Micropropagation of red ginger (zingiber montanum koenig), a medicinal plant
A protocol has been developed for the in vitro regeneration of red ginger (Zingiber montanum Koenig), a valuable medicinal plant. Rhizome buds of 1.5 to 2 cm long were surface sterilized with commercial bleach prior to culture on Gamborg B5 medium incorporated with Tetracycline at 15 ml/L and Plan...
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| Format: | Article |
| Language: | English |
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APJMBB
2010
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| Online Access: | http://ir.unimas.my/id/eprint/14387/ http://ir.unimas.my/id/eprint/14387/1/Micropropagation%20of%20red%20ginger%20%28abstract%29.pdf |
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| author | Hamirah, M.N. Hamsawi, Sani Boyce, P.C Sim, S.L. |
| author_facet | Hamirah, M.N. Hamsawi, Sani Boyce, P.C Sim, S.L. |
| author_sort | Hamirah, M.N. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A protocol has been developed for the in vitro regeneration of red ginger (Zingiber montanum Koenig), a valuable
medicinal plant. Rhizome buds of 1.5 to 2 cm long were surface sterilized with commercial bleach prior to culture on Gamborg
B5 medium incorporated with Tetracycline at 15 ml/L and Plant Preservative Mixture at 2 ml/L. For induction of multiple
shoots, three studies were conducted, (1) effects of different plant growth regulators, (2) effect sof whole and sectioned buds and
(3) effects of culture phase. Thidiazuron (TDZ) at 0.5 mg/L was found to induce the highest shoot multiplication with a mean of
8.1 shoots per explant. Sectioned buds produced a mean of 4.6 shoots from each explant. As far the culture phase, liquid medium
was found to be superior to solid medium. Rooting of propagules was conducted on B5 medium devoid of growth regulators.
Acclimatization was conducted on medium containing a mixture of 1:1:1 soil, sand and peat with about 85% survivability. |
| first_indexed | 2025-11-15T06:42:49Z |
| format | Article |
| id | unimas-14387 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:42:49Z |
| publishDate | 2010 |
| publisher | APJMBB |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-143872016-11-22T03:46:18Z http://ir.unimas.my/id/eprint/14387/ Micropropagation of red ginger (zingiber montanum koenig), a medicinal plant Hamirah, M.N. Hamsawi, Sani Boyce, P.C Sim, S.L. SB Plant culture A protocol has been developed for the in vitro regeneration of red ginger (Zingiber montanum Koenig), a valuable medicinal plant. Rhizome buds of 1.5 to 2 cm long were surface sterilized with commercial bleach prior to culture on Gamborg B5 medium incorporated with Tetracycline at 15 ml/L and Plant Preservative Mixture at 2 ml/L. For induction of multiple shoots, three studies were conducted, (1) effects of different plant growth regulators, (2) effect sof whole and sectioned buds and (3) effects of culture phase. Thidiazuron (TDZ) at 0.5 mg/L was found to induce the highest shoot multiplication with a mean of 8.1 shoots per explant. Sectioned buds produced a mean of 4.6 shoots from each explant. As far the culture phase, liquid medium was found to be superior to solid medium. Rooting of propagules was conducted on B5 medium devoid of growth regulators. Acclimatization was conducted on medium containing a mixture of 1:1:1 soil, sand and peat with about 85% survivability. APJMBB 2010 Article PeerReviewed text en http://ir.unimas.my/id/eprint/14387/1/Micropropagation%20of%20red%20ginger%20%28abstract%29.pdf Hamirah, M.N. and Hamsawi, Sani and Boyce, P.C and Sim, S.L. (2010) Micropropagation of red ginger (zingiber montanum koenig), a medicinal plant. Asia Pacific Molecular Biology and Biotechnology, 18 (1). pp. 127-130. ISSN 0128-7451 https://www.researchgate.net/publication/288752570_Micropropagation_of_red_ginger_zingiber_montanum_koenig_a_medicinal_plant |
| spellingShingle | SB Plant culture Hamirah, M.N. Hamsawi, Sani Boyce, P.C Sim, S.L. Micropropagation of red ginger (zingiber montanum koenig), a medicinal plant |
| title | Micropropagation of red ginger (zingiber montanum koenig), a medicinal plant |
| title_full | Micropropagation of red ginger (zingiber montanum koenig), a medicinal plant |
| title_fullStr | Micropropagation of red ginger (zingiber montanum koenig), a medicinal plant |
| title_full_unstemmed | Micropropagation of red ginger (zingiber montanum koenig), a medicinal plant |
| title_short | Micropropagation of red ginger (zingiber montanum koenig), a medicinal plant |
| title_sort | micropropagation of red ginger (zingiber montanum koenig), a medicinal plant |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/14387/ http://ir.unimas.my/id/eprint/14387/ http://ir.unimas.my/id/eprint/14387/1/Micropropagation%20of%20red%20ginger%20%28abstract%29.pdf |