Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu
Alcoholic fermentattion in plants with reduction of acetaldehyde by alcohol dehydrogenase to produce energy is one of the metabolic pathways that enables plant to withstand stress. Sago plam is important to the state of Sarawak as one of the most important crops that bring huge revenue. It has the a...
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| Format: | Thesis |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2011
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| Online Access: | http://ir.unimas.my/id/eprint/14139/ http://ir.unimas.my/id/eprint/14139/4/Ching%20Ching%20%28fulltext%29.pdf |
| _version_ | 1848837577554001920 |
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| author | Wee, Ching Ching. |
| author_facet | Wee, Ching Ching. |
| author_sort | Wee, Ching Ching. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Alcoholic fermentattion in plants with reduction of acetaldehyde by alcohol dehydrogenase to produce energy is one of the metabolic pathways that enables plant to withstand stress. Sago plam is important to the state of Sarawak as one of the most important crops that bring huge revenue. It has the ability to withstand stress by growing well in waterlogged area. Shoots of sago palm in the early development stages has a high alcohol dehydrogenase gene expression. In this study, the gene expression in selected sago palm tissue was studied via the generation of expressed sequence tags of young leaves and isolation of full length adh cDNA from sago palm leaves using a combination of RACE and DNA walking method. |
| first_indexed | 2025-11-15T06:41:52Z |
| format | Thesis |
| id | unimas-14139 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:41:52Z |
| publishDate | 2011 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-141392023-05-18T09:24:05Z http://ir.unimas.my/id/eprint/14139/ Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu Wee, Ching Ching. SB Plant culture Alcoholic fermentattion in plants with reduction of acetaldehyde by alcohol dehydrogenase to produce energy is one of the metabolic pathways that enables plant to withstand stress. Sago plam is important to the state of Sarawak as one of the most important crops that bring huge revenue. It has the ability to withstand stress by growing well in waterlogged area. Shoots of sago palm in the early development stages has a high alcohol dehydrogenase gene expression. In this study, the gene expression in selected sago palm tissue was studied via the generation of expressed sequence tags of young leaves and isolation of full length adh cDNA from sago palm leaves using a combination of RACE and DNA walking method. Universiti Malaysia Sarawak, (UNIMAS) 2011 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/14139/4/Ching%20Ching%20%28fulltext%29.pdf Wee, Ching Ching. (2011) Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu. Masters thesis, Universiti Malaysia Sarawak, (UNIMAS). |
| spellingShingle | SB Plant culture Wee, Ching Ching. Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu |
| title | Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu |
| title_full | Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu |
| title_fullStr | Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu |
| title_full_unstemmed | Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu |
| title_short | Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu |
| title_sort | generation of expressed sequence tags (ests) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/14139/ http://ir.unimas.my/id/eprint/14139/4/Ching%20Ching%20%28fulltext%29.pdf |