An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer
Isolation of high-quality genomic DNA from Dryobalanops beccarii is obviously difficult due to the existence of large amounts of camphor and other secondary metabolites. These contaminants will co-precipitate with DNA during DNA isolation and purification processes, and therefore, resulting in a bro...
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| Format: | Article |
| Language: | English |
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IJSTR
2016
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| Online Access: | http://ir.unimas.my/id/eprint/14331/ http://ir.unimas.my/id/eprint/14331/1/An%20Optimized%20DNA%20Extraction%20Protocol%20For%20Isolation%20Of%20High%20Quality%20%28abstract%29.pdf |
| _version_ | 1848837624416960512 |
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| author | Wei-Seng, Ho Kit-Siong, Liew Shek-Ling, Pang |
| author_facet | Wei-Seng, Ho Kit-Siong, Liew Shek-Ling, Pang |
| author_sort | Wei-Seng, Ho |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Isolation of high-quality genomic DNA from Dryobalanops beccarii is obviously difficult due to the existence of large amounts of camphor and other secondary metabolites. These contaminants will co-precipitate with DNA during DNA isolation and purification processes, and therefore, resulting in a brownish DNA pellet that is unsuitable for downstream applications. Many DNA isolation protocols are available for various plant tissues; however these protocols are inefficient in yielding high-quality amplifiable genomic DNA especially from camphor containing timber tree species. A CTAB based protocol has been optimized for isolating genomic DNA from camphor containing timber tree species. Key steps include: 1) using 1% β-mercaptoethanol and 2% PVP 40 (Mr 40,000) in the extraction buffer; 2) sample incubation time, 40 minutes at 65°C, and 3) DNA precipitation at room temperature (25°C). The isolated DNA pellet was transparent colour and the purified genomic DNA is suitable for PCR amplification.
Wei-Seng Ho, Kit-Siong Liew, Shek-Ling Pang |
| first_indexed | 2025-11-15T06:42:37Z |
| format | Article |
| id | unimas-14331 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:42:37Z |
| publishDate | 2016 |
| publisher | IJSTR |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-143312016-11-18T01:26:14Z http://ir.unimas.my/id/eprint/14331/ An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer Wei-Seng, Ho Kit-Siong, Liew Shek-Ling, Pang GE Environmental Sciences Q Science (General) Isolation of high-quality genomic DNA from Dryobalanops beccarii is obviously difficult due to the existence of large amounts of camphor and other secondary metabolites. These contaminants will co-precipitate with DNA during DNA isolation and purification processes, and therefore, resulting in a brownish DNA pellet that is unsuitable for downstream applications. Many DNA isolation protocols are available for various plant tissues; however these protocols are inefficient in yielding high-quality amplifiable genomic DNA especially from camphor containing timber tree species. A CTAB based protocol has been optimized for isolating genomic DNA from camphor containing timber tree species. Key steps include: 1) using 1% β-mercaptoethanol and 2% PVP 40 (Mr 40,000) in the extraction buffer; 2) sample incubation time, 40 minutes at 65°C, and 3) DNA precipitation at room temperature (25°C). The isolated DNA pellet was transparent colour and the purified genomic DNA is suitable for PCR amplification. Wei-Seng Ho, Kit-Siong Liew, Shek-Ling Pang IJSTR 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/14331/1/An%20Optimized%20DNA%20Extraction%20Protocol%20For%20Isolation%20Of%20High%20Quality%20%28abstract%29.pdf Wei-Seng, Ho and Kit-Siong, Liew and Shek-Ling, Pang (2016) An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer. International Journal Of Scientific & Technology Research, 5 (9). pp. 220-223. ISSN 2277-8616 http://www.ijstr.org/research-paper-publishing.php?month=sep2016 |
| spellingShingle | GE Environmental Sciences Q Science (General) Wei-Seng, Ho Kit-Siong, Liew Shek-Ling, Pang An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer |
| title | An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer |
| title_full | An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer |
| title_fullStr | An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer |
| title_full_unstemmed | An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer |
| title_short | An Optimized DNA Extraction Protocol For Isolation Of High Quality Genomic DNA From Camphor Containing Timber Tree Species, Dryobalanops Beccarii Dyer |
| title_sort | optimized dna extraction protocol for isolation of high quality genomic dna from camphor containing timber tree species, dryobalanops beccarii dyer |
| topic | GE Environmental Sciences Q Science (General) |
| url | http://ir.unimas.my/id/eprint/14331/ http://ir.unimas.my/id/eprint/14331/ http://ir.unimas.my/id/eprint/14331/1/An%20Optimized%20DNA%20Extraction%20Protocol%20For%20Isolation%20Of%20High%20Quality%20%28abstract%29.pdf |