Carbon nanodots as molecular scaffolds for development of antimicrobial agents
We report the potential of carbon nanodots (CNDs) as a molecular scaffold for enhancing the antimicrobial activities of small dendritic poly(amidoamines) (PAMAM). Carbon nanodots prepared from sago starch are readily functionalized with PAMAM by using N-ethyl-N0-(3-dimethylaminopropyl)carbodiimide...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier Ltd.
2016
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| Online Access: | http://ir.unimas.my/id/eprint/12986/ http://ir.unimas.my/id/eprint/12986/1/Carbon%20nanodots%20as%20molecular%20scaffolds%20for%20development%20%28abstract%29.pdf |
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| author | Maria, Ngu-Schwemlein Suk, Fun Chin Ryan, Hileman Chris, Drozdowski Clint, Upchurch April, Hargrove |
| author_facet | Maria, Ngu-Schwemlein Suk, Fun Chin Ryan, Hileman Chris, Drozdowski Clint, Upchurch April, Hargrove |
| author_sort | Maria, Ngu-Schwemlein |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | We report the potential of carbon nanodots (CNDs) as a molecular scaffold for enhancing the antimicrobial
activities of small dendritic poly(amidoamines) (PAMAM). Carbon nanodots prepared from sago
starch are readily functionalized with PAMAM by using N-ethyl-N0-(3-dimethylaminopropyl)carbodiimide
hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Electron microscopy images of these
polyaminated CNDs show that they are approximately 30–60 nm in diameter. Infrared and fluorescence
spectroscopy analyses of the water-soluble material established the presence of the polyamidoaminated
moiety and the intrinsic fluorescence of the nanodots. The polyaminated nanodots (CND-PAM1 and
CND-PAM2) exhibit in vitro antimicrobial properties, not only to non-multidrug resistant bacteria but
also to the corresponding Gram-negative multidrug bacteria. Their minimum inhibitory concentration
(MIC) ranges from 8 to 64 lg/mL, which is much lower than that of PAMAM G1 or the non-active
PAMAM G0 and CNDs. Additionally, they show synergistic effect in combination with tetracycline or
colistin. These preliminary results imply that CNDs can serve as a promising scaffold for facilitating
the rational design of antimicrobial materials for combating the ever-increasing threat of antibiotic resistance.
Moreover, their fluorescence could be pertinent to unraveling their mode of action for imaging or
diagnostic applications. |
| first_indexed | 2025-11-15T06:37:42Z |
| format | Article |
| id | unimas-12986 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:37:42Z |
| publishDate | 2016 |
| publisher | Elsevier Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-129862017-02-22T00:58:56Z http://ir.unimas.my/id/eprint/12986/ Carbon nanodots as molecular scaffolds for development of antimicrobial agents Maria, Ngu-Schwemlein Suk, Fun Chin Ryan, Hileman Chris, Drozdowski Clint, Upchurch April, Hargrove TP Chemical technology We report the potential of carbon nanodots (CNDs) as a molecular scaffold for enhancing the antimicrobial activities of small dendritic poly(amidoamines) (PAMAM). Carbon nanodots prepared from sago starch are readily functionalized with PAMAM by using N-ethyl-N0-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Electron microscopy images of these polyaminated CNDs show that they are approximately 30–60 nm in diameter. Infrared and fluorescence spectroscopy analyses of the water-soluble material established the presence of the polyamidoaminated moiety and the intrinsic fluorescence of the nanodots. The polyaminated nanodots (CND-PAM1 and CND-PAM2) exhibit in vitro antimicrobial properties, not only to non-multidrug resistant bacteria but also to the corresponding Gram-negative multidrug bacteria. Their minimum inhibitory concentration (MIC) ranges from 8 to 64 lg/mL, which is much lower than that of PAMAM G1 or the non-active PAMAM G0 and CNDs. Additionally, they show synergistic effect in combination with tetracycline or colistin. These preliminary results imply that CNDs can serve as a promising scaffold for facilitating the rational design of antimicrobial materials for combating the ever-increasing threat of antibiotic resistance. Moreover, their fluorescence could be pertinent to unraveling their mode of action for imaging or diagnostic applications. Elsevier Ltd. 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/12986/1/Carbon%20nanodots%20as%20molecular%20scaffolds%20for%20development%20%28abstract%29.pdf Maria, Ngu-Schwemlein and Suk, Fun Chin and Ryan, Hileman and Chris, Drozdowski and Clint, Upchurch and April, Hargrove (2016) Carbon nanodots as molecular scaffolds for development of antimicrobial agents. Bioorganic & Medicinal Chemistry Letters, 26 (7). pp. 1745-1749. ISSN 0960-894X https://www.scopus.com/record/display.uri?eid=2-s2.0-84979054298&origin=resultslist&sort=plf-f&src=s&st1=Carbon+nanodots+as+molecular+scaffolds+for+development+of+antimicrobial+agents&st2=&sid=B1B05F08DE62EFCA7D3CF5AD04BE357C.wsnAw8kcdt7IPYLO0V48gA%3a80&s http://dx.doi.org/10.1016/j.bmcl.2016.02.047 |
| spellingShingle | TP Chemical technology Maria, Ngu-Schwemlein Suk, Fun Chin Ryan, Hileman Chris, Drozdowski Clint, Upchurch April, Hargrove Carbon nanodots as molecular scaffolds for development of antimicrobial agents |
| title | Carbon nanodots as molecular scaffolds for development
of antimicrobial agents |
| title_full | Carbon nanodots as molecular scaffolds for development
of antimicrobial agents |
| title_fullStr | Carbon nanodots as molecular scaffolds for development
of antimicrobial agents |
| title_full_unstemmed | Carbon nanodots as molecular scaffolds for development
of antimicrobial agents |
| title_short | Carbon nanodots as molecular scaffolds for development
of antimicrobial agents |
| title_sort | carbon nanodots as molecular scaffolds for development
of antimicrobial agents |
| topic | TP Chemical technology |
| url | http://ir.unimas.my/id/eprint/12986/ http://ir.unimas.my/id/eprint/12986/ http://ir.unimas.my/id/eprint/12986/ http://ir.unimas.my/id/eprint/12986/1/Carbon%20nanodots%20as%20molecular%20scaffolds%20for%20development%20%28abstract%29.pdf |