Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR
Background: Human enterovirus 71 has emerged as an important pathogen in the Asia Pacific region and it is important to be able to make a rapid and specific diagnosis for outbreak control. Recent Asian strains of Coxsackievirus A16 have changes in the VP1 gene which causes mispriming of widely us...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BioMed Central Ltd.
2004
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| Online Access: | http://ir.unimas.my/id/eprint/13620/ http://ir.unimas.my/id/eprint/13620/1/Incorrect%20identification%20of%20recent%20Asian%20strains%20of%20Coxsackievirus%20%28abstract%29.pdf |
| _version_ | 1848837445040209920 |
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| author | David, Perera Yuwana, Podin Winnie, Akin Tan, Cheng-Siang Cardosa, Mary Jane |
| author_facet | David, Perera Yuwana, Podin Winnie, Akin Tan, Cheng-Siang Cardosa, Mary Jane |
| author_sort | David, Perera |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Background: Human enterovirus 71 has emerged as an important pathogen in the Asia Pacific
region and it is important to be able to make a rapid and specific diagnosis for outbreak control.
Recent Asian strains of Coxsackievirus A16 have changes in the VP1 gene which causes mispriming
of widely used primers for human enterovirus 71 specific identification.
Methods: Local strains of Coxsackievirus A16 were sequenced in the VP4 and VP1 genes and using
sequence alignment tools, an improved set of primers were designed for specific identification of
human enterovirus 71. These primers were evaluated against virus isolates as well as primary
clinical specimens.
Results: A total of 218 virus strains were tested. All 39 human enterovirus 71 isolates were
positive and none of the 38 Coxsackievirus A16, 127 other enteroviruses and 14 prototype
flaviviruses and adenoviruses were positive when tested with the new primers. When aliquots of
primary specimens known to have yielded human enterovirus 71 were retrospectively tested, we
found that within 2 months of collection of the specimens, greater than 90% were positive but that
the success rate diminished rapidly to 18% after 2 years storage.
Conclusions: Our new primers will be useful in rapid diagnosis of human enterovirus 71 infection,
and can also be used as a screening tool in surveillance programmes for early warning of human
enterovirus 71 transmission. |
| first_indexed | 2025-11-15T06:39:46Z |
| format | Article |
| id | unimas-13620 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:39:46Z |
| publishDate | 2004 |
| publisher | BioMed Central Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-136202016-09-26T22:18:45Z http://ir.unimas.my/id/eprint/13620/ Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR David, Perera Yuwana, Podin Winnie, Akin Tan, Cheng-Siang Cardosa, Mary Jane RJ101 Child Health. Child health services Background: Human enterovirus 71 has emerged as an important pathogen in the Asia Pacific region and it is important to be able to make a rapid and specific diagnosis for outbreak control. Recent Asian strains of Coxsackievirus A16 have changes in the VP1 gene which causes mispriming of widely used primers for human enterovirus 71 specific identification. Methods: Local strains of Coxsackievirus A16 were sequenced in the VP4 and VP1 genes and using sequence alignment tools, an improved set of primers were designed for specific identification of human enterovirus 71. These primers were evaluated against virus isolates as well as primary clinical specimens. Results: A total of 218 virus strains were tested. All 39 human enterovirus 71 isolates were positive and none of the 38 Coxsackievirus A16, 127 other enteroviruses and 14 prototype flaviviruses and adenoviruses were positive when tested with the new primers. When aliquots of primary specimens known to have yielded human enterovirus 71 were retrospectively tested, we found that within 2 months of collection of the specimens, greater than 90% were positive but that the success rate diminished rapidly to 18% after 2 years storage. Conclusions: Our new primers will be useful in rapid diagnosis of human enterovirus 71 infection, and can also be used as a screening tool in surveillance programmes for early warning of human enterovirus 71 transmission. BioMed Central Ltd. 2004 Article PeerReviewed text en http://ir.unimas.my/id/eprint/13620/1/Incorrect%20identification%20of%20recent%20Asian%20strains%20of%20Coxsackievirus%20%28abstract%29.pdf David, Perera and Yuwana, Podin and Winnie, Akin and Tan, Cheng-Siang and Cardosa, Mary Jane (2004) Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR. BMC Infectious Diseases, 4. pp. 1-11. ISSN 13640321 http://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-4-11 DOI: 10.1186/1471-2334-4-11 |
| spellingShingle | RJ101 Child Health. Child health services David, Perera Yuwana, Podin Winnie, Akin Tan, Cheng-Siang Cardosa, Mary Jane Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR |
| title | Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR |
| title_full | Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR |
| title_fullStr | Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR |
| title_full_unstemmed | Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR |
| title_short | Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR |
| title_sort | incorrect identification of recent asian strains of coxsackievirus a16 as human enterovirus 71: improved primers for the specific detection of human enterovirus 71 by rt pcr |
| topic | RJ101 Child Health. Child health services |
| url | http://ir.unimas.my/id/eprint/13620/ http://ir.unimas.my/id/eprint/13620/ http://ir.unimas.my/id/eprint/13620/ http://ir.unimas.my/id/eprint/13620/1/Incorrect%20identification%20of%20recent%20Asian%20strains%20of%20Coxsackievirus%20%28abstract%29.pdf |