Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae)
Gas chromatography coupled with mass spectrometry (GC–MS) was used to determine the weathering time in cuticular hydrocarbon of pupae Chrysomya megacephala in sheltered condition. The results have shown that cuticular hydrocarbons (CHC) of the pupae were a mixture of n-alkanes, methyl-branched alk...
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| Format: | Article |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2016
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| Online Access: | http://ir.unimas.my/id/eprint/15031/ http://ir.unimas.my/id/eprint/15031/1/Potential%20Use%20of%20Cuticular%20Hydrocarbons%20in%20Estimating%20the%20Age%20%28abstract%29.pdf |
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| author | Rizoh, bin Bosorang Zaini, Assim Sulaiman, Hanapi Fatimah, Abang |
| author_facet | Rizoh, bin Bosorang Zaini, Assim Sulaiman, Hanapi Fatimah, Abang |
| author_sort | Rizoh, bin Bosorang |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Gas chromatography coupled with mass spectrometry (GC–MS) was used to determine the weathering time in
cuticular hydrocarbon of pupae Chrysomya megacephala in sheltered condition. The results have shown that
cuticular hydrocarbons (CHC) of the pupae were a mixture of n-alkanes, methyl-branched alkanes, and
dimethyl-branched alkanes, with carbon chain length ranging from C19 to C39.The study presents the significant
correlation between the changes pattern in relative abundance of several CHC and development phase in pupae.
Further analysis with multiple linear regression indicated that several CHC compounds showed strong
correlation to blowfly pupae age, which were then utilized to create a prediction equation for the age estimation. Finally, the application of the age-dependent model had revealed that estimated age correlated significantly with
chronological age of samples C. megacephala., y = 0.97x + 0.092, R2 = 0.9698. The study concluded that, CHC
have a potential to estimate age of immature C. megacephala, and possibly in other flies species, and might further be used to determine the PMI. |
| first_indexed | 2025-11-15T06:44:57Z |
| format | Article |
| id | unimas-15031 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:44:57Z |
| publishDate | 2016 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-150312017-02-22T07:19:30Z http://ir.unimas.my/id/eprint/15031/ Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae) Rizoh, bin Bosorang Zaini, Assim Sulaiman, Hanapi Fatimah, Abang QD Chemistry QL Zoology Gas chromatography coupled with mass spectrometry (GC–MS) was used to determine the weathering time in cuticular hydrocarbon of pupae Chrysomya megacephala in sheltered condition. The results have shown that cuticular hydrocarbons (CHC) of the pupae were a mixture of n-alkanes, methyl-branched alkanes, and dimethyl-branched alkanes, with carbon chain length ranging from C19 to C39.The study presents the significant correlation between the changes pattern in relative abundance of several CHC and development phase in pupae. Further analysis with multiple linear regression indicated that several CHC compounds showed strong correlation to blowfly pupae age, which were then utilized to create a prediction equation for the age estimation. Finally, the application of the age-dependent model had revealed that estimated age correlated significantly with chronological age of samples C. megacephala., y = 0.97x + 0.092, R2 = 0.9698. The study concluded that, CHC have a potential to estimate age of immature C. megacephala, and possibly in other flies species, and might further be used to determine the PMI. Universiti Malaysia Sarawak, (UNIMAS) 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/15031/1/Potential%20Use%20of%20Cuticular%20Hydrocarbons%20in%20Estimating%20the%20Age%20%28abstract%29.pdf Rizoh, bin Bosorang and Zaini, Assim and Sulaiman, Hanapi and Fatimah, Abang (2016) Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae). Borneo Journal of Resource Science and Technology, 6 (2). pp. 11-20. ISSN 2229-9769 |
| spellingShingle | QD Chemistry QL Zoology Rizoh, bin Bosorang Zaini, Assim Sulaiman, Hanapi Fatimah, Abang Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae) |
| title | Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae) |
| title_full | Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae) |
| title_fullStr | Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae) |
| title_full_unstemmed | Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae) |
| title_short | Potential Use of Cuticular Hydrocarbons in Estimating the Age of Blowfly Pupae Chrysomya megacephala (Diptera: Calliphoridae) |
| title_sort | potential use of cuticular hydrocarbons in estimating the age of blowfly pupae chrysomya megacephala (diptera: calliphoridae) |
| topic | QD Chemistry QL Zoology |
| url | http://ir.unimas.my/id/eprint/15031/ http://ir.unimas.my/id/eprint/15031/1/Potential%20Use%20of%20Cuticular%20Hydrocarbons%20in%20Estimating%20the%20Age%20%28abstract%29.pdf |