Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: A report of further observations

Background: To investigate the long-term effects on immunity of an inactivated enterovirus 71 (EV71) vaccine and its protective efficacy. Methods: A sub-cohort of 1,100 volunteers from Guangxi Province in China was eligible for enrolment and randomly administered either the EV71 vaccine or a placebo...

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Main Authors: Longding, Liu, Zhaojun, Mo, Zhenglun, Liang, Ying, Zhang, Rongcheng, Li, Kien, Chai Ong, Kum, Thong Wong, Erxia, Yang, Yanchun, Che, Jingjing, Wang, Chenghong, Dong, Min, Feng, Jing, Pu, Lichun, Wang, Yun, Liao, Li, Jiang, Soon, Hao Tan, David, Perera, Teng, Huang, Zhenxin, Zhou, Xuanyi, Wang, Jielai, Xia, Lei, Guo, Ling, Wang, Zhongping, Xie, Wei, Cui, Qunying, Mao, Yan, Liang, Hongling, Zhao, Ruixiong, Na, Pingfang, Cui, Haijing, Shi, Junzhi, Wang, Qihan, Li
Format: Article
Language:English
Published: BioMed Central Ltd. 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/14915/
http://ir.unimas.my/id/eprint/14915/1/Immunity.pdf
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author Longding, Liu
Zhaojun, Mo
Zhenglun, Liang
Ying, Zhang
Rongcheng, Li
Kien, Chai Ong
Kum, Thong Wong
Erxia, Yang
Yanchun, Che
Jingjing, Wang
Chenghong, Dong
Min, Feng
Jing, Pu
Lichun, Wang
Yun, Liao
Li, Jiang
Soon, Hao Tan
David, Perera
Teng, Huang
Zhenxin, Zhou
Xuanyi, Wang
Jielai, Xia
Lei, Guo
Ling, Wang
Zhongping, Xie
Wei, Cui
Qunying, Mao
Yan, Liang
Hongling, Zhao
Ruixiong, Na
Pingfang, Cui
Haijing, Shi
Junzhi, Wang
Qihan, Li
author_facet Longding, Liu
Zhaojun, Mo
Zhenglun, Liang
Ying, Zhang
Rongcheng, Li
Kien, Chai Ong
Kum, Thong Wong
Erxia, Yang
Yanchun, Che
Jingjing, Wang
Chenghong, Dong
Min, Feng
Jing, Pu
Lichun, Wang
Yun, Liao
Li, Jiang
Soon, Hao Tan
David, Perera
Teng, Huang
Zhenxin, Zhou
Xuanyi, Wang
Jielai, Xia
Lei, Guo
Ling, Wang
Zhongping, Xie
Wei, Cui
Qunying, Mao
Yan, Liang
Hongling, Zhao
Ruixiong, Na
Pingfang, Cui
Haijing, Shi
Junzhi, Wang
Qihan, Li
author_sort Longding, Liu
building UNIMAS Institutional Repository
collection Online Access
description Background: To investigate the long-term effects on immunity of an inactivated enterovirus 71 (EV71) vaccine and its protective efficacy. Methods: A sub-cohort of 1,100 volunteers from Guangxi Province in China was eligible for enrolment and randomly administered either the EV71 vaccine or a placebo on days 0 and 28 in a phase III clinical trial and then observed for the following 2 years with approval by an independent ethics committee of Guangxi Zhuang Autonomous Region, China. Serum samples from the 350 participants who provided a full series of blood samples (at all the sampling points) within the 2-year period were collected. Vaccine-induced immune effects, including the neutralizing antibody titres and cross-protection against different genotypes of EV71, were examined. This study also evaluated the protective efficacy of this vaccine based upon clinical diagnosis. Results: This sub-cohort showed a >60 % drop-out rate over 2 years. The seroconversion rates among the 161 immunized subjects remained >95 % at the end of study. The geometric mean titres of neutralizing antibodies (anti-genotype C4) 360 days after vaccination in 350 subjects were 81.0 (subjects aged 6-11 months), 98.4 (12-23 months), 95.0 (24-35 months), and 81.8 (36-71 months). These titres subsequently increased to 423.1, 659.0, 545.0, and 321.9, respectively, at 540 days post-immunization (d.p.i.), and similar levels were maintained at 720 d.p.i. Higher IFN-γ/IL-4-specific responses to the C4 genotype of EV71 and cross-neutralization reactivity against major EV71 genotype strains were observed in the vaccine group compared to those in the placebo group. Five EV71-infected subjects were observed in the placebo-treated control group and none in the vaccine-immunized group in per-protocol analysis. Conclusion: These results are consistent with the induction of dynamic immune responses and protective efficacy of the vaccine against most circulating EV71 strains. Trial registration number: Clinicaltrials.gov, NCT01569581 , Trial registration date: March 2012 © 2015 Liu et al.
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publisher BioMed Central Ltd.
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spelling unimas-149152022-01-28T01:37:46Z http://ir.unimas.my/id/eprint/14915/ Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: A report of further observations Longding, Liu Zhaojun, Mo Zhenglun, Liang Ying, Zhang Rongcheng, Li Kien, Chai Ong Kum, Thong Wong Erxia, Yang Yanchun, Che Jingjing, Wang Chenghong, Dong Min, Feng Jing, Pu Lichun, Wang Yun, Liao Li, Jiang Soon, Hao Tan David, Perera Teng, Huang Zhenxin, Zhou Xuanyi, Wang Jielai, Xia Lei, Guo Ling, Wang Zhongping, Xie Wei, Cui Qunying, Mao Yan, Liang Hongling, Zhao Ruixiong, Na Pingfang, Cui Haijing, Shi Junzhi, Wang Qihan, Li RB Pathology RM Therapeutics. Pharmacology Background: To investigate the long-term effects on immunity of an inactivated enterovirus 71 (EV71) vaccine and its protective efficacy. Methods: A sub-cohort of 1,100 volunteers from Guangxi Province in China was eligible for enrolment and randomly administered either the EV71 vaccine or a placebo on days 0 and 28 in a phase III clinical trial and then observed for the following 2 years with approval by an independent ethics committee of Guangxi Zhuang Autonomous Region, China. Serum samples from the 350 participants who provided a full series of blood samples (at all the sampling points) within the 2-year period were collected. Vaccine-induced immune effects, including the neutralizing antibody titres and cross-protection against different genotypes of EV71, were examined. This study also evaluated the protective efficacy of this vaccine based upon clinical diagnosis. Results: This sub-cohort showed a >60 % drop-out rate over 2 years. The seroconversion rates among the 161 immunized subjects remained >95 % at the end of study. The geometric mean titres of neutralizing antibodies (anti-genotype C4) 360 days after vaccination in 350 subjects were 81.0 (subjects aged 6-11 months), 98.4 (12-23 months), 95.0 (24-35 months), and 81.8 (36-71 months). These titres subsequently increased to 423.1, 659.0, 545.0, and 321.9, respectively, at 540 days post-immunization (d.p.i.), and similar levels were maintained at 720 d.p.i. Higher IFN-γ/IL-4-specific responses to the C4 genotype of EV71 and cross-neutralization reactivity against major EV71 genotype strains were observed in the vaccine group compared to those in the placebo group. Five EV71-infected subjects were observed in the placebo-treated control group and none in the vaccine-immunized group in per-protocol analysis. Conclusion: These results are consistent with the induction of dynamic immune responses and protective efficacy of the vaccine against most circulating EV71 strains. Trial registration number: Clinicaltrials.gov, NCT01569581 , Trial registration date: March 2012 © 2015 Liu et al. BioMed Central Ltd. 2015 Article PeerReviewed text en http://ir.unimas.my/id/eprint/14915/1/Immunity.pdf Longding, Liu and Zhaojun, Mo and Zhenglun, Liang and Ying, Zhang and Rongcheng, Li and Kien, Chai Ong and Kum, Thong Wong and Erxia, Yang and Yanchun, Che and Jingjing, Wang and Chenghong, Dong and Min, Feng and Jing, Pu and Lichun, Wang and Yun, Liao and Li, Jiang and Soon, Hao Tan and David, Perera and Teng, Huang and Zhenxin, Zhou and Xuanyi, Wang and Jielai, Xia and Lei, Guo and Ling, Wang and Zhongping, Xie and Wei, Cui and Qunying, Mao and Yan, Liang and Hongling, Zhao and Ruixiong, Na and Pingfang, Cui and Haijing, Shi and Junzhi, Wang and Qihan, Li (2015) Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: A report of further observations. BMC Medicine, 13 (1). pp. 1-10. ISSN 17417015 http://www.scopus.com/inward/record.url?eid=2-s2.0-84942029186&partnerID=40&md5=d6e7ffb287c3b148ae023f4e96cdcd7c
spellingShingle RB Pathology
RM Therapeutics. Pharmacology
Longding, Liu
Zhaojun, Mo
Zhenglun, Liang
Ying, Zhang
Rongcheng, Li
Kien, Chai Ong
Kum, Thong Wong
Erxia, Yang
Yanchun, Che
Jingjing, Wang
Chenghong, Dong
Min, Feng
Jing, Pu
Lichun, Wang
Yun, Liao
Li, Jiang
Soon, Hao Tan
David, Perera
Teng, Huang
Zhenxin, Zhou
Xuanyi, Wang
Jielai, Xia
Lei, Guo
Ling, Wang
Zhongping, Xie
Wei, Cui
Qunying, Mao
Yan, Liang
Hongling, Zhao
Ruixiong, Na
Pingfang, Cui
Haijing, Shi
Junzhi, Wang
Qihan, Li
Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: A report of further observations
title Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: A report of further observations
title_full Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: A report of further observations
title_fullStr Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: A report of further observations
title_full_unstemmed Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: A report of further observations
title_short Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: A report of further observations
title_sort immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy chinese children: a report of further observations
topic RB Pathology
RM Therapeutics. Pharmacology
url http://ir.unimas.my/id/eprint/14915/
http://ir.unimas.my/id/eprint/14915/
http://ir.unimas.my/id/eprint/14915/1/Immunity.pdf