Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides
Twenty two bacterial strains isolated from peat soils in a pineapple plantation were analyzed. Fourteen of the Gram-negative strains were tentatively classified into 12 genera that included Thiobacillus, Acinetobacter, Acidiphilium, Enterobacter, and Pseudomonas. 91% of the isolates were able to...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2004
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/1420/ http://ir.unimas.my/id/eprint/1420/8/2013-03-ChiaSYfull.pdf |
| _version_ | 1848834756965302272 |
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| author | Chia, Soo Yee |
| author_facet | Chia, Soo Yee |
| author_sort | Chia, Soo Yee |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Twenty two bacterial strains isolated from peat soils in a pineapple plantation were analyzed. Fourteen
of the Gram-negative strains were tentatively classified into 12 genera that included Thiobacillus,
Acinetobacter, Acidiphilium, Enterobacter, and Pseudomonas. 91% of the isolates were able to
tolerate 2,4-dimethylamine with concentrations of up to 10,000 ppm, while 60% of the isolates were
resistant to malathion and chlorpyrifos with concentrations of up to 50,000 ppm. As for antibiotics,
82% of the isolates able to survive in carbenicillin agar with concentrations of 1000 ug/ml. 70% of the
isolates can survive in both tetracycline and streptomycin media. Four bacterial isolates, C105
(Acidiphilium cryptum), C102 (Sulpholobus spp), C108 (Leptospirillum spp) and Y104 (Pseudomonas
flouresc/putida) that were pesticide resistant were analyzed for the presence of plasmid. No plasmid
was detected in antibiotic resistant isolates except for strains designated as Y 103 and C 105. The sizes
of the isolated plasmids were estimated ranging from 6 kb to 54 kb. Further studies are required to
investigate the genes which confer resistance properties in the acidophiles. |
| first_indexed | 2025-11-15T05:57:02Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-1420 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T05:57:02Z |
| publishDate | 2004 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-14202023-02-14T07:43:26Z http://ir.unimas.my/id/eprint/1420/ Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides Chia, Soo Yee Q Science (General) Twenty two bacterial strains isolated from peat soils in a pineapple plantation were analyzed. Fourteen of the Gram-negative strains were tentatively classified into 12 genera that included Thiobacillus, Acinetobacter, Acidiphilium, Enterobacter, and Pseudomonas. 91% of the isolates were able to tolerate 2,4-dimethylamine with concentrations of up to 10,000 ppm, while 60% of the isolates were resistant to malathion and chlorpyrifos with concentrations of up to 50,000 ppm. As for antibiotics, 82% of the isolates able to survive in carbenicillin agar with concentrations of 1000 ug/ml. 70% of the isolates can survive in both tetracycline and streptomycin media. Four bacterial isolates, C105 (Acidiphilium cryptum), C102 (Sulpholobus spp), C108 (Leptospirillum spp) and Y104 (Pseudomonas flouresc/putida) that were pesticide resistant were analyzed for the presence of plasmid. No plasmid was detected in antibiotic resistant isolates except for strains designated as Y 103 and C 105. The sizes of the isolated plasmids were estimated ranging from 6 kb to 54 kb. Further studies are required to investigate the genes which confer resistance properties in the acidophiles. Universiti Malaysia Sarawak, (UNIMAS) 2004 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/1420/8/2013-03-ChiaSYfull.pdf Chia, Soo Yee (2004) Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) Chia, Soo Yee Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides |
| title | Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides |
| title_full | Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides |
| title_fullStr | Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides |
| title_full_unstemmed | Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides |
| title_short | Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides |
| title_sort | isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/1420/ http://ir.unimas.my/id/eprint/1420/8/2013-03-ChiaSYfull.pdf |