Development of single chain variable fragment antibodies against dengue virus
The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned in...
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| Format: | Thesis |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2006
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| Online Access: | http://ir.unimas.my/id/eprint/12719/ http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf |
| _version_ | 1848837259832328192 |
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| author | Leong, Yee Wei. |
| author_facet | Leong, Yee Wei. |
| author_sort | Leong, Yee Wei. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned into a phagemid vector (pCANTAB 5 E) and expressed as a fusion protein with M13 phage in E.coli TG1. Affinity-selection was performed to select and enich scFv phages with strong binding to their respective antigens. |
| first_indexed | 2025-11-15T06:36:49Z |
| format | Thesis |
| id | unimas-12719 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:36:49Z |
| publishDate | 2006 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-127192023-03-07T08:40:15Z http://ir.unimas.my/id/eprint/12719/ Development of single chain variable fragment antibodies against dengue virus Leong, Yee Wei. RA0421 Public health. Hygiene. Preventive Medicine The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned into a phagemid vector (pCANTAB 5 E) and expressed as a fusion protein with M13 phage in E.coli TG1. Affinity-selection was performed to select and enich scFv phages with strong binding to their respective antigens. Universiti Malaysia Sarawak, (UNIMAS) 2006 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf Leong, Yee Wei. (2006) Development of single chain variable fragment antibodies against dengue virus. Masters thesis, Institute of Health andCommunity Medicine. |
| spellingShingle | RA0421 Public health. Hygiene. Preventive Medicine Leong, Yee Wei. Development of single chain variable fragment antibodies against dengue virus |
| title | Development of single chain variable fragment antibodies against dengue virus |
| title_full | Development of single chain variable fragment antibodies against dengue virus |
| title_fullStr | Development of single chain variable fragment antibodies against dengue virus |
| title_full_unstemmed | Development of single chain variable fragment antibodies against dengue virus |
| title_short | Development of single chain variable fragment antibodies against dengue virus |
| title_sort | development of single chain variable fragment antibodies against dengue virus |
| topic | RA0421 Public health. Hygiene. Preventive Medicine |
| url | http://ir.unimas.my/id/eprint/12719/ http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf |