Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus
The authors describe a disposable electrochemical immunosensor strip for the detection of the Japanese encephalitis virus (JEV). The assay is based on the use of a screen printed carbon electrode (SPCE) modified with carbon nanoparticles (CNPs) that were prepared from starch nanoparticles and d...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Springer-Verlag
2017
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/15276/ http://ir.unimas.my/id/eprint/15276/7/Carbon%20nanoparticle%20modified%20screen%20printed%20carbon%20electrode%20%28abstract%29.pdf |
| _version_ | 1848837817608699904 |
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| author | Suk, Fun Chin Lih, Shan Lim Suh, Cem Pang Magdline Sia, Henry Sum David, Perera |
| author_facet | Suk, Fun Chin Lih, Shan Lim Suh, Cem Pang Magdline Sia, Henry Sum David, Perera |
| author_sort | Suk, Fun Chin |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The authors describe a disposable electrochemical
immunosensor strip for the detection of the Japanese encephalitis
virus (JEV). The assay is based on the use of a screen
printed carbon electrode (SPCE) modified with carbon nanoparticles
(CNPs) that were prepared from starch nanoparticles
and deposited on the SPCE working electrode whose surface
was functionalized with 3-aminopropyl triethoxysilane. Next,
antibody of JEV was immobilized on the surfaces of the
CNPs. The analytical performance of immunosensor strip
was characterized using cyclic voltammetry (with
hexacyanoferrate as the redox probe) and electrochemical
impedance spectroscopy. The deposition of CNPs
enhances the electron transfer kinetics and current intensity
of the SPCE by 63% compared to an unmodified
SPCE. Under optimized conditions, the calibration plot
is linear within the 5–20 ng·mL−1 JEV concentration range,
the limit of detection being 2 ng·mL−1 (at an S/N ratio of 3),
and the assay time is 20 min. This immunosensor strip was
successfully applied to the detection of JEV in human serum
samples. It represents a cost-effective alternative to conventional
diagnostic tests for JEV. |
| first_indexed | 2025-11-15T06:45:41Z |
| format | Article |
| id | unimas-15276 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:45:41Z |
| publishDate | 2017 |
| publisher | Springer-Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-152762017-08-29T07:24:20Z http://ir.unimas.my/id/eprint/15276/ Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus Suk, Fun Chin Lih, Shan Lim Suh, Cem Pang Magdline Sia, Henry Sum David, Perera Q Science (General) QR180 Immunology QR355 Virology R Medicine (General) The authors describe a disposable electrochemical immunosensor strip for the detection of the Japanese encephalitis virus (JEV). The assay is based on the use of a screen printed carbon electrode (SPCE) modified with carbon nanoparticles (CNPs) that were prepared from starch nanoparticles and deposited on the SPCE working electrode whose surface was functionalized with 3-aminopropyl triethoxysilane. Next, antibody of JEV was immobilized on the surfaces of the CNPs. The analytical performance of immunosensor strip was characterized using cyclic voltammetry (with hexacyanoferrate as the redox probe) and electrochemical impedance spectroscopy. The deposition of CNPs enhances the electron transfer kinetics and current intensity of the SPCE by 63% compared to an unmodified SPCE. Under optimized conditions, the calibration plot is linear within the 5–20 ng·mL−1 JEV concentration range, the limit of detection being 2 ng·mL−1 (at an S/N ratio of 3), and the assay time is 20 min. This immunosensor strip was successfully applied to the detection of JEV in human serum samples. It represents a cost-effective alternative to conventional diagnostic tests for JEV. Springer-Verlag 2017 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15276/7/Carbon%20nanoparticle%20modified%20screen%20printed%20carbon%20electrode%20%28abstract%29.pdf Suk, Fun Chin and Lih, Shan Lim and Suh, Cem Pang and Magdline Sia, Henry Sum and David, Perera (2017) Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus. Microchimica Acta, 184 (2). pp. 491-497. ISSN 1436-5073 https://link.springer.com/article/10.1007/s00604-016-2029-7 DOI 10.1007/s00604-016-2029-7 |
| spellingShingle | Q Science (General) QR180 Immunology QR355 Virology R Medicine (General) Suk, Fun Chin Lih, Shan Lim Suh, Cem Pang Magdline Sia, Henry Sum David, Perera Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus |
| title | Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus |
| title_full | Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus |
| title_fullStr | Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus |
| title_full_unstemmed | Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus |
| title_short | Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus |
| title_sort | carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of japanese encephalitis virus |
| topic | Q Science (General) QR180 Immunology QR355 Virology R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/15276/ http://ir.unimas.my/id/eprint/15276/ http://ir.unimas.my/id/eprint/15276/ http://ir.unimas.my/id/eprint/15276/7/Carbon%20nanoparticle%20modified%20screen%20printed%20carbon%20electrode%20%28abstract%29.pdf |