Enterovirus A71 and coxsackievirus A16 show different replication kinetics in human neuronal and non-neuronal cell lines
Enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are closely related enteroviruses that cause hand, foot and mouth disease (HFMD) in children. Serious neurological complications almost always occur in EV-A71 infection, but are rare in CV-A16 infection. Based on the hypothesis that this may b...
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| Format: | Article |
| Language: | English |
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Springer-Verlag Wien
2016
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| Online Access: | http://ir.unimas.my/id/eprint/15334/ http://ir.unimas.my/id/eprint/15334/1/kien.pdf |
| _version_ | 1848837833378234368 |
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| author | Yogarajah, Thinesshwary Ong, Kienchai David, Perera WONG, Kumthong |
| author_facet | Yogarajah, Thinesshwary Ong, Kienchai David, Perera WONG, Kumthong |
| author_sort | Yogarajah, Thinesshwary |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are closely related enteroviruses that cause hand, foot and mouth disease (HFMD) in children. Serious neurological complications almost always occur in EV-A71 infection, but are rare in CV-A16 infection. Based on the hypothesis that this may be because EV-A71 infects neuronal cells more easily than CV-A16, we compared virus infection, replication and spread of EV-A71 and CV-A16 in SK-N-SH cells. We found that CV-A16 invariably showed significantly lower replication and caused less necrotic cell death in SK-N-SH cells, compared with EV-A71. This was not due to a lower proportion of CV-A16-infected cells, since both viruses showed similar proportions of infected cells at all time points analyzed. Furthermore, reduced replication of CV-A16 in SK-N-SH cells does not appear to be due to limited viral receptor availability, which might limit viral entry, because experiments with viral RNA-transfected cells showed the same results as for live virus infections. On the other hand, no differences were observed between EV-A71 and CV-A16 in RD cells and results were generally similar in RD cells for both viruses. Taken together, our findings suggest that the poor growth of CV-A16 and EV-A71in SK-N-SH cells, compared with RD cells, may be due to cell type-specific restrictions on viral replication and spread. Furthermore, the lower viral replication and necrotic cell death in CV-A16-infected SK-N-SH cells, compared with EV-A71-infected SK-N-SH cells, is consistent with the lower prevalence of neurotropism observed in CV-A16-associated HFMD outbreaks. Nonetheless, in vivo data and more extensive comparisons of different viral strains are essential to confirm our findings |
| first_indexed | 2025-11-15T06:45:56Z |
| format | Article |
| id | unimas-15334 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:45:56Z |
| publishDate | 2016 |
| publisher | Springer-Verlag Wien |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-153342021-05-21T12:10:09Z http://ir.unimas.my/id/eprint/15334/ Enterovirus A71 and coxsackievirus A16 show different replication kinetics in human neuronal and non-neuronal cell lines Yogarajah, Thinesshwary Ong, Kienchai David, Perera WONG, Kumthong QR180 Immunology Enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are closely related enteroviruses that cause hand, foot and mouth disease (HFMD) in children. Serious neurological complications almost always occur in EV-A71 infection, but are rare in CV-A16 infection. Based on the hypothesis that this may be because EV-A71 infects neuronal cells more easily than CV-A16, we compared virus infection, replication and spread of EV-A71 and CV-A16 in SK-N-SH cells. We found that CV-A16 invariably showed significantly lower replication and caused less necrotic cell death in SK-N-SH cells, compared with EV-A71. This was not due to a lower proportion of CV-A16-infected cells, since both viruses showed similar proportions of infected cells at all time points analyzed. Furthermore, reduced replication of CV-A16 in SK-N-SH cells does not appear to be due to limited viral receptor availability, which might limit viral entry, because experiments with viral RNA-transfected cells showed the same results as for live virus infections. On the other hand, no differences were observed between EV-A71 and CV-A16 in RD cells and results were generally similar in RD cells for both viruses. Taken together, our findings suggest that the poor growth of CV-A16 and EV-A71in SK-N-SH cells, compared with RD cells, may be due to cell type-specific restrictions on viral replication and spread. Furthermore, the lower viral replication and necrotic cell death in CV-A16-infected SK-N-SH cells, compared with EV-A71-infected SK-N-SH cells, is consistent with the lower prevalence of neurotropism observed in CV-A16-associated HFMD outbreaks. Nonetheless, in vivo data and more extensive comparisons of different viral strains are essential to confirm our findings Springer-Verlag Wien 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15334/1/kien.pdf Yogarajah, Thinesshwary and Ong, Kienchai and David, Perera and WONG, Kumthong (2016) Enterovirus A71 and coxsackievirus A16 show different replication kinetics in human neuronal and non-neuronal cell lines. Archives of Virology. pp. 1-11. ISSN 0304-8608 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996590251&doi=10.1007%2fs00705-016-3157-4&partnerID=40&md5=aeac904417893c9795f1a1329a7f14fc DOI: 10.1007/s00705-016-3157-4 |
| spellingShingle | QR180 Immunology Yogarajah, Thinesshwary Ong, Kienchai David, Perera WONG, Kumthong Enterovirus A71 and coxsackievirus A16 show different replication kinetics in human neuronal and non-neuronal cell lines |
| title | Enterovirus A71 and coxsackievirus A16 show different replication kinetics in human neuronal and non-neuronal cell lines |
| title_full | Enterovirus A71 and coxsackievirus A16 show different replication kinetics in human neuronal and non-neuronal cell lines |
| title_fullStr | Enterovirus A71 and coxsackievirus A16 show different replication kinetics in human neuronal and non-neuronal cell lines |
| title_full_unstemmed | Enterovirus A71 and coxsackievirus A16 show different replication kinetics in human neuronal and non-neuronal cell lines |
| title_short | Enterovirus A71 and coxsackievirus A16 show different replication kinetics in human neuronal and non-neuronal cell lines |
| title_sort | enterovirus a71 and coxsackievirus a16 show different replication kinetics in human neuronal and non-neuronal cell lines |
| topic | QR180 Immunology |
| url | http://ir.unimas.my/id/eprint/15334/ http://ir.unimas.my/id/eprint/15334/ http://ir.unimas.my/id/eprint/15334/ http://ir.unimas.my/id/eprint/15334/1/kien.pdf |