Ancestry, Plasmodium cynomolgi prevalence and rhesus macaque admixture in cynomolgus macaques (Macaca fascicularis) bred for export in Chinese breeding farms
Background: Most cynomolgus macaques (Macaca fascicularis) used in the United States as animal models are imported from Chinese breeding farms without documented ancestry. Cynomolgus macaques with varying rhesus macaque ancestry proportions may exhibit differences, such as susceptibility to malar...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Blackwell Publishing Ltd
2017
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/15630/ http://ir.unimas.my/id/eprint/15630/1/Ancestry%2C%20Plasmodium%20cynomolgi%20prevalence%20%28abstract%29.pdf |
| Summary: | Background: Most cynomolgus macaques (Macaca fascicularis) used in the United
States as animal models are imported from Chinese breeding farms without documented
ancestry. Cynomolgus macaques with varying rhesus macaque ancestry proportions
may exhibit differences, such as susceptibility to malaria, that affect their
suitability as a research model.
Methods: DNA of 400 cynomolgus macaques from 10 Chinese breeding farms was
genotyped to characterize their regional origin and rhesus ancestry proportion. A
nested PCR assay was used to detect Plasmodium cynomolgi infection in sampled
individuals.
Results: All populations exhibited high levels of genetic heterogeneity and low levels
of inbreeding and genetic subdivision. Almost all individuals exhibited an Indochinese
origin and a rhesus ancestry proportion of 5%-48%.
The incidence of P. cynomolgi
infection
in cynomolgus macaques is strongly associated with proportion of rhesus
ancestry.
Conclusions: The varying amount of rhesus ancestry in cynomolgus macaques underscores
the importance of monitoring their genetic similarity in malaria research. |
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