Isolation of xet gene involved in wood formation in shorea parvifolia dyer pervioflia
Wood formation genes played an important internal factor besides the hormonal aspects in xylogenesis, which control the development of secondary growth in trees. One of the identified enzymes, xyloglucan endotransglycosylase (XET), is discovered to modify intermicrofibrillar xyloglucan chains, a...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2005
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/1428/ http://ir.unimas.my/id/eprint/1428/8/2013-03-prChiaSWfull.pdf |
| _version_ | 1848834758387171328 |
|---|---|
| author | Chia, Sze Wooi |
| author_facet | Chia, Sze Wooi |
| author_sort | Chia, Sze Wooi |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Wood formation genes played an important internal factor besides the hormonal
aspects in xylogenesis, which control the development of secondary growth in trees.
One of the identified enzymes, xyloglucan endotransglycosylase (XET), is discovered
to modify intermicrofibrillar xyloglucan chains, a major component of primary cell
walls in dicots to allow wall-loosening required for plant cell expansion. In this study,
Shorea parvifblia Dyer parvifolia obtained from Sarawak Forest Seed Bank was
chosen due to its economical value and strong adaptability. The total genomic DNA
was extracted using a modified CTAB method. A pair of primers, i. e. forward primer
(5' - TGGTGACTCAGCTGGAACAG - 3') and reverse primer (5' -
AATCATCGGCATTCCATAGG - 3') was constructed based on the known XFT
mRNA sequences obtained from the databases. Polymerase Chain Reaction (PCR)
was performed based on the optimized thermo-cycling profile as follow: 35 cycles of
I min of denaturing at 94°C, 1 min of annealing at 46°C, and 2 min extension phase at
72°C. A DNA fragment of - -527bp was obtained from the amplification and was
cloned using a TA-vector system. Then, the isolated and purified plasmid was sent for
sequencing. |
| first_indexed | 2025-11-15T05:57:04Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-1428 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T05:57:04Z |
| publishDate | 2005 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-14282023-02-13T04:16:06Z http://ir.unimas.my/id/eprint/1428/ Isolation of xet gene involved in wood formation in shorea parvifolia dyer pervioflia Chia, Sze Wooi GE Environmental Sciences Wood formation genes played an important internal factor besides the hormonal aspects in xylogenesis, which control the development of secondary growth in trees. One of the identified enzymes, xyloglucan endotransglycosylase (XET), is discovered to modify intermicrofibrillar xyloglucan chains, a major component of primary cell walls in dicots to allow wall-loosening required for plant cell expansion. In this study, Shorea parvifblia Dyer parvifolia obtained from Sarawak Forest Seed Bank was chosen due to its economical value and strong adaptability. The total genomic DNA was extracted using a modified CTAB method. A pair of primers, i. e. forward primer (5' - TGGTGACTCAGCTGGAACAG - 3') and reverse primer (5' - AATCATCGGCATTCCATAGG - 3') was constructed based on the known XFT mRNA sequences obtained from the databases. Polymerase Chain Reaction (PCR) was performed based on the optimized thermo-cycling profile as follow: 35 cycles of I min of denaturing at 94°C, 1 min of annealing at 46°C, and 2 min extension phase at 72°C. A DNA fragment of - -527bp was obtained from the amplification and was cloned using a TA-vector system. Then, the isolated and purified plasmid was sent for sequencing. Universiti Malaysia Sarawak, (UNIMAS) 2005 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/1428/8/2013-03-prChiaSWfull.pdf Chia, Sze Wooi (2005) Isolation of xet gene involved in wood formation in shorea parvifolia dyer pervioflia. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences Chia, Sze Wooi Isolation of xet gene involved in wood formation in shorea parvifolia dyer pervioflia |
| title | Isolation of xet gene involved in wood formation in shorea parvifolia dyer pervioflia |
| title_full | Isolation of xet gene involved in wood formation in shorea parvifolia dyer pervioflia |
| title_fullStr | Isolation of xet gene involved in wood formation in shorea parvifolia dyer pervioflia |
| title_full_unstemmed | Isolation of xet gene involved in wood formation in shorea parvifolia dyer pervioflia |
| title_short | Isolation of xet gene involved in wood formation in shorea parvifolia dyer pervioflia |
| title_sort | isolation of xet gene involved in wood formation in shorea parvifolia dyer pervioflia |
| topic | GE Environmental Sciences |
| url | http://ir.unimas.my/id/eprint/1428/ http://ir.unimas.my/id/eprint/1428/8/2013-03-prChiaSWfull.pdf |