Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis
The combined overlap extension PCR (COE-PCR) method developed in this work combines the strengths of the overlap extension PCR (OE-PCR) method with the speed and ease of the asymmetrical overlap extension (AOE-PCR) method. This combined method allows up to 6 base pairs to be mutated at a time and re...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://ir.unimas.my/id/eprint/14294/ http://ir.unimas.my/id/eprint/14294/1/Combined%20Overlap%20Extension%20PCR%20Method%20for%20Improved%20Site%20Directed%20Mutagenesis%20%28abstract%29.pdf |
| _version_ | 1848837614846607360 |
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| author | Hasnain, Hussain Chong, Nikson Fatt Ming |
| author_facet | Hasnain, Hussain Chong, Nikson Fatt Ming |
| author_sort | Hasnain, Hussain |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The combined overlap extension PCR (COE-PCR) method developed in this work combines the strengths of the overlap extension PCR (OE-PCR) method with the speed and ease of the asymmetrical overlap extension (AOE-PCR) method. This combined method allows up to 6 base pairs to be mutated at a time and requires a total of 40- 45 PCR cycles. A total of eight mutagenesis experiments were successfully carried out, with each experiment mutating between two to six base pairs. Up to four adjacent codons were changed in a single experiment. This method is especially useful for codon optimization, where doublet or triplet rare codons can be changed using a single mutagenic primer-set, in a single experiment. |
| first_indexed | 2025-11-15T06:42:28Z |
| format | Article |
| id | unimas-14294 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:42:28Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-142942017-02-17T02:43:35Z http://ir.unimas.my/id/eprint/14294/ Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis Hasnain, Hussain Chong, Nikson Fatt Ming Q Science (General) QR Microbiology The combined overlap extension PCR (COE-PCR) method developed in this work combines the strengths of the overlap extension PCR (OE-PCR) method with the speed and ease of the asymmetrical overlap extension (AOE-PCR) method. This combined method allows up to 6 base pairs to be mutated at a time and requires a total of 40- 45 PCR cycles. A total of eight mutagenesis experiments were successfully carried out, with each experiment mutating between two to six base pairs. Up to four adjacent codons were changed in a single experiment. This method is especially useful for codon optimization, where doublet or triplet rare codons can be changed using a single mutagenic primer-set, in a single experiment. Hindawi Publishing Corporation 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/14294/1/Combined%20Overlap%20Extension%20PCR%20Method%20for%20Improved%20Site%20Directed%20Mutagenesis%20%28abstract%29.pdf Hasnain, Hussain and Chong, Nikson Fatt Ming (2016) Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis. BioMed Research International, 2016. ISSN 2314-6133 https://www.hindawi.com/journals/bmri/ |
| spellingShingle | Q Science (General) QR Microbiology Hasnain, Hussain Chong, Nikson Fatt Ming Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis |
| title | Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis |
| title_full | Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis |
| title_fullStr | Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis |
| title_full_unstemmed | Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis |
| title_short | Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis |
| title_sort | combined overlap extension pcr method for improved site directed mutagenesis |
| topic | Q Science (General) QR Microbiology |
| url | http://ir.unimas.my/id/eprint/14294/ http://ir.unimas.my/id/eprint/14294/ http://ir.unimas.my/id/eprint/14294/1/Combined%20Overlap%20Extension%20PCR%20Method%20for%20Improved%20Site%20Directed%20Mutagenesis%20%28abstract%29.pdf |