Genotyping of intermediate and saprophytic leptospira strains isolates from rats, soil and water in Sarawak using box-PCR
Leptospirosis is a globally re-emerging disease which can result to morbidity and mortality in both humans and animals. Leptospirosis is not new in Malaysia, yet the information and knowledge on this disease is still inadequate. This study aimed at genotyping intermediate and saprophytic Leptospi...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2016
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/15458/ http://ir.unimas.my/id/eprint/15458/3/Ambra%20Mera%20Anak%20Akang%2024pgs.pdf http://ir.unimas.my/id/eprint/15458/6/Ambra%20Mera%20Anak%20Akang%20ft.pdf |
| _version_ | 1848837857567834112 |
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| author | Ambra Mera, anak Akang |
| author_facet | Ambra Mera, anak Akang |
| author_sort | Ambra Mera, anak Akang |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Leptospirosis is a globally re-emerging disease which can result to morbidity and mortality
in both humans and animals. Leptospirosis is not new in Malaysia, yet the information and
knowledge on this disease is still inadequate. This study aimed at genotyping intermediate
and saprophytic Leptospira strains isolated from soil, water and rats in Sarawak by using
BOX-PCR technique and to determine the genetic relatedness among intermediate and
saprophytic Leptospira strains respectively. BOXAIR primer was able to produce BOXPCR
banding profiles with different fingerprint pattern among each of isolates. A
dendrogram was constructed by using Dice coefficient similarity matrix and UPGMA
based on fingerprint pattern obtained from the BOX-PCR profile. The phylogenetic
analysis using PyElph software has characterized 29 intermediate isolates into 9 major
clusters and 5 single isolates with calculated discriminatory index of 0.9434. Meanwhile,
for the 41 saprophytic strains, they were clustered into 10 major clusters with 5 single
isolates at the calculated discriminatory index of 0.932. The result of this study indicates
that BOX-PCR can be used to study the genetic diversity and relatedness among the
Leptospira strains based on the source and the location of isolation. |
| first_indexed | 2025-11-15T06:46:19Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-15458 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:46:19Z |
| publishDate | 2016 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-154582023-02-10T09:20:38Z http://ir.unimas.my/id/eprint/15458/ Genotyping of intermediate and saprophytic leptospira strains isolates from rats, soil and water in Sarawak using box-PCR Ambra Mera, anak Akang QR Microbiology Leptospirosis is a globally re-emerging disease which can result to morbidity and mortality in both humans and animals. Leptospirosis is not new in Malaysia, yet the information and knowledge on this disease is still inadequate. This study aimed at genotyping intermediate and saprophytic Leptospira strains isolated from soil, water and rats in Sarawak by using BOX-PCR technique and to determine the genetic relatedness among intermediate and saprophytic Leptospira strains respectively. BOXAIR primer was able to produce BOXPCR banding profiles with different fingerprint pattern among each of isolates. A dendrogram was constructed by using Dice coefficient similarity matrix and UPGMA based on fingerprint pattern obtained from the BOX-PCR profile. The phylogenetic analysis using PyElph software has characterized 29 intermediate isolates into 9 major clusters and 5 single isolates with calculated discriminatory index of 0.9434. Meanwhile, for the 41 saprophytic strains, they were clustered into 10 major clusters with 5 single isolates at the calculated discriminatory index of 0.932. The result of this study indicates that BOX-PCR can be used to study the genetic diversity and relatedness among the Leptospira strains based on the source and the location of isolation. Universiti Malaysia Sarawak, (UNIMAS) 2016 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/15458/3/Ambra%20Mera%20Anak%20Akang%2024pgs.pdf text en http://ir.unimas.my/id/eprint/15458/6/Ambra%20Mera%20Anak%20Akang%20ft.pdf Ambra Mera, anak Akang (2016) Genotyping of intermediate and saprophytic leptospira strains isolates from rats, soil and water in Sarawak using box-PCR. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QR Microbiology Ambra Mera, anak Akang Genotyping of intermediate and saprophytic leptospira strains isolates from rats, soil and water in Sarawak using box-PCR |
| title | Genotyping of intermediate and saprophytic leptospira strains isolates from rats, soil and water in Sarawak using box-PCR |
| title_full | Genotyping of intermediate and saprophytic leptospira strains isolates from rats, soil and water in Sarawak using box-PCR |
| title_fullStr | Genotyping of intermediate and saprophytic leptospira strains isolates from rats, soil and water in Sarawak using box-PCR |
| title_full_unstemmed | Genotyping of intermediate and saprophytic leptospira strains isolates from rats, soil and water in Sarawak using box-PCR |
| title_short | Genotyping of intermediate and saprophytic leptospira strains isolates from rats, soil and water in Sarawak using box-PCR |
| title_sort | genotyping of intermediate and saprophytic leptospira strains isolates from rats, soil and water in sarawak using box-pcr |
| topic | QR Microbiology |
| url | http://ir.unimas.my/id/eprint/15458/ http://ir.unimas.my/id/eprint/15458/3/Ambra%20Mera%20Anak%20Akang%2024pgs.pdf http://ir.unimas.my/id/eprint/15458/6/Ambra%20Mera%20Anak%20Akang%20ft.pdf |