Effects of yeast extract and peptone on hydrolysed sago stratch for bioethanol production

The subject of this study was the effect of yeast extract and peptone on production of glucose and ethanol productivity from hydrolysed sago starch (HSS) (100 g/L) using Saccharomyces cerevisiae. Since there are none nitrogen content was observed in sago starch, thus supplementation of nitrogen so...

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Main Author: Nur Hanisah, Amran
Format: Final Year Project Report / IMRAD
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2016
Subjects:
Online Access:http://ir.unimas.my/id/eprint/15453/
http://ir.unimas.my/id/eprint/15453/1/Nur%20Hanisah.pdf
http://ir.unimas.my/id/eprint/15453/4/Nur%20Hanisah.pdf
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author Nur Hanisah, Amran
author_facet Nur Hanisah, Amran
author_sort Nur Hanisah, Amran
building UNIMAS Institutional Repository
collection Online Access
description The subject of this study was the effect of yeast extract and peptone on production of glucose and ethanol productivity from hydrolysed sago starch (HSS) (100 g/L) using Saccharomyces cerevisiae. Since there are none nitrogen content was observed in sago starch, thus supplementation of nitrogen sources in the media are important. The method used was batch fermentation HSS with 100 mL initial volume with each was supplemented with nitrogen sources (yeast extract and peptone) and was maintained at room temperature (30 'C ± 0.5). The initial pH was set around 5.5-5.6 and fermentation was conducted in incubator shaker for 24 hours. Commercial glucose was used as control sample to compare it with HSS. In comparison of HSS with control sample, the gradual increase in dry cell weight of yeast can be observed in both fermentation media when supplemented with nitrogen sources. Based on the result from the experiment, yeast extract was proven to be the best nitrogen sources for ethanol'fermentation due to high amount of ethanol produced (54.73 g/L) compared with peptone (14.27 g/L). Thus, the supplementation of yeast extract is important to achieve fermentation efficiency (99.24%) in fermentation of ethanol using HSS
first_indexed 2025-11-15T06:46:18Z
format Final Year Project Report / IMRAD
id unimas-15453
institution Universiti Malaysia Sarawak
institution_category Local University
language English
English
last_indexed 2025-11-15T06:46:18Z
publishDate 2016
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-154532024-02-09T04:56:34Z http://ir.unimas.my/id/eprint/15453/ Effects of yeast extract and peptone on hydrolysed sago stratch for bioethanol production Nur Hanisah, Amran S Agriculture (General) The subject of this study was the effect of yeast extract and peptone on production of glucose and ethanol productivity from hydrolysed sago starch (HSS) (100 g/L) using Saccharomyces cerevisiae. Since there are none nitrogen content was observed in sago starch, thus supplementation of nitrogen sources in the media are important. The method used was batch fermentation HSS with 100 mL initial volume with each was supplemented with nitrogen sources (yeast extract and peptone) and was maintained at room temperature (30 'C ± 0.5). The initial pH was set around 5.5-5.6 and fermentation was conducted in incubator shaker for 24 hours. Commercial glucose was used as control sample to compare it with HSS. In comparison of HSS with control sample, the gradual increase in dry cell weight of yeast can be observed in both fermentation media when supplemented with nitrogen sources. Based on the result from the experiment, yeast extract was proven to be the best nitrogen sources for ethanol'fermentation due to high amount of ethanol produced (54.73 g/L) compared with peptone (14.27 g/L). Thus, the supplementation of yeast extract is important to achieve fermentation efficiency (99.24%) in fermentation of ethanol using HSS Universiti Malaysia Sarawak, (UNIMAS) 2016 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/15453/1/Nur%20Hanisah.pdf text en http://ir.unimas.my/id/eprint/15453/4/Nur%20Hanisah.pdf Nur Hanisah, Amran (2016) Effects of yeast extract and peptone on hydrolysed sago stratch for bioethanol production. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle S Agriculture (General)
Nur Hanisah, Amran
Effects of yeast extract and peptone on hydrolysed sago stratch for bioethanol production
title Effects of yeast extract and peptone on hydrolysed sago stratch for bioethanol production
title_full Effects of yeast extract and peptone on hydrolysed sago stratch for bioethanol production
title_fullStr Effects of yeast extract and peptone on hydrolysed sago stratch for bioethanol production
title_full_unstemmed Effects of yeast extract and peptone on hydrolysed sago stratch for bioethanol production
title_short Effects of yeast extract and peptone on hydrolysed sago stratch for bioethanol production
title_sort effects of yeast extract and peptone on hydrolysed sago stratch for bioethanol production
topic S Agriculture (General)
url http://ir.unimas.my/id/eprint/15453/
http://ir.unimas.my/id/eprint/15453/1/Nur%20Hanisah.pdf
http://ir.unimas.my/id/eprint/15453/4/Nur%20Hanisah.pdf