Decolourisation Capabilities of Ligninolytic Enzymes Produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes
Marasmius cladophyllus was examined for its ability to degradatively decolourise the recalcitrant dye Remazol Brilliant Blue R (RBBR) and screened for the production of ligninolytic enzymes using specific substrates. Monitoring dye decolourisation by the decrease in absorbance ratio of A 592/A 500 s...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2017
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| Online Access: | http://ir.unimas.my/id/eprint/15424/ http://ir.unimas.my/id/eprint/15424/7/Decolourisation%20Capabilities%20of%20Ligninolytic%20Enzymes%20Produced%20%28abstract%29.pdf |
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| author | Ngieng, Ngui Sing Ahmad, Husaini Azham, Zulkharnain Hairul Azman, Roslan |
| author_facet | Ngieng, Ngui Sing Ahmad, Husaini Azham, Zulkharnain Hairul Azman, Roslan |
| author_sort | Ngieng, Ngui Sing |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Marasmius cladophyllus was examined for its ability to degradatively decolourise the recalcitrant dye Remazol Brilliant Blue R (RBBR) and screened for the production of ligninolytic enzymes using specific substrates. Monitoring dye decolourisation by the decrease in absorbance ratio of A 592/A 500 shows that the decolourisation of RBBR dye was associated with the dye degradation. Marasmius cladophyllus produces laccase and lignin peroxidase in glucose minimal liquid medium containing RBBR. Both enzyme activities were increased, with laccase activity recorded 70 times higher reaching up to 390 U L-1 on day 12. Further in vitro RBBR dye decolourisation using the culture medium shows that laccase activity was correlated with the dye decolourisation. Fresh RBBR dye continuously supplemented into the decolourised culture medium was further decolourised much faster in the subsequent round of the RBBR dye decolourisation. In vitro dye decolourisation using the crude laccase not only decolourised 76% of RBBR dye in just 19 hours but also decolourised 54% of Orange G and 33% of Congo red at the same period of time without the use of any exogenous mediator. This rapid dye decolourisation ability of the enzymes produced by M. cladophyllus thus suggested its possible application in the bioremediation of dye containing wastewater |
| first_indexed | 2025-11-15T06:46:12Z |
| format | Article |
| id | unimas-15424 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:46:12Z |
| publishDate | 2017 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
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| spelling | unimas-154242017-02-28T06:38:32Z http://ir.unimas.my/id/eprint/15424/ Decolourisation Capabilities of Ligninolytic Enzymes Produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes Ngieng, Ngui Sing Ahmad, Husaini Azham, Zulkharnain Hairul Azman, Roslan GE Environmental Sciences Marasmius cladophyllus was examined for its ability to degradatively decolourise the recalcitrant dye Remazol Brilliant Blue R (RBBR) and screened for the production of ligninolytic enzymes using specific substrates. Monitoring dye decolourisation by the decrease in absorbance ratio of A 592/A 500 shows that the decolourisation of RBBR dye was associated with the dye degradation. Marasmius cladophyllus produces laccase and lignin peroxidase in glucose minimal liquid medium containing RBBR. Both enzyme activities were increased, with laccase activity recorded 70 times higher reaching up to 390 U L-1 on day 12. Further in vitro RBBR dye decolourisation using the culture medium shows that laccase activity was correlated with the dye decolourisation. Fresh RBBR dye continuously supplemented into the decolourised culture medium was further decolourised much faster in the subsequent round of the RBBR dye decolourisation. In vitro dye decolourisation using the crude laccase not only decolourised 76% of RBBR dye in just 19 hours but also decolourised 54% of Orange G and 33% of Congo red at the same period of time without the use of any exogenous mediator. This rapid dye decolourisation ability of the enzymes produced by M. cladophyllus thus suggested its possible application in the bioremediation of dye containing wastewater Hindawi Publishing Corporation 2017 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15424/7/Decolourisation%20Capabilities%20of%20Ligninolytic%20Enzymes%20Produced%20%28abstract%29.pdf Ngieng, Ngui Sing and Ahmad, Husaini and Azham, Zulkharnain and Hairul Azman, Roslan (2017) Decolourisation Capabilities of Ligninolytic Enzymes Produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes. BioMed Research International. ISSN 23146133 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010664469&doi=10.1155%2f2017%2f1325754&partnerID=40&md5=8604124a849197810896a3b29ccd70cb DOI: 10.1155/2017/1325754 |
| spellingShingle | GE Environmental Sciences Ngieng, Ngui Sing Ahmad, Husaini Azham, Zulkharnain Hairul Azman, Roslan Decolourisation Capabilities of Ligninolytic Enzymes Produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes |
| title | Decolourisation Capabilities of Ligninolytic Enzymes Produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes |
| title_full | Decolourisation Capabilities of Ligninolytic Enzymes Produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes |
| title_fullStr | Decolourisation Capabilities of Ligninolytic Enzymes Produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes |
| title_full_unstemmed | Decolourisation Capabilities of Ligninolytic Enzymes Produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes |
| title_short | Decolourisation Capabilities of Ligninolytic Enzymes Produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes |
| title_sort | decolourisation capabilities of ligninolytic enzymes produced by marasmius cladophyllus umas ms8 on remazol brilliant blue r and other azo dyes |
| topic | GE Environmental Sciences |
| url | http://ir.unimas.my/id/eprint/15424/ http://ir.unimas.my/id/eprint/15424/ http://ir.unimas.my/id/eprint/15424/ http://ir.unimas.my/id/eprint/15424/7/Decolourisation%20Capabilities%20of%20Ligninolytic%20Enzymes%20Produced%20%28abstract%29.pdf |