed no significant differences between all the concentrations in both the fungal isolates: T. harzianum and A. tubingensis. However, the evaluation of both the isolates in terms of virulence resulted in low lethal hours in all the concentrations except for the high concentration of A. tubingensis (LT50 = 44.629 h) at the conidial exposure of 1 × 106 spores/ mL. Rapid mortality of the bed bugs was observed from Day 6 to Day 12, ranging from 13% to 90% in all three concentrations of A. tubingensis. With reference to the T. harzianum exposure, the concentration of 1 × 104 spores/mL displayed a gradual increase in the percentage mortality of 90 on Day 14. Conclusions: Approaches to the bed bugs treatment should be explored in-depth using a natural biological agent like fungus especially A. tubingensis to reduce this pest population, in order to replace chemical methods. Elsevier 2017 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15638/7/zahran.pdf Zulaikha, Zahran and Nik Mohd Izham, Mohamed Nor and Hamady, Dieng and Tomomitsu, Satho and Abdul Hafiz, Ab Majid (2017) Laboratory efficacy of mycoparasitic fungi (Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs (Cimex hemipterus) (Hemiptera: Cimicidae). Asian Pacific Journal of Tropical Biomedicine, 7 (4). pp. 288-293. ISSN 22211691 http://www.sciencedirect.com/science/article/pii/S2221169117300394 DOI: 10.1016/j.apjtb.2016.12.021
spellingShingle GE Environmental Sciences
Zulaikha, Zahran
Nik Mohd Izham, Mohamed Nor
Hamady, Dieng
Tomomitsu, Satho
Abdul Hafiz, Ab Majid
Laboratory efficacy of mycoparasitic fungi (Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs (Cimex hemipterus) (Hemiptera: Cimicidae)
title Laboratory efficacy of mycoparasitic fungi (Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs (Cimex hemipterus) (Hemiptera: Cimicidae)
title_full Laboratory efficacy of mycoparasitic fungi (Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs (Cimex hemipterus) (Hemiptera: Cimicidae)
title_fullStr Laboratory efficacy of mycoparasitic fungi (Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs (Cimex hemipterus) (Hemiptera: Cimicidae)
title_full_unstemmed Laboratory efficacy of mycoparasitic fungi (Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs (Cimex hemipterus) (Hemiptera: Cimicidae)
title_short Laboratory efficacy of mycoparasitic fungi (Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs (Cimex hemipterus) (Hemiptera: Cimicidae)
title_sort laboratory efficacy of mycoparasitic fungi (aspergillus tubingensis and trichoderma harzianum) against tropical bed bugs (cimex hemipterus) (hemiptera: cimicidae)
topic GE Environmental Sciences
url http://ir.unimas.my/id/eprint/15638/
http://ir.unimas.my/id/eprint/15638/
http://ir.unimas.my/id/eprint/15638/
http://ir.unimas.my/id/eprint/15638/7/zahran.pdf