High diversity of Pseudo-nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae)

Forty-eight isolates of Pseudo-nitzschia species were established from the Miri coast of Sarawak (Malaysian Borneo) and underwent TEM observation and molecular characterization. Ten species were found: P. abrensis, P. batesiana, P. fukuyoi, P. kodamae, P. lundholmiae, P. multistriata, P. pungens,...

Full description

Bibliographic Details
Main Authors: Sing, Tung Teng, Suh, Nih Tan, Hong, Chang Lim, Viet, Ha Dao
Format: Article
Language:English
Published: Phycological Society of America 2016
Subjects:
Online Access:http://ir.unimas.my/id/eprint/12657/
http://ir.unimas.my/id/eprint/12657/1/HIGH%20DIVERSITY%20-%20Copy.pdf
_version_ 1848837244993929216
author Sing, Tung Teng
Suh, Nih Tan
Hong, Chang Lim
Viet, Ha Dao
author_facet Sing, Tung Teng
Suh, Nih Tan
Hong, Chang Lim
Viet, Ha Dao
author_sort Sing, Tung Teng
building UNIMAS Institutional Repository
collection Online Access
description Forty-eight isolates of Pseudo-nitzschia species were established from the Miri coast of Sarawak (Malaysian Borneo) and underwent TEM observation and molecular characterization. Ten species were found: P. abrensis, P. batesiana, P. fukuyoi, P. kodamae, P. lundholmiae, P. multistriata, P. pungens, P. subfraudulenta, as well as two additional new morphotypes, herein designated as P. bipertita sp. nov. and P. limii sp. nov. This is the first report of P. abrensis, P. batesiana, P. kodamae, P. fukuyoi and P. lundholmiae in coastal waters of Malaysian Borneo. Pseudo-nitzschia bipertita differs from its congeners by the number of sectors that divide the poroids, densities of band striae and its cingular band structure. Pseudo-nitzschia limii, a pseudo-cryptic species in the P. seudodelicatissima complex sensu lato, is distinct by having wider proximal and distal mantles, a higher number of striae and greater poroid height in the striae of the valvocopula. The species were further supported by the phylogenetic reconstructions of the nuclear-encoded large subunit ribosomal gene and the second internal transcribed spacer. Phylogenetically, P. bipertita clustered with its sister taxa (P. subpacifica+P. heimii); P. limii appears as a sister taxon to P. kodamae and P. hasleana in the ITS2 tree. Pairwise comparison of ITS2 transcripts with its closest relatives revealed the presence of both hemi- and compensatory base changes. Toxicity analysis showed detectable levels of domoic acid in P. abrensis, P. batesiana, P. lundholmiae and P. ubfraudulenta, but both new species tested below the detection limit.
first_indexed 2025-11-15T06:36:35Z
format Article
id unimas-12657
institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:36:35Z
publishDate 2016
publisher Phycological Society of America
recordtype eprints
repository_type Digital Repository
spelling unimas-126572022-06-07T06:40:02Z http://ir.unimas.my/id/eprint/12657/ High diversity of Pseudo-nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae) Sing, Tung Teng Suh, Nih Tan Hong, Chang Lim Viet, Ha Dao Q Science (General) Forty-eight isolates of Pseudo-nitzschia species were established from the Miri coast of Sarawak (Malaysian Borneo) and underwent TEM observation and molecular characterization. Ten species were found: P. abrensis, P. batesiana, P. fukuyoi, P. kodamae, P. lundholmiae, P. multistriata, P. pungens, P. subfraudulenta, as well as two additional new morphotypes, herein designated as P. bipertita sp. nov. and P. limii sp. nov. This is the first report of P. abrensis, P. batesiana, P. kodamae, P. fukuyoi and P. lundholmiae in coastal waters of Malaysian Borneo. Pseudo-nitzschia bipertita differs from its congeners by the number of sectors that divide the poroids, densities of band striae and its cingular band structure. Pseudo-nitzschia limii, a pseudo-cryptic species in the P. seudodelicatissima complex sensu lato, is distinct by having wider proximal and distal mantles, a higher number of striae and greater poroid height in the striae of the valvocopula. The species were further supported by the phylogenetic reconstructions of the nuclear-encoded large subunit ribosomal gene and the second internal transcribed spacer. Phylogenetically, P. bipertita clustered with its sister taxa (P. subpacifica+P. heimii); P. limii appears as a sister taxon to P. kodamae and P. hasleana in the ITS2 tree. Pairwise comparison of ITS2 transcripts with its closest relatives revealed the presence of both hemi- and compensatory base changes. Toxicity analysis showed detectable levels of domoic acid in P. abrensis, P. batesiana, P. lundholmiae and P. ubfraudulenta, but both new species tested below the detection limit. Phycological Society of America 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/12657/1/HIGH%20DIVERSITY%20-%20Copy.pdf Sing, Tung Teng and Suh, Nih Tan and Hong, Chang Lim and Viet, Ha Dao (2016) High diversity of Pseudo-nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae). Journal of Phycology, 52 (3). ISSN 1529-8817 http://onlinelibrary.wiley.com/doi/10.1111/jpy.12448/abstract doi: 10.1111/jpy.12448-15-095
spellingShingle Q Science (General)
Sing, Tung Teng
Suh, Nih Tan
Hong, Chang Lim
Viet, Ha Dao
High diversity of Pseudo-nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae)
title High diversity of Pseudo-nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae)
title_full High diversity of Pseudo-nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae)
title_fullStr High diversity of Pseudo-nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae)
title_full_unstemmed High diversity of Pseudo-nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae)
title_short High diversity of Pseudo-nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae)
title_sort high diversity of pseudo-nitzschia along the northern coast of sarawak (malaysian borneo), with descriptions of p. bipertita sp. nov. and p. limii sp. nov. (bacillariophyceae)
topic Q Science (General)
url http://ir.unimas.my/id/eprint/12657/
http://ir.unimas.my/id/eprint/12657/
http://ir.unimas.my/id/eprint/12657/
http://ir.unimas.my/id/eprint/12657/1/HIGH%20DIVERSITY%20-%20Copy.pdf