The Genetic Variation A > G at 3' UTR of Nuclear Factor Kappa B 1 A (NFkB1A) Influences Susceptibility of Sporadic Colorectal Cancer in Malaysian Population
Colorectal Cancer (CRC), represents a significant cause of morbidity and mortality worldwide. Its incidence is increasing in developed and developing countries including Malaysia. Multiple disease pathways including Nuclear Factor Kappa B (NFkB) signaling pathways, have been implicated in Colorect...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Japan International Cultural Exchange Foundation & Japan Health Sciences University
2012
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| Online Access: | http://ir.unimas.my/id/eprint/1294/ http://ir.unimas.my/id/eprint/1294/1/the%20genetic%20variation.pdf |
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| author | Mohd Suzairi, Mohd Shafi'i Siti Nurfatimah, Mohd Shahpudin Mohd Aminuddin, Mustapha Ahmad Aizat, Abdul Aziz Biswal, Biswal Mohan Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hassan Ankathil, Ravindran |
| author_facet | Mohd Suzairi, Mohd Shafi'i Siti Nurfatimah, Mohd Shahpudin Mohd Aminuddin, Mustapha Ahmad Aizat, Abdul Aziz Biswal, Biswal Mohan Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hassan Ankathil, Ravindran |
| author_sort | Mohd Suzairi, Mohd Shafi'i |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Colorectal Cancer (CRC), represents a significant cause of morbidity and mortality worldwide. Its incidence is increasing in
developed and developing countries including Malaysia. Multiple disease pathways including Nuclear Factor Kappa B (NFkB)
signaling pathways, have been implicated in Colorectal carcinogenesis. Given the important role of NFkB pathway in Colorectal
carcinogenesis, genes and genetic variations influencing NFkB signaling pathway could be candidate CRC predisposition factors.
We hypothesized that an A to G variation in the 3' UTR of NFkB1A may be associated with CRC susceptibility risk in
Malaysian population.
Design: A Case - Control study was designed to investigate the genotype frequencies of A to G polymorphism at 3' UTR of
NFkB1A in Malaysian CRC patients and normal Controls and to determine the genetic risk association of the variant genotype
on CRC susceptibility.
Objectives: To investigate the genotype frequencies of A to G polymorphism at 3' UTR of NFkBIA in Malaysian sporadic
CRC patients and healthy controls and to determine the association risk of the variant genotype on CRC susceptibility.
Materials and Methods: This study involved 510 subjects with 211 histopathologically confirmed CRC patients as Cases and
299 healthy individuals as Controls. Blood samples from study subjects were collected, DNA extracted and genotyped employing
PCR-RFLP technique. Risk associations of specific genotypes with CRC susceptibility were determined by computing Odds
Ratios (ORs) and 95% Confidence Intervals (CI).
Results: The frequency of homozygous major (AA) genotype was significantly higher (p = 0.001) among Controls (44.8%)
compared to CRC patients (31.8%) but heterozygous genotype (AG) showed no significant difference between cases and controls.
However, the frequency of homozygous variant (GG) genotype was significantly higher in CRC cases (40.7%) compared to
controls (24.4%). On investigating the risk, the variant genotype GG showed significant risk association with CRC susceptibility
(OR = 2.356, CI: 1.536 - 3.615, p = 0.001).
Conclusion: From the results, it is reasonable to suggest that the A to G variation in the 3' UTR of NFkB1A could be a predisposition
risk factor in colorectal carcinogenesis, mediating through NFkB signaling pathway. |
| first_indexed | 2025-11-15T05:56:40Z |
| format | Article |
| id | unimas-1294 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T05:56:40Z |
| publishDate | 2012 |
| publisher | Japan International Cultural Exchange Foundation & Japan Health Sciences University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-12942015-11-02T07:20:02Z http://ir.unimas.my/id/eprint/1294/ The Genetic Variation A > G at 3' UTR of Nuclear Factor Kappa B 1 A (NFkB1A) Influences Susceptibility of Sporadic Colorectal Cancer in Malaysian Population Mohd Suzairi, Mohd Shafi'i Siti Nurfatimah, Mohd Shahpudin Mohd Aminuddin, Mustapha Ahmad Aizat, Abdul Aziz Biswal, Biswal Mohan Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hassan Ankathil, Ravindran Q Science (General) R Medicine (General) Colorectal Cancer (CRC), represents a significant cause of morbidity and mortality worldwide. Its incidence is increasing in developed and developing countries including Malaysia. Multiple disease pathways including Nuclear Factor Kappa B (NFkB) signaling pathways, have been implicated in Colorectal carcinogenesis. Given the important role of NFkB pathway in Colorectal carcinogenesis, genes and genetic variations influencing NFkB signaling pathway could be candidate CRC predisposition factors. We hypothesized that an A to G variation in the 3' UTR of NFkB1A may be associated with CRC susceptibility risk in Malaysian population. Design: A Case - Control study was designed to investigate the genotype frequencies of A to G polymorphism at 3' UTR of NFkB1A in Malaysian CRC patients and normal Controls and to determine the genetic risk association of the variant genotype on CRC susceptibility. Objectives: To investigate the genotype frequencies of A to G polymorphism at 3' UTR of NFkBIA in Malaysian sporadic CRC patients and healthy controls and to determine the association risk of the variant genotype on CRC susceptibility. Materials and Methods: This study involved 510 subjects with 211 histopathologically confirmed CRC patients as Cases and 299 healthy individuals as Controls. Blood samples from study subjects were collected, DNA extracted and genotyped employing PCR-RFLP technique. Risk associations of specific genotypes with CRC susceptibility were determined by computing Odds Ratios (ORs) and 95% Confidence Intervals (CI). Results: The frequency of homozygous major (AA) genotype was significantly higher (p = 0.001) among Controls (44.8%) compared to CRC patients (31.8%) but heterozygous genotype (AG) showed no significant difference between cases and controls. However, the frequency of homozygous variant (GG) genotype was significantly higher in CRC cases (40.7%) compared to controls (24.4%). On investigating the risk, the variant genotype GG showed significant risk association with CRC susceptibility (OR = 2.356, CI: 1.536 - 3.615, p = 0.001). Conclusion: From the results, it is reasonable to suggest that the A to G variation in the 3' UTR of NFkB1A could be a predisposition risk factor in colorectal carcinogenesis, mediating through NFkB signaling pathway. Japan International Cultural Exchange Foundation & Japan Health Sciences University 2012 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/1294/1/the%20genetic%20variation.pdf Mohd Suzairi, Mohd Shafi'i and Siti Nurfatimah, Mohd Shahpudin and Mohd Aminuddin, Mustapha and Ahmad Aizat, Abdul Aziz and Biswal, Biswal Mohan and Zaidi, Zakaria and Ahmad Shanwani, Mohd Sidek and Mohammad Radzi, Abu Hassan and Ankathil, Ravindran (2012) The Genetic Variation A > G at 3' UTR of Nuclear Factor Kappa B 1 A (NFkB1A) Influences Susceptibility of Sporadic Colorectal Cancer in Malaysian Population. International Medical Journal, 19 (2). pp. 98-101. ISSN 1341-2051 |
| spellingShingle | Q Science (General) R Medicine (General) Mohd Suzairi, Mohd Shafi'i Siti Nurfatimah, Mohd Shahpudin Mohd Aminuddin, Mustapha Ahmad Aizat, Abdul Aziz Biswal, Biswal Mohan Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hassan Ankathil, Ravindran The Genetic Variation A > G at 3' UTR of Nuclear Factor Kappa B 1 A (NFkB1A) Influences Susceptibility of Sporadic Colorectal Cancer in Malaysian Population |
| title | The Genetic Variation A > G at 3' UTR of Nuclear Factor Kappa B 1 A (NFkB1A) Influences Susceptibility of Sporadic Colorectal Cancer in Malaysian Population |
| title_full | The Genetic Variation A > G at 3' UTR of Nuclear Factor Kappa B 1 A (NFkB1A) Influences Susceptibility of Sporadic Colorectal Cancer in Malaysian Population |
| title_fullStr | The Genetic Variation A > G at 3' UTR of Nuclear Factor Kappa B 1 A (NFkB1A) Influences Susceptibility of Sporadic Colorectal Cancer in Malaysian Population |
| title_full_unstemmed | The Genetic Variation A > G at 3' UTR of Nuclear Factor Kappa B 1 A (NFkB1A) Influences Susceptibility of Sporadic Colorectal Cancer in Malaysian Population |
| title_short | The Genetic Variation A > G at 3' UTR of Nuclear Factor Kappa B 1 A (NFkB1A) Influences Susceptibility of Sporadic Colorectal Cancer in Malaysian Population |
| title_sort | genetic variation a > g at 3' utr of nuclear factor kappa b 1 a (nfkb1a) influences susceptibility of sporadic colorectal cancer in malaysian population |
| topic | Q Science (General) R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/1294/ http://ir.unimas.my/id/eprint/1294/1/the%20genetic%20variation.pdf |