Study of paralytic shellfish toxin and tetrodotoxin in horseshoe crabs
In this study, toxin analysis was assessed for paralytic shellfish toxin (PST) and tetrodotoxin (TTX) in horseshoe crabs at 3 sampling sites, 2 sites in Sarawak (Kuching and Samarahan) and 1 site in Johor (Kuala Sedili). For PST, toxin used for this study were saxitoxin (STX). The objectives for t...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2016
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/15469/ http://ir.unimas.my/id/eprint/15469/1/Nurulhuda%20ft.pdf |
| _version_ | 1848837860616044544 |
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| author | Nurulhuda, Hashim |
| author_facet | Nurulhuda, Hashim |
| author_sort | Nurulhuda, Hashim |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | In this study, toxin analysis was assessed for paralytic shellfish toxin (PST) and tetrodotoxin (TTX) in
horseshoe crabs at 3 sampling sites, 2 sites in Sarawak (Kuching and Samarahan) and 1 site in Johor (Kuala
Sedili). For PST, toxin used for this study were saxitoxin (STX). The objectives for this study were to study and
determine the toxin properties in horseshoe crab. There were total of 37 samples for PST and 32 samples for
TTX were tested for toxin analysis. Two species of horseshoe crabs were identified as Tachypleus gigas and
Carcinoscorpius rotundicauda and the total length, body weight, carapace width, and telson length were
measured. For toxin analysis, thin layer chromatography (TLC), high-performance liquid chromatography
(HPLC) and liquid chromatography tandem mass spectrometry (LC-MS/MS) were performed. TLC analysis for
both STX and TTX indicated no toxins presence in the horseshoe crabs. The standard STX is 0.55 and for TTX
is 0.35. Average values for STX were 0.49-0.57 and for TTX were 0.45-0.67. PST profile for HPLC indicated
no STX detected at peak compared with standard but there were other toxin presence in low concentration. For
LC-MS/MS analysis, Kuching samples was non-toxic (1.3 MU/g) and egg samples at Johor was moderately
toxic (295.27 MU/g). These findings show that horseshoe crabs at Malaysia are still considered safe for human
consumption but further research must be continued. |
| first_indexed | 2025-11-15T06:46:22Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-15469 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:46:22Z |
| publishDate | 2016 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-154692024-02-09T07:26:19Z http://ir.unimas.my/id/eprint/15469/ Study of paralytic shellfish toxin and tetrodotoxin in horseshoe crabs Nurulhuda, Hashim SH Aquaculture. Fisheries. Angling In this study, toxin analysis was assessed for paralytic shellfish toxin (PST) and tetrodotoxin (TTX) in horseshoe crabs at 3 sampling sites, 2 sites in Sarawak (Kuching and Samarahan) and 1 site in Johor (Kuala Sedili). For PST, toxin used for this study were saxitoxin (STX). The objectives for this study were to study and determine the toxin properties in horseshoe crab. There were total of 37 samples for PST and 32 samples for TTX were tested for toxin analysis. Two species of horseshoe crabs were identified as Tachypleus gigas and Carcinoscorpius rotundicauda and the total length, body weight, carapace width, and telson length were measured. For toxin analysis, thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) and liquid chromatography tandem mass spectrometry (LC-MS/MS) were performed. TLC analysis for both STX and TTX indicated no toxins presence in the horseshoe crabs. The standard STX is 0.55 and for TTX is 0.35. Average values for STX were 0.49-0.57 and for TTX were 0.45-0.67. PST profile for HPLC indicated no STX detected at peak compared with standard but there were other toxin presence in low concentration. For LC-MS/MS analysis, Kuching samples was non-toxic (1.3 MU/g) and egg samples at Johor was moderately toxic (295.27 MU/g). These findings show that horseshoe crabs at Malaysia are still considered safe for human consumption but further research must be continued. Universiti Malaysia Sarawak, (UNIMAS) 2016 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/15469/1/Nurulhuda%20ft.pdf Nurulhuda, Hashim (2016) Study of paralytic shellfish toxin and tetrodotoxin in horseshoe crabs. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | SH Aquaculture. Fisheries. Angling Nurulhuda, Hashim Study of paralytic shellfish toxin and tetrodotoxin in horseshoe crabs |
| title | Study of paralytic shellfish toxin and tetrodotoxin in horseshoe crabs |
| title_full | Study of paralytic shellfish toxin and tetrodotoxin in horseshoe crabs |
| title_fullStr | Study of paralytic shellfish toxin and tetrodotoxin in horseshoe crabs |
| title_full_unstemmed | Study of paralytic shellfish toxin and tetrodotoxin in horseshoe crabs |
| title_short | Study of paralytic shellfish toxin and tetrodotoxin in horseshoe crabs |
| title_sort | study of paralytic shellfish toxin and tetrodotoxin in horseshoe crabs |
| topic | SH Aquaculture. Fisheries. Angling |
| url | http://ir.unimas.my/id/eprint/15469/ http://ir.unimas.my/id/eprint/15469/1/Nurulhuda%20ft.pdf |