A Preliminary Study on the Induction of Somatic Embryogenesis of Eusideroxylonzwageri Tesym and Binned (Borneo Ironwood) from Leaf Explant

Eusideroxylon zwageri is a tree of tropical rainforest zone and belongs to a Lauraceae family. It is one of the hardest timber species in Southeast Asia. The objective of this study was to determine the optimal culture medium for the induction of somatic embryogenesis f explants of E. zwageri....

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Main Authors: Gibson, E., Rebicca, E.
Format: Article
Language:English
Published: IJAIR 2016
Subjects:
Online Access:http://ir.unimas.my/id/eprint/12877/
http://ir.unimas.my/id/eprint/12877/1/A%20Preliminary%20Study%20on%20the%20Induction%20of%20Somatic%20%28abstract%29.pdf
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author Gibson, E.
Rebicca, E.
author_facet Gibson, E.
Rebicca, E.
author_sort Gibson, E.
building UNIMAS Institutional Repository
collection Online Access
description Eusideroxylon zwageri is a tree of tropical rainforest zone and belongs to a Lauraceae family. It is one of the hardest timber species in Southeast Asia. The objective of this study was to determine the optimal culture medium for the induction of somatic embryogenesis f explants of E. zwageri. It was found that the globular somatic embryos were induced in half strength of MS medium with 1.0, 1.5, 2.0 mg/L of BAP in combination with either 0.5 mg/L of 2,4-D or NAA. The maturation of somatic embryos obtained in half strength of MS medium with BAP, NAA and GA3. The highest mean number of the induction of somatic embryos up to the cotyledonary phase was observed in culture medium containing 1.0 mg/L of BAP, 0.5 mg/L of NAA in combination with 1.0 mg/L of GA reported preliminary study was useful for further plantlet regeneration of this species through induction of somatic embryogenesis.
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spelling unimas-128772016-08-10T20:05:40Z http://ir.unimas.my/id/eprint/12877/ A Preliminary Study on the Induction of Somatic Embryogenesis of Eusideroxylonzwageri Tesym and Binned (Borneo Ironwood) from Leaf Explant Gibson, E. Rebicca, E. GE Environmental Sciences SB Plant culture Eusideroxylon zwageri is a tree of tropical rainforest zone and belongs to a Lauraceae family. It is one of the hardest timber species in Southeast Asia. The objective of this study was to determine the optimal culture medium for the induction of somatic embryogenesis f explants of E. zwageri. It was found that the globular somatic embryos were induced in half strength of MS medium with 1.0, 1.5, 2.0 mg/L of BAP in combination with either 0.5 mg/L of 2,4-D or NAA. The maturation of somatic embryos obtained in half strength of MS medium with BAP, NAA and GA3. The highest mean number of the induction of somatic embryos up to the cotyledonary phase was observed in culture medium containing 1.0 mg/L of BAP, 0.5 mg/L of NAA in combination with 1.0 mg/L of GA reported preliminary study was useful for further plantlet regeneration of this species through induction of somatic embryogenesis. IJAIR 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/12877/1/A%20Preliminary%20Study%20on%20the%20Induction%20of%20Somatic%20%28abstract%29.pdf Gibson, E. and Rebicca, E. (2016) A Preliminary Study on the Induction of Somatic Embryogenesis of Eusideroxylonzwageri Tesym and Binned (Borneo Ironwood) from Leaf Explant. International Journal of Agriculture Innovations and Research, 4 (6). ISSN 2319-1473 http://ijair.org/index.php/issues?view=publication&task=show&id=756
spellingShingle GE Environmental Sciences
SB Plant culture
Gibson, E.
Rebicca, E.
A Preliminary Study on the Induction of Somatic Embryogenesis of Eusideroxylonzwageri Tesym and Binned (Borneo Ironwood) from Leaf Explant
title A Preliminary Study on the Induction of Somatic Embryogenesis of Eusideroxylonzwageri Tesym and Binned (Borneo Ironwood) from Leaf Explant
title_full A Preliminary Study on the Induction of Somatic Embryogenesis of Eusideroxylonzwageri Tesym and Binned (Borneo Ironwood) from Leaf Explant
title_fullStr A Preliminary Study on the Induction of Somatic Embryogenesis of Eusideroxylonzwageri Tesym and Binned (Borneo Ironwood) from Leaf Explant
title_full_unstemmed A Preliminary Study on the Induction of Somatic Embryogenesis of Eusideroxylonzwageri Tesym and Binned (Borneo Ironwood) from Leaf Explant
title_short A Preliminary Study on the Induction of Somatic Embryogenesis of Eusideroxylonzwageri Tesym and Binned (Borneo Ironwood) from Leaf Explant
title_sort preliminary study on the induction of somatic embryogenesis of eusideroxylonzwageri tesym and binned (borneo ironwood) from leaf explant
topic GE Environmental Sciences
SB Plant culture
url http://ir.unimas.my/id/eprint/12877/
http://ir.unimas.my/id/eprint/12877/
http://ir.unimas.my/id/eprint/12877/1/A%20Preliminary%20Study%20on%20the%20Induction%20of%20Somatic%20%28abstract%29.pdf